Advances in materials Research

  • 제13권3호
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  • Pages.233-241
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  • 2024
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  • 2234-0912(pISSN)
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  • 2234-179X(eISSN)
테크노프레스 (Techno-Press)
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Quantitative analysis method for zingiber officinale water extract using high-performance liquid chromatography

  • Mohd S. Md Sarip (Faculty of Chemical Engineering and Technology, Kompleks Pusat Pengajian Jejawi 3, Universiti Malaysia Perlis (UniMAP)) ;
  • Nik M.A. Nik Daud (Faculty of Chemical Engineering and Technology, Kompleks Pusat Pengajian Jejawi 3, Universiti Malaysia Perlis (UniMAP)) ;
  • Mohd A. Mohd Zainudin (Faculty of Chemical Engineering and Technology, Kompleks Pusat Pengajian Jejawi 3, Universiti Malaysia Perlis (UniMAP)) ;
  • Lokman H. Ibrahim (Faculty of Chemical Engineering and Technology, Kompleks Pusat Pengajian Jejawi 3, Universiti Malaysia Perlis (UniMAP)) ;
  • Syahrul A. Saidi (Faculty of Electrical Engineering & Technology, UniCITI Alam Campus, Universiti Malaysia Perlis (UniMAP)) ;
  • Zuhaili Idham (Department of Deputy Vice-Chancellor (Research and Innovation), Universiti Teknologi Malaysia) ;
  • Adilah Anuar (Faculty of Chemical Engineering and Technology, Kompleks Pusat Pengajian Jejawi 3, Universiti Malaysia Perlis (UniMAP))
  • 투고 : 2023.07.15
  • 심사 : 2024.01.03
  • 발행 : 2024.06.25
⟨ 이전 논문 다음 논문 ⟩

초록

Quantitative analysis of the Zingiber Officinale sample using subcritical water extraction (SWE) was developed employing High-Performance Liquid Chromatography (HPLC) to bolster the advancement of this innovative green extraction process. This research focuses on three principal ginger bioactive compounds: 6-gingerol, 6-shagoal, and 10-gingerol. Various stages were undertaken to establish the quantitative analysis method, encompassing the optimisation of HPLC operating conditions and the formulation of standard calibration curves, yielding individual compound equations. A robust correlation within the calibration curve was achieved, exhibiting an r2 value of 0.9814 and an RSD of 5.00%. A simultaneous, swift, and dependable method was established with an injection time of 20 minutes and an 8-minute delay between injections, in contrast to the previous HPLC analysis requiring a 45-minute injection time for detecting and quantifying all components. Notably, no post-treatment was applied after the SWE process. This advancement allows for potential future online measurement of Zingiber Officinale bioactive compounds extracted using subcritical water extraction through this technology.

키워드

과제정보

The research infrastructure provide by Universiti Teknologi Malaysia is greatly appreciated. Other individuals who have contributed to this research are also duly acknowledged.

참고문헌

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