Restorative Dentistry and Endodontics

The Korean Academy of Conservative Dentistry (대한치과보존학회)
권호 표지
DOI QR코드

DOI QR Code

Pomegranate extract on eroded dentin: antioxidant action, bond strength and morphology of the adhesive interface after aging

  • Received : 2023.06.20
  • Accepted : 2023.12.30
  • Published : 2024.02.28
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives: This study aimed to evaluate the effect of pomegranate solution (Punica granatum) on eroded dentin through antioxidant action, shear bond strength (SBS) and interface morphology. Materials and Methods: The 10% pomegranate peel extract was prepared by the lyophilization method. Punicalagin polyphenol was confirmed by high-performance liquid chromatography. Antioxidant activity was evaluated by capturing the 2,2-diphenyl1-picrylhydrazyl (DPPH) radical. For the SBS, 48 dentin fragments were divided into sound or eroded, and subdivided according to the pretreatment (n = 12): water or P. granatum. The surfaces were restored with self-etch adhesive and a bulk-fill resin (Ecosite; DMG). The SBS was done immediately (24 hours) and after thermal cycling + water storage (12 months). For scanning electron microscopy, 48 dentin fragments (24 sound and 24 eroded) received the same treatments as for SBS (n = 6), and they were analyzed after 24 hours and 12 months. Results: The P. granatum had antioxidant action similar (p = 0.246) to the phenolic standard antioxidants. After 24 hours, eroded dentin had lower SBS than sound dentin (p < 0.001), regardless of the pretreatment. After 12 months, P. granatum maintained the SBS of sound dentin (13.46 ± 3.42 MPa) and eroded dentin (10.96 ± 1.90 MPa) statistically similar. The lowest values were found on eroded dentin treated with water (5.75 ± 1.65 MPa) (p < 0.001). P. granatum on eroded dentin caused peritubular demineralization and hybrid layer with resin tags. Conclusions: The pomegranate extract had antioxidant action and preserved the adhesive interface of the eroded dentin.

Keywords

Acknowledgement

The authors are thankful to the financial support of the National Council for Scientific and Technological Development (CNPq # 140877/2019-3). We would also like to acknowledge the DMG Dental Company (Germany) for providing the resin and adhesive system used in this research.

References

  1. Chan AS, Tran TTK, Hsu YH, Liu SYS, Kroon J. A systematic review of dietary acids and habits on dental erosion in adolescents. Int J Paediatr Dent 2020;30:713-733. https://doi.org/10.1111/ipd.12643
  2. Methuen M, Kangasmaa H, Alaraudanjoki VK, Suominen AL, Anttonen V, Vahanikkila H, et al. Prevalence of erosive tooth wear and associated dietary factors among a group of Finnish adolescents. Caries Res 2022;56:477-487. https://doi.org/10.1159/000527305
  3. Tulek A, Mulic A, Refsholt Stenhagen K, Galtung HK, Saeed M, Utheim TP, et al. Dental erosion in mice with impaired salivary gland function. Acta Odontol Scand 2020;78:390-400. https://doi.org/10.1080/00016357.2020.1734234
  4. Kawar N, Park SG, Schwartz JL, Callahan N, Obrez A, Yang B, et al. Salivary microbiome with gastroesophageal reflux disease and treatment. Sci Rep 2021;11:188-196. https://doi.org/10.1038/s41598-020-80170-y
  5. Wiegand A, Lechte C, Kanzow P. Adhesion to eroded enamel and dentin: systematic review and metaanalysis. Dent Mater 2021;37:1845-1853. https://doi.org/10.1016/j.dental.2021.09.014
  6. Frassetto A, Breschi L, Turco G, Marchesi G, Di Lenarda R, Tay FR, et al. Mechanisms of degradation of the hybrid layer in adhesive dentistry and therapeutic agents to improve bond durability--a literature review. Dent Mater 2016;32:e41-e53. https://doi.org/10.1016/j.dental.2015.11.007
  7. Kanniappan G, Hari P, Jujare RH. Comparative evaluation of resin dentin interface using universal and total- etch adhesive systems on sound and eroded dentin: in vitro study. Eur J Dent 2022;16:153-160.  https://doi.org/10.1055/s-0041-1734469
  8. Belmar da Costa M, Delgado AHS, Pinheiro de Melo T, Amorim T, Mano Azul A. Analysis of laboratory adhesion studies in eroded enamel and dentin: a scoping review. Biomater Investig Dent 2021;8:24-38.  https://doi.org/10.1080/26415275.2021.1884558
  9. Shen J, Xie H, Wang Q, Wu X, Yang J, Chen C. Evaluation of the interaction of chlorhexidine and MDP and its effects on the durability of dentin bonding. Dent Mater 2020;36:1624-1634. https://doi.org/10.1016/j.dental.2020.10.006
  10. Siqueira FSF, Cardenas AM, Ocampo JB, Hass V, Bandeca MC, Gomes JC, et al. Bonding performance of universal adhesives to eroded dentin. J Adhes Dent 2018;20:121-132.
  11. Jacobsen JA, Fullagar JL, Miller MT, Cohen SM. Identifying chelators for metalloprotein inhibitors using a fragment-based approach. J Med Chem 2011;54:591-602. https://doi.org/10.1021/jm101266s
  12. Grimm T, Schafer A, Hogger P. Antioxidant activity and inhibition of matrix metalloproteinases by metabolites of maritime pine bark extract (pycnogenol). Free Radic Biol Med 2004;36:811-822. https://doi.org/10.1016/j.freeradbiomed.2003.12.017
  13. Suratno IR, Dwiandhono I, Purnama RB. Pomegranate (Punica granatum L.) gel extract as an antioxidant on the shear bond strength of a resin composite post-bleaching application with 40% hydrogen peroxide. Dent Jour (Gigi) 2021;54:87-91.
  14. Hanafy SM, Abd El-Shafea YM, Saleh WD, Fathy HM. Chemical profiling, in vitro antimicrobial and antioxidant activities of pomegranate, orange and banana peel-extracts against pathogenic microorganisms. J Genet Eng Biotechnol 2021;19:80-90. https://doi.org/10.1186/s43141-021-00151-0
  15. Baena E, Cunha SR, Maravic T, Comba A, Paganelli F, Alessandri-Bonetti G, et al. Effect of chitosan as a cross-linker on matrix metalloproteinase activity and bond stability with different adhesive systems. Mar Drugs 2020;18:263.
  16. Fakhri E, Eslami H, Maroufi P, Pakdel F, Taghizadeh S, Ganbarov K, et al. Chitosan biomaterials application in dentistry. Int J Biol Macromol 2020;162:956-974. https://doi.org/10.1016/j.ijbiomac.2020.06.211
  17. Ziotti IR, Palma-Dibb RG, Cornona SAM, Souza-Gabriel AE. Viability of using natural extracts in dental restorative treatment. Eur J Pharm Med Res 2016;3:53-61.
  18. Peng SY, Hsiao CC, Lan TH, Yen CY, Farooqi AA, Cheng CM, et al. Pomegranate extract inhibits migration and invasion of oral cancer cells by downregulating matrix metalloproteinase-2/9 and epithelial-mesenchymal transition. Environ Toxicol 2020;35:673-682. https://doi.org/10.1002/tox.22903
  19. Ghavipour M, Sotoudeh G, Tavakoli E, Mowla K, Hasanzadeh J, Mazloom Z. Pomegranate extract alleviates disease activity and some blood biomarkers of inflammation and oxidative stress in rheumatoid arthritis patients. Eur J Clin Nutr 2017;71:92-96. https://doi.org/10.1038/ejcn.2016.151
  20. Chau TP, Veeraragavan GR, Narayanan M, Chinnathambi A, Alharbi SA, Subramani B, et al. Green synthesis of zirconium nanoparticles using Punica granatum (pomegranate) peel extract and their antimicrobial and antioxidant potency. Environ Res 2022;209:112771.
  21. Eghbali S, Askari SF, Avan R, Sahebkar A. Therapeutic effects of Punica granatum (pomegranate): an updated review of clinical trials. J Nutr Metab 2021;2021:5297162.
  22. Shaygannia E, Bahmani M, Zamanzad B, Rafieian-Kopaei M. A review study on Punica granatum L. J Evid Based Complementary Altern Med 2016;21:221-227. https://doi.org/10.1177/2156587215598039
  23. BenSaad LA, Kim KH, Quah CC, Kim WR, Shahimi M. Anti-inflammatory potential of ellagic acid, gallic acid and punicalagin A&B isolated from Punica granatum. BMC Complement Altern Med 2017;17:47. 
  24. Hosseini A, Razavi BM, Hosseinzadeh H. Protective effects of pomegranate (Punica granatum) and its main components against natural and chemical toxic agents: a comprehensive review. Phytomedicine 2023;109:154581.
  25. Danesi F, Ferguson LR. Could pomegranate juice help in the control of inflammatory diseases? Nutrients 2017;9:1-23. https://doi.org/10.3390/nu9090958
  26. Durazzo A, Lucarini M, Souto EB, Cicala C, Caiazzo E, Izzo AA, et al. Polyphenols: a concise overview on the chemistry, occurrence, and human health. Phytother Res 2019;33:2221-2243. https://doi.org/10.1002/ptr.6419
  27. Abodahb SM, Nizay MA, ElKorashy ME. Effect of pomegranate and banana extracts on microshear bond strength of resin composite to bleached bovine enamel: an in vitro study. Alexandria Dent J 2022;9:251-263. https://doi.org/10.21608/adjg.2022.103255.1430
  28. Souza-Gabriel AE, Sousa-Neto MD, Scatolin RS, Corona SAM. Durability of resin on bleached dentin treated with antioxidant solutions or lasers. J Mech Behav Biomed Mater 2020;104:103647.
  29. Sajana EM, Akhil YB, Lekshmy SD, Sreeja S, Lakshmi A. Effect of pomegranate peel extract on the shear bond strength of composite resin to dentin using etch& rinse adhesive system. J Med Sci Clin Res 2020;08:123-129.
  30. Peyro Mousavi SF, Ganjovi A, Eskandarizadeh A, Saidi AR, Isaei E. Evaluating the antibacterial effect of synthesized herbal toothpastes and their efficacy for dentine tubule occlusion: scanning electron microscopy and energy-dispersive X-ray spectroscopy analysis. Microsc Res Tech 2022;85:19-27. https://doi.org/10.1002/jemt.23881
  31. Nirmal GD, Sai Sankar AJ, Sridevi E, Sridhar M, Sankar KS, Satish PR. Comparative evaluation of chelating efficacy of nano-chitosan, pomegranate extract, and ethylenediaminetetraacetic acid on primary radicular dentin: an in vitro study. J Indian Soc Pedod Prev Dent 2022;40:201-207. https://doi.org/10.4103/jisppd.jisppd_231_21
  32. Sharafeddin F, Farshad F. The effect of aloe vera, pomegranate peel, grape seed extract, green tea, and sodium ascorbate as antioxidants on the shear bond strength of composite resin to home-bleached enamel. J Dent (Shiraz) 2015;16:296-301.
  33. Sarialioglu Gungor A, Donmez N. Dentin erosion preventive effects of various plant extracts: an in vitro atomic force microscopy, scanning electron microscopy, and nanoindentation study. Microsc Res Tech 2021;84:1042-1052. https://doi.org/10.1002/jemt.23665
  34. Gallas JA, Pelozo LL, Oliveira WP, Salvador SL, Corona SM, Souza-Gabriel AE. Characterization, antimicrobial activity, and antioxidant efficacy of a pomegranate peel solution against persistent root canal pathogens. Cureus 2023;15:e43142.
  35. Ururahy MS, Curylofo-Zotti FA, Galo R, Nogueira LF, Ramos AP, Corona SA. Wettability and surface morphology of eroded dentin treated with chitosan. Arch Oral Biol 2017;75:68-73. https://doi.org/10.1016/j.archoralbio.2016.11.017
  36. Mortensen D, Mulic A, Pallesen U, Twetman S. Awareness, knowledge and treatment decisions for erosive tooth wear: a case-based questionnaire among Danish dentists. Clin Exp Dent Res 2021;7:56-62. https://doi.org/10.1002/cre2.339
  37. Milosevic A, Burnside G. The survival of direct composite restorations in the management of severe tooth wear including attrition and erosion: A prospective 8-year study. J Dent 2016;44:13-19. https://doi.org/10.1016/j.jdent.2015.10.015
  38. Favetti M, Schroeder T, Montagner AF, Correa MB, Pereira-Cenci T, Cenci MS. Effectiveness of pretreatment with chlorhexidine in restoration retention: a 36-month follow-up randomized clinical trial. J Dent 2017;60:44-49. https://doi.org/10.1016/j.jdent.2017.02.014
  39. Rodriguez ES, Julio LM, Henning C, Diehl BW, Tomas MC, Ixtaina VY. Effect of natural antioxidants on the physicochemical properties and stability of freeze-dried microencapsulated chia seed oil. J Sci Food Agric 2019;99:1682-1690. https://doi.org/10.1002/jsfa.9355
  40. Akhtar S, Ismail T, Fraternale D, Sestili P. Pomegranate peel and peel extracts: chemistry and dietary characteristics. Food Chem 2015;174:417-425. https://doi.org/10.1016/j.foodchem.2014.11.035
  41. Russo M, Fanali C, Tripodo G, Dugo P, Muleo R, Dugo L, et al. Analysis of phenolic compounds in different parts of pomegranate (Punica granatum) fruit by HPLC-PDA-ESI/MS and evaluation of their antioxidant activity: application to different Italian varieties. Anal Bioanal Chem 2018;410:3507-3520.  https://doi.org/10.1007/s00216-018-0854-8
  42. Salwe KJ, Sachdev DO, Bahurupi Y, Kumarappan M. Evaluation of antidiabetic, hypolipedimic and antioxidant activity of hydroalcoholic extract of leaves and fruit peel of Punica granatum in male Wistar albino rats. J Nat Sci Biol Med 2015;6:56-62. https://doi.org/10.4103/0976-9668.149085
  43. Prior RL, Cao G. In vivo total antioxidant capacity: comparison of different analytical methods. Free Radic Biol Med 1999;27:1173-1181. https://doi.org/10.1016/S0891-5849(99)00203-8
  44. Lee J, Sung JM, Cho HJ, Woo SH, Kang MC, Yong HI, et al. Natural extracts as inhibitors of microorganisms and lipid oxidation in emulsion sausage during storage. Food Sci Anim Resour 2021;41:1060-1077. https://doi.org/10.5851/kosfa.2021.e58
  45. Jalali A, Kiafar M, Seddigh M, Zarshenas MM. Punica granatum as a source of natural antioxidant and antimicrobial agent: a comprehensive review on related investigations. Curr Drug Discov Technol 2021;18:207-224. https://doi.org/10.2174/1570163817666200430001822
  46. Zhao X, Yuan Z. Anthocyanins from pomegranate (Punica granatum L.) and their role in antioxidant capacities in vitro. Chem Biodivers 2021;18:e2100399.
  47. Paik Y, Kim JH, Yoo KH, Yoon SY, Kim YI. Dentin biomodification with flavonoids and calcium phosphate ion clusters to improve dentin bonding stability. Materials (Basel) 2022;15:1494-1507. https://doi.org/10.3390/ma15041494
  48. Frattes FC, Augusto MG, Torres CRG, Pucci CR, Borges AB. Bond strength to eroded enamel and dentin using a universal adhesive system. J Adhes Dent 2017;19:121-127.
  49. Fang M, Liu R, Xiao Y, Li F, Wang D, Hou R, et al. Biomodification to dentin by a natural crosslinker improved the resin-dentin bonds. J Dent 2012;40:458-466. https://doi.org/10.1016/j.jdent.2012.02.008
  50. Paken G, Comlekoglu ME, Sonugelen M. Detection of the hybrid layer biodegradation initiation factor with a scanning electron microscope. Microsc Res Tech 2021;84:2166-2175. https://doi.org/10.1002/jemt.23771
  51. Giacomini C, Maurina R, Tomazoni F, Bellan MC, Alessandretti R, Galafassi D. Bond strength of restorations made using universal adhesive systems in teeth subjected to acid erosion. JOI 2019;8:7-17. https://doi.org/10.18256/2238-510X.2019.v8i1.3067