대한소아치과학회지 (Journal of the korean academy of Pediatric Dentistry)

  • 제51권2호
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  • Pages.149-164
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  • 2024
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  • 1226-8496(pISSN)
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  • 2288-3819(eISSN)
대한소아치과학회 (Korean Academy of Pediatric Dentistry)
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Comparative Analysis of the Physical and Biochemical Properties of Light-cure Resin-modified Pulp Capping Materials

  • Tae Gyeom Kim (Department of Pediatric Dentistry, College of Dentistry, Dankook University) ;
  • Jongsoo Kim (Department of Pediatric Dentistry, College of Dentistry, Dankook University) ;
  • Joonhaeng Lee (Department of Pediatric Dentistry, College of Dentistry, Dankook University) ;
  • Jisun Shin (Department of Pediatric Dentistry, College of Dentistry, Dankook University) ;
  • Mi Ran Han (Department of Pediatric Dentistry, College of Dentistry, Dankook University) ;
  • Jongbin Kim (Department of Pediatric Dentistry, College of Dentistry, Dankook University) ;
  • Yujin Kim (Department of Biomaterials Science, College of Dentistry, Dankook University) ;
  • Jae Hee Park (Institute of Tissue Regeneration Engineering (ITREN), Dankook University)
  • 투고 : 2024.02.08
  • 심사 : 2024.04.05
  • 발행 : 2024.05.31
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초록

This study compared the solubility, water absorption, dimensional stability, release of various ions (hydroxyl, calcium, sulfur, strontium, and silicon), and cytotoxicity of light-cured resin-modified pulp-capping materials. Resin-modified calcium hydroxide (Ultra-blendTM plus, UBP), light-cured resin-modified calcium silicate (TheraCal LCTM, TLC), and dual-cure resin-modified calcium silicate (TheraCal PTTM, TPT) were used. Each material was polymerized; solubility, 24-hour water absorption, and 30- day dimensional stability experiments were conducted to test its physical properties. Solubility was assessed according to the ISO 6876 standard, and 24 hours of water absorption, 30 days of dimensional stability were assessed by referring to the previous protocol respectively. Eluates at 3 and 24 hours and on 7, 14, and 28 days were analyzed according to the ISO 10993-12 standard. And the pH, Ion-releasing ability, cell proliferation rate, and cell viability were assessed using the eluates to evaluate biochemical characteristics. pH was measured with a pH meter and Ion-releasing ability was assessed using inductively coupled plasma atomic emission spectrometry (ICP-AES). Cell proliferation rate and cell viability were assessed using human dental pulp cells (hDPCs). The former was assessed by an absorbance assay using the CCK-8 solution, and the latter was assessed by Live and Dead staining. TPT exhibited lower solubility and water absorption than TLC. UBP and TPT demonstrated higher stability than TLC. The release of sulfur, strontium, calcium, and hydroxyl ions was higher for TLC and TPT than for UBP. The 28-day release of hydroxyl and silicon ions was similar for TLC and TPT. TLC alone exhibited a lower cell proliferation rate compared to the control group at a dilution ratio of 1 : 2 in cell proliferation and dead cells from Live and Dead assay evaluation. Thus, when using light-cure resin-modified pulp-capping materials, calcium silicate-based materials can be considered alternatives to calcium hydroxide-based materials. Moreover, when comparing physical and biochemical properties, TPT could be prioritized over TLC as the first choice.

키워드

과제정보

This research was funded by the Department of Dentistry (Pediatric Dentistry), the Research-Focused Department Promotion Project as part of the University Innovation Support Program for Dankook University in 2021(2020R1G1A1009155), and the Basic Science Research Program funded by the Ministry of Education (NRF-2022R1I1A1A01069606).

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