Journal of Periodontal and Implant Science

  • 제54권1호
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  • Pages.13-24
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  • 2024
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  • 2093-2278(pISSN)
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  • 2093-2286(eISSN)
대한치주과학회 (Korean Academy of Periodontology)
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Adjunctive effect of 470-nm and 630-nm light-emitting diode irradiation in experimental periodontitis treatment: a preclinical study

  • Dongseob Lee (Department of Periodontology, School of Dentistry and Dental Research Institute, Seoul National University and Seoul National University Dental Hospital) ;
  • Jungwon Lee (Department of Periodontology, School of Dentistry and Dental Research Institute, Seoul National University and Seoul National University Dental Hospital) ;
  • Sun-Hee Ahn (Medical & Bio Photonics Research Center, Korea Photonics Technology Institute (KOPTI)) ;
  • Woosub Song (Medical & Bio Photonics Research Center, Korea Photonics Technology Institute (KOPTI)) ;
  • Ling Li (Department of Periodontology, School of Dentistry and Dental Research Institute, Seoul National University and Seoul National University Dental Hospital) ;
  • Yang-Jo Seol (Department of Periodontology, School of Dentistry and Dental Research Institute, Seoul National University and Seoul National University Dental Hospital) ;
  • Yong-Moo Lee (Department of Periodontology, School of Dentistry and Dental Research Institute, Seoul National University and Seoul National University Dental Hospital) ;
  • Ki-Tae Koo (Department of Periodontology, School of Dentistry and Dental Research Institute, Seoul National University and Seoul National University Dental Hospital)
  • 투고 : 2022.08.22
  • 심사 : 2023.03.20
  • 발행 : 2024.02.28
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초록

Purpose: This study investigated the adjunctive effect of light-emitting diodes (LEDs) in the treatment of experimental periodontitis. Methods: Experimental periodontitis was induced by placing ligatures around the mandibular second, third, and fourth premolars of 6 beagles for 3 months. After ligature removal, periodontitis progressed spontaneously for 2 months. The animals' hemimandibles were allocated among the following 3 groups: 1) no treatment (control), 2) scaling and root planing (SRP), and 3) SRP with LED irradiation at 470-nm and 630-nm wavelengths (SRP/LED). The probing pocket depth (PPD) and gingival recession (GR) were measured at baseline, 6 weeks, and 12 weeks. The clinical attachment level (CAL) was calculated. After 12 weeks, histological and histomorphometric assessments were performed. The distances from the gingival margin to the apical extent of the junctional epithelium (E) and to the connective tissue (CT) attachment were measured, as was the total length of soft tissue (ST). Results: PPD and CAL increased at 12 weeks compared with baseline in the control group (6.31±0.43 mm to 6.93±0.50 mm, and 6.46±0.60 mm to 7.61±0.78 mm, respectively). PPD and CAL decreased at 12 weeks compared with baseline in the SRP group (6.01±0.59 to 4.81±0.65 mm, and 6.51±0.98 to 5.39±0.93 mm, respectively). PPD and CAL decreased at 12 weeks compared with baseline in the SRP/LED group (6.03±0.39 to 4.46±0.47 mm, and 6.11±0.47 to 4.78±0.57 mm, respectively). The E/ST and CT/ST ratios significantly differed among the 3 groups (P<0.05). The clinical parameters and histologic findings demonstrated that 470-nm and 630-nm wavelength LED irradiation accompanying SRP could improve treatment results. Conclusions: Within the study limitations, 470 nm and 630 nm wavelength LED irradiation might provide additional benefits for periodontitis treatment.

키워드

과제정보

This work was supported by the Development of Advanced Technology for Electronic Systems Program - Optical Convergence for Human Care Technology Development Project (20010763, Development of oral care device with LED standard light source for oral health improvement) funded by the Ministry of Trade, Industry & Energy (MOTIE, Korea).

참고문헌

  1. Darveau RP. Periodontitis: a polymicrobial disruption of host homeostasis. Nat Rev Microbiol 2010;8:481-90. https://doi.org/10.1038/nrmicro2337
  2. Hajishengallis G. Periodontitis: from microbial immune subversion to systemic inflammation. Nat Rev Immunol 2015;15:30-44. https://doi.org/10.1038/nri3785
  3. Ramirez-Mora T, Retana-Lobo C, Valle-Bourrouet G. Biochemical characterization of extracellular polymeric substances from endodontic biofilms. PLoS One 2018;13:e0204081.
  4. Johnston W, Rosier BT, Artacho A, Paterson M, Piela K, Delaney C, et al. Mechanical biofilm disruption causes microbial and immunological shifts in periodontitis patients. Sci Rep 2021;11:9796.
  5. Haffajee AD, Cugini MA, Dibart S, Smith C, Kent RL Jr, Socransky SS. The effect of SRP on the clinical and microbiological parameters of periodontal diseases. J Clin Periodontol 1997;24:324-34. https://doi.org/10.1111/j.1600-051X.1997.tb00765.x
  6. Castelo-Baz P, Ramos-Barbosa I, Martin-Biedma B, Dablanca-Blanco AB, Varela-Patino P, Blanco-Carrion J. Combined endodontic-periodontal treatment of a palatogingival groove. J Endod 2015;41:1918-22. https://doi.org/10.1016/j.joen.2015.08.008
  7. Zhao H, Wang H, Pan Y, Pan C, Jin X. The relationship between root concavities in first premolars and chronic periodontitis. J Periodontal Res 2014;49:213-9. https://doi.org/10.1111/jre.12097
  8. Jepsen K, Jepsen S. Antibiotics/antimicrobials: systemic and local administration in the therapy of mild to moderately advanced periodontitis. Periodontol 2000 2016;71:82-112. https://doi.org/10.1111/prd.12121
  9. Salvi GE, Stahli A, Schmidt JC, Ramseier CA, Sculean A, Walter C. Adjunctive laser or antimicrobial photodynamic therapy to non-surgical mechanical instrumentation in patients with untreated periodontitis: a systematic review and meta-analysis. J Clin Periodontol 2020;47 Suppl 22:176-98. https://doi.org/10.1111/jcpe.13236
  10. Kim S, Kim J, Lim W, Jeon S, Kim O, Koh JT, et al. In vitro bactericidal effects of 625, 525, and 425 nm wavelength (red, green, and blue) light-emitting diode irradiation. Photomed Laser Surg 2013;31:554-62. https://doi.org/10.1089/pho.2012.3343
  11. Kuffler DP. Photobiomodulation in promoting wound healing: a review. Regen Med 2016;11:107-22. https://doi.org/10.2217/rme.15.82
  12. Etemadi A, Sadatmansouri S, Sodeif F, Jalalishirazi F, Chiniforush N. Photobiomodulation effect of different diode wavelengths on the proliferation of human gingival fibroblast cells. Photochem Photobiol 2021;97:1123-8. https://doi.org/10.1111/php.13463
  13. de Freitas LF, Hamblin MR. Proposed mechanisms of photobiomodulation or low-level light therapy. IEEE J Sel Top Quantum Electron 2016;22:7000417.
  14. Mussttaf RA, Jenkins DF, Jha AN. Assessing the impact of low level laser therapy (LLLT) on biological systems: a review. Int J Radiat Biol 2019;95:120-43. https://doi.org/10.1080/09553002.2019.1524944
  15. Wu Y, Zhu T, Yang Y, Gao H, Shu C, Chen Q, et al. Irradiation with red light-emitting diode enhances proliferation and osteogenic differentiation of periodontal ligament stem cells. Lasers Med Sci 2021;36:1535-43. https://doi.org/10.1007/s10103-021-03278-1
  16. Gholami L, Hendi SS, Saidijam M, Mahmoudi R, Tarzemany R, Arkian A, et al. Near-infrared 940- nm diode laser photobiomodulation of inflamed periodontal ligament stem cells. Lasers Med Sci 2022;37:449-59. https://doi.org/10.1007/s10103-021-03282-5
  17. Giannelli M, Lasagni M, Bani D. Photonic therapy in periodontal diseases an overview with appraisal of the literature and reasoned treatment recommendations. Int J Mol Sci 2019;20:4741.
  18. Guffey JS, Wilborn J. In vitro bactericidal effects of 405-nm and 470-nm blue light. Photomed Laser Surg 2006;24:684-8. https://doi.org/10.1089/pho.2006.24.684
  19. Chaweewannakorn C, Santiwong P, Surarit R, Sritanaudomchai H, Chintavalakorn R. The effect of LED photobiomodulation on the proliferation and osteoblastic differentiation of periodontal ligament stem cells: in vitro. J World Fed Orthod 2021;10:79-85. https://doi.org/10.1016/j.ejwf.2021.03.003
  20. Avci P, Gupta A, Sadasivam M, Vecchio D, Pam Z, Pam N, et al. Low-level laser (light) therapy (LLLT) in skin: stimulating, healing, restoring. Semin Cutan Med Surg 2013;32:41-52.
  21. Lee J, Song HY, Ahn SH, Song W, Seol YJ, Lee YM, et al. In vitro investigation of the antibacterial and anti-inflammatory effects of LED irradiation. J Periodontal Implant Sci 2023;53:110-9. https://doi.org/10.5051/jpis.2200920046
  22. Percie du Sert N, Ahluwalia A, Alam S, Avey MT, Baker M, Browne WJ, et al. Reporting animal research: explanation and elaboration for the ARRIVE guidelines 2.0. PLoS Biol 2020;18:e3000411.
  23. Basso FG, Soares DG, Pansani TN, Cardoso LM, Scheffel DL, de Souza Costa CA, et al. Proliferation, migration, and expression of oral-mucosal-healing-related genes by oral fibroblasts receiving low-level laser therapy after inflammatory cytokines challenge. Lasers Surg Med 2016;48:1006-14. https://doi.org/10.1002/lsm.22553
  24. Theodoro LH, Garcia VG, Haypek P, Zezell DM, Eduardo CP. Morphologic analysis, by means of scanning electron microscopy, of the effect of Er: YAG laser on root surfaces submitted to scaling and root planing. Pesqui Odontol Bras 2002;16:308-12. https://doi.org/10.1590/S1517-74912002000400005
  25. Franco EJ, Greghi SLA, de Assis GF. Comparative evaluation of periodontal radicular therapies-conventional and er: yag laser-based on rat connective tissue response. Oral Sci 2005;1:35-42.
  26. Atieh MA. Photodynamic therapy as an adjunctive treatment for chronic periodontitis: a meta-analysis. Lasers Med Sci 2010;25:605-13. https://doi.org/10.1007/s10103-009-0744-6
  27. Giannelli M, Materassi F, Fossi T, Lorenzini L, Bani D. Treatment of severe periodontitis with a laser and light-emitting diode (LED) procedure adjunctive to scaling and root planing: a double-blind, randomized, single-center, split-mouth clinical trial investigating its efficacy and patient-reported outcomes at 1 year. Lasers Med Sci 2018;33:991-1002. https://doi.org/10.1007/s10103-018-2441-9
  28. Wu J, Chu Z, Ruan Z, Wang X, Dai T, Hu X. Changes of intracellular porphyrin, reactive oxygen species, and fatty acids profiles during inactivation of methicillin-resistant Staphylococcus aureus by antimicrobial blue light. Front Physiol 2018;9:1658.
  29. Van Tran V, Chae M, Moon JY, Lee YC. Light emitting diodes technology-based photobiomodulation therapy (PBMT) for dermatology and aesthetics: Recent applications, challenges, and perspectives. Opt Laser Technol 2021;135:106698.
  30. Kim SK, You HR, Kim SH, Yun SJ, Lee SC, Lee JB. Skin photorejuvenation effects of light-emitting diodes (LEDs): a comparative study of yellow and red LEDs in vitro and in vivo. Clin Exp Dermatol 2016;41:798-805. https://doi.org/10.1111/ced.12902
  31. Mamalis A, Jagdeo J. Light-emitting diode-generated red light inhibits keloid fibroblast proliferation. Dermatol Surg 2015;41:35-9. https://doi.org/10.1097/01.DSS.0000452650.06765.51
  32. Waerhaug J. Healing of the dento-epithelial junction following subgingival plaque control. II: As observed on extracted teeth. J Periodontol 1978;49:119-34. https://doi.org/10.1902/jop.1978.49.3.119
  33. Wilson TG Jr, Carnio J, Schenk R, Myers G. Absence of histologic signs of chronic inflammation following closed subgingival scaling and root planing using the dental endoscope: human biopsies - a pilot study. J Periodontol 2008;79:2036-41. https://doi.org/10.1902/jop.2008.080190
  34. Schroeder H, Listgarten MA. The junctional epithelium: origin, structure and significance. Monogr Dev Biol 1971;2:1-134.
  35. Tsukamoto Y, Usui M, Yamamoto G, Takagi Y, Tachikawa T, Yamamoto M, et al. Role of the junctional epithelium in periodontal innate defense and homeostasis. J Periodontal Res 2012;47:750-7. https://doi.org/10.1111/j.1600-0765.2012.01490.x
  36. Listgarten MA. Periodontal probing: what does it mean? J Clin Periodontol 1980;7:165-76. https://doi.org/10.1111/j.1600-051X.1980.tb01960.x
  37. Moreira AL, Novaes AB Jr, Grisi MF, Taba M Jr, Souza SL, Palioto DB, et al. Antimicrobial photodynamic therapy as an adjunct to non-surgical treatment of aggressive periodontitis: a split-mouth randomized controlled trial. J Periodontol 2015;86:376-86. https://doi.org/10.1902/jop.2014.140392
  38. Betsy J, Prasanth CS, Baiju KV, Prasanthila J, Subhash N. Efficacy of antimicrobial photodynamic therapy in the management of chronic periodontitis: a randomized controlled clinical trial. J Clin Periodontol 2014;41:573-81. https://doi.org/10.1111/jcpe.12249