Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Short and long-term immune effects of Poly (I:C) in kidney of Olive flounder (Paralichthys olivaceus)

넙치(Paralichthys olivaceus) 신장에서 Poly (I:C)의 단기 및 장기적인 면역 효과

  • Minjae Seong (Department of Aquatic Life Medical Sciences, Sunmoon University) ;
  • Youngjin Park (Department of Aquatic Life Medical Sciences, Sunmoon University)
  • 성민재 (선문대학교 수산생명의학과) ;
  • 박영진 (선문대학교 수산생명의학과)
  • Received : 2024.05.30
  • Accepted : 2024.06.13
  • Published : 2024.06.30
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Abstract

Viral diseases cause enormous economic losses to the olive flounder (Paralichthys olivaceus) aquaculture industry in Korea. This study aimed to identify immune-related genes expressed in the kidney of olive flounder injected with Polyinosinic-polycytidylic acid (Poly (I:C)). Thirty fish were divided into two groups by intraperitoneal injection of 100µl of diethylpyrocarbonate-treated water or poly I:C per fish. Kidney tissues at day 3 and 30 after the injection were used for RNA-seq analysis to identify differentially expressed genes (DEGs). Poly I:C group upregulated il8, cfh, tnfaip2b, c3b.2, ly6d and cd38 genes at 3 days post-injection. Additionally, cd22, ccl34a.3, c9, cxcl19, ccl27a, ccl7, and cfh genes were upregulated at 30 days post-injection. Differential expression gene analysis showed that poly I:C has both short and long-term immune effects in olive flounder. This study provides a theoretical basis for understanding the molecular mechanism of the short and long-term immune effects of poly I:C.

Keywords

Acknowledgement

이 논문은 2023년도 정부(교육부)의 재원으로 한국연구재단 기초연구사업의 지원을 받아 수행된 연구임(RS-2023-00251522).

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