Clinical and Experimental Reproductive Medicine

The Korean Society for Reproductive Medicine (대한생식의학회)
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The protective effect of zinc oxide and selenium oxide nanoparticles on the functional parameters of rat sperm during vitrification

  • Nafiseh Tavakolpoor Saleh (Depaetment of Biophysics and Biochemistry, Faculty of Advanced Science and Technology, Islamic Azad University Tehran Medical Sciences) ;
  • Zohreh Hosseinzadeh (Department of Nursing and Midwifery, Ilam University of Medical Sciences) ;
  • Narges Gholami Banadkuki (Andrology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences) ;
  • Maryam Salehi Novin (Department of Anatomy, Iran University of Medical Sciences) ;
  • Sanaz Saljooghi Zaman (Department of Embryology, Royan Institute for Reproductive Biomedicine, Royan Institute) ;
  • Tohid Moradi Gardeshi (Department of Veterinary Sciences, Garmsar Branch, Islamic Azad University)
  • Received : 2023.04.18
  • Accepted : 2023.08.28
  • Published : 2024.03.31
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Abstract

Objective: While sperm freezing (cryopreservation) is an effective method for preserving fertility, it can potentially harm the structure and function of sperm due to an increase in the production of reactive oxygen species. This study aimed to assess the impact of zinc oxide nanoparticles (ZnONPs) and selenium oxide nanoparticles (SeONPs) on various sperm functional parameters, including motility, plasma membrane integrity (PMI), mitochondrial membrane potential (MMP), acrosome membrane integrity (ACi), and malondialdehyde (MDA) levels. Methods: Semen samples were collected from 20 Albino Wistar rats. These samples were then divided into six groups: fresh, cryopreservation control, and groups supplemented with SeONPs (1, 2, 5 ㎍/mL) and ZnONPs (0.1, 1, 10 ㎍/mL). Results: Statistical analysis revealed that all concentrations of SeONPs increased total motility and progressive reduction of MDA levels compared to the cryopreservation control group (p<0.05). However, supplementation with ZnONPs did not affect these parameters (p>0.05). Conversely, supplements of 1 and 2 ㎍/mL SeONPs and 1 ㎍/mL ZnONPs contributed to the improvement of PMI and ACi (p<0.05). Yet, no significant change was observed in MMP with any concentration of SeONPs and ZnONPs compared to the cryopreservation control group (p>0.05). Conclusion: The findings suggest that optimal concentrations of SeONPs may enhance sperm parameters during the freezing process.

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References

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