Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Development of antibodies after combination Streptococcus iniae and Streptococcus parauberis of commercial vaccine injection in olive flounder (Paralichthys olivaceus) in farm

양식 넙치에서 연쇄구균 혼합백신 접종 후 항체가 변화 조사

  • Hyun-Ja Han (Pathology Division, National Institute of Fisheries Science) ;
  • Deok Chan Lee (Southeast Sea Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Soo-Jin Kim (Pathology Division, National Institute of Fisheries Science) ;
  • Tae-Ho Kim (Pathology Division, National Institute of Fisheries Science) ;
  • Sun-Myoung Jeong (Pathology Division, National Institute of Fisheries Science) ;
  • Jae-Hwi Kim (Changjo Biotech Inc) ;
  • Yoon-Jea Choi (Wando aquatic life medicine) ;
  • Kyeong-Yong Cho (Shinil aquatic life medicine) ;
  • Mi-Young Cho (Pathology Division, National Institute of Fisheries Science)
  • 한현자 (국립수산과학원 병리연구과) ;
  • 이덕찬 (국립수산과학원 남동해수산연구소) ;
  • 김수진 (국립수산과학원 병리연구과) ;
  • 김태호 (국립수산과학원 병리연구과) ;
  • 정선명 (국립수산과학원 병리연구과) ;
  • 김재휘 ((주)창조바이오텍) ;
  • 최윤재 (완도수산질병관리원) ;
  • 조경용 (신일수산질병관리원) ;
  • 조미영 (국립수산과학원 병리연구과)
  • Received : 2024.05.14
  • Accepted : 2024.05.30
  • Published : 2024.06.30
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Abstract

Streptococcosis, caused by Streptococcus iniae and Streptococcus parauberis is an important bacterial disease that affects in olive flounder in Korea. In Korea, multivalent bacterial vaccines are used to prevent streptococcal diseases in aquaculture. In this study, commercial vaccines containing formalin-inactivated bacterial cells of S. iniae and S. parauberis were administered at six fish farms and one unvaccinated fish farm were designated for investigation (Wando; 4 sites and Jeju; 3 sites). Blood was collected from vaccinated and unvaccinated olive flounders, and titers of antibodies against S. iniae and S. parauberis in serum were analyzed using ELISA. After a one shot vaccination in the farms at Jeju (farm A) and Wando (farm D), the proportion of individuals with specific antibodies against S. parauberis OD values of 0.4 or higher was 60% and 53.5%, respectively. But after booster vaccination, the proportion of individuals with serum OD values of 0.4 or higher was higher substantially increased to 96.6% (farm A) and 100% (farm D). The levels of S. parauberis specific antibodies of olive flounder were increased after vaccination in three fish farms (farm D, E, and F), but not S. iniae specific antibodies.

Keywords

Acknowledgement

본 연구는 국립수산과학원 수산시험연구사업(R2024055)의 지원에 의해서 운영되었습니다.

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