Journal of Convergence Korean Medicine (대한융합한의학회지)

Academy of Convergence Korean Medicine (대한융합한의학회)
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Effect of Daehwangmokdanpitang on psoriasis-like skin inflammation

건선 모사 피부 염증모델에서 대황목단피탕(大黃牧丹皮湯)의 효능 연구

  • Gyeong-Ran Noh (Department of Pharmacology, College of Korean Medicine, Wonkwang University) ;
  • Bitna Kweon (Department of Pharmacology, College of Korean Medicine, Wonkwang University) ;
  • Dong-Uk Kim (Department of Pharmacology, College of Korean Medicine, Wonkwang University) ;
  • Jin‑Young Oh (Department of Pharmacology, College of Korean Medicine, Wonkwang University) ;
  • Gabsik Yang (Department of Pharmacology, College of Korean Medicine, Woosuk University) ;
  • Il-Joo Jo (Central Stroke Center of Korean medicine, College of Korean Medicine, Wonkwang University)
  • 노경란 (원광대학교 한의과대학 약리학교실) ;
  • 권빛나 (원광대학교 한의과대학 약리학교실) ;
  • 김동욱 (원광대학교 한의과대학 약리학교실) ;
  • 오진영 (원광대학교 한의과대학 약리학교실) ;
  • 양갑식 (우석대학교 한의과대학 임상약리연구실) ;
  • 조일주 (원광대학교 한의과대학 뇌졸중한의중점연구센터)
  • Published : 2024.06.30
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Abstract

Objectives: Psoriasis is a common chronic inflammatory skin disease characterized by keratinocyte hyperproliferation and an excessive inflammatory response. Agents that can attenuate keratinocyte hyperproliferation and excessive inflammatory responses are considered potentially useful for the treatment of psoriasis. Daehwangmokdanpitang (DHMDPT) exhibits a broad range of bioactivities, including anti-proliferative and anti-inflammatory effects. This study aims to evaluate the anti-psoriatic potential of DHMDPT in vitro. Methods: HaCaT keratinocytes were stimulated with a mixture of IL-17A, IL-22, oncostatin M, IL-1α, and TNF-α (M5) to establish an in vitro psoriatic keratinocyte model. Cell viability was measured using the MTT assay. Quantitative real-time PCR (qRT-PCR) was performed to measure the mRNA levels of the hyperproliferative marker gene keratin 6 (KRT6) and inflammatory factors such as IL-6, TNF-α, and IL-23A. Additionally, chemokines including CCL5, CCL2, CCL20, and CXCL1 were measured by qRT-PCR. Results: DHMDPT attenuated M5-induced hyperproliferation, as indicated by a reduction in KRT6 expression in HaCaT keratinocytes. M5 stimulation significantly upregulated the mRNA levels of IL-6, TNF-α, and IL-23A. However, DHMDPT treatment attenuated the upregulation of IL-6 but not TNF-α or IL-23A. Additionally, DHMDPT inhibited the expression of CCL5, CCL2, and CXCL1, but not CCL20. Conclusion: DHMDPT effectively attenuated the M5-induced proliferation and inflammatory response in HaCaT keratinocytes. Therefore, DHMDPT could be an attractive candidate for future development as an anti-psoriatic agent.

Keywords

Acknowledgement

본 연구는 과학기술정보통신부의 재원으로 한국연구재단의 이공학학술연구기반구축(R&D) 지역대학 우수과학자 지원사업(RS-2023-00248541)과 보건복지부의 재원으로 한국보건산업진흥원의 보건의료 기술연구개발사업 지원(HF22C0072)에 의하여 이루어짐.

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