Restorative Dentistry and Endodontics

The Korean Academy of Conservative Dentistry (대한치과보존학회)
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Effect of different storage media on elemental analysis and microhardness of cervical cavity margins restored with a bioactive material

  • Hoda Saleh Ismail (Conservative Dentistry Department, Faculty of Dentistry, Mansoura University) ;
  • Brian Ray Morrow (Department of Bioscience Research, College of Dentistry, University of Tennessee Health Science Center) ;
  • Ashraf Ibrahim Ali (Conservative Dentistry Department, Faculty of Dentistry, Mansoura University) ;
  • Rabab Elsayed Elaraby Mehesen (Conservative Dentistry Department, Faculty of Dentistry, Mansoura University) ;
  • Salah Hasab Mahmoud (Conservative Dentistry Department, Faculty of Dentistry, Mansoura University) ;
  • Franklin Garcia-Godoy (Department of Bioscience Research, College of Dentistry, University of Tennessee Health Science Center)
  • Received : 2022.05.29
  • Accepted : 2023.01.12
  • Published : 2024.02.28
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Abstract

Objectives: This study aimed to investigate the elemental analysis and microhardness of a bioactive material (Activa) and marginal tooth structure after storage in different media. Materials and Methods: Fifteen teeth received cervical restorations with occlusal enamel and gingival dentin margins using the tested material bonded with a universal adhesive, 5 of them on the 4 axial surfaces and the other 10 on only the 2 proximal surfaces. The first 5 teeth were sectioned into 4 restorations each, then stored in 4 different media; deionized water, Dulbecco's phosphate buffered saline (DPBS), Tris buffer, and saliva. The storage period for deionized water was 24 hours while it was 3 months for the other media. Each part was analyzed by scanning electron microscopy-energy dispersive spectroscopy (SEM-EDS) analysis for different substrates/distances and the wt% of calcium, phosphorus, silica, and fluoride were calculated. The other 10 teeth were sectioned across the restoration, stored in either Tris buffer or saliva for 24 hours or 3 months, and were evaluated for microhardness of different substrates/areas. Data were analyzed using analysis of variance and Tukey's post hoc test. Results: Enamel and dentin interfaces in the DPBS group exhibited a significant increase in calcium and phosphorus wt%. Both silica and fluoride significantly increased in tooth structure up to a distance of 75 ㎛ in the 3-month-media groups than the immediate group. Storage media did not affect the microhardness values. Conclusions: SEM-EDS analysis suggests an ion movement between Activa and tooth structure through a universal adhesive while stored in DPBS.

Keywords

Acknowledgement

The authors acknowledge the UTHSC Office of Scientific Writing for editing the manuscript.

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