Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Hepatoprotective Effects of the Extracts of Alnus japonica Leaf on Alcohol-Induced Liver Damage in HepG2/2E1 Cells

알코올로 유도된 간손상 모델 HepG2/2E1 세포에서 오리나무 잎 추출물의 간보호효과

  • Bo-Ram Kim (Honam National Institute of Biological Resources) ;
  • Tae-Su Kim (Honam National Institute of Biological Resources) ;
  • Su Hui Seong (Honam National Institute of Biological Resources) ;
  • Seahee Han (Honam National Institute of Biological Resources) ;
  • Jin-Ho Kim (Honam National Institute of Biological Resources) ;
  • Chan Seo (Honam National Institute of Biological Resources) ;
  • Ha-Nul Lee (Honam National Institute of Biological Resources) ;
  • Sua Im (Honam National Institute of Biological Resources) ;
  • Jung Eun Kim (Honam National Institute of Biological Resources) ;
  • Ji Min Jung (Honam National Institute of Biological Resources) ;
  • Do-Yun Jeong (Honam National Institute of Biological Resources) ;
  • Kyung-Min Choi (Honam National Institute of Biological Resources) ;
  • Jin-Woo Jeong (Honam National Institute of Biological Resources)
  • 김보람 (국립호남권생물자원관) ;
  • 김태수 (국립호남권생물자원관) ;
  • 성수희 (국립호남권생물자원관) ;
  • 한세희 (국립호남권생물자원관) ;
  • 김진호 (국립호남권생물자원관) ;
  • 서찬 (국립호남권생물자원관) ;
  • 이하늘 (국립호남권생물자원관) ;
  • 임수아 (국립호남권생물자원관) ;
  • 김정은 (국립호남권생물자원관) ;
  • 정지민 (국립호남권생물자원관) ;
  • 정도윤 (국립호남권생물자원관) ;
  • 최경민 (국립호남권생물자원관) ;
  • 정진우 (국립호남권생물자원관)
  • Received : 2024.02.20
  • Accepted : 2024.03.20
  • Published : 2024.04.01
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Abstract

Alcoholic liver disease (ALD) is a significant risk factor in the global disease burden. The stem bark of the Betulaceae plant Alnus japonica, which is indigenous to Korea, has been used as a popular folk medicine for hepatitis and cancer. However, the preventive effect of Alnus japonica leaf extracts on alcohol-related liver damage has not been investigated. The objective of this study was to investigate the hepatoprotective effects of the extracts of Alnus japonica leaf (AJL) against ethanol-induced liver damage in HepG2/2E1 cells. Treatment with AJL significantly prevented ethanol-induced cytotoxicity in HepG2/2E1 cells by reducing the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST). This protective effect was likely associated with antioxidant potential of AJL, as evidenced by the attenuation of reactive oxygen species (ROS) and malondialdehyde (MDA) production and restoration of the depleted glutathione (GSH) levels in ethanol-induced HepG2/2E1 cells. Our findings suggest that FCC might be considered as a useful agent in the prevention of liver damage induced by oxidative stress by increasing the antioxidant defense mechanism.

활성산소종은 미토콘드리아 대사활동을 통해 생성되는 반응성이 강한 산소 함유 분자로서 특히 간에서 복잡한 세포 분자와 반응하여 산화적 스트레스를 유발하는 물질이다(Choi et al., 2012). 에탄올 대사 과정에서는 ROS가 생성되어 산화스트레스를 유발하며, 과도한 에탄올은 ROS를 제거하는 항산화 시스템을 억제함으로써 지방, 단백질 또는 DNA와 같은 생체 분자에 산화 손상을 유발하여 세포를 손상시킨다(Albano, 2006; Koch et al., 2004). 본 연구에서는 오리나무 잎 추출물(AJL)에서 알코올(EtOH)로 유발된 산화적 스트레스에 대한 간세포 손상의 보호 효과를 평가하기 위해 HepG2/2E1 세포에서 EtOH와 AJL 및 대조군silymarin을 각각 처리한 후 세포생존율을 평가하고 세포독성이 없는 범위를 설정하여 활성산소종 생성량, 항산화 관련 지표(DPPH, ABTS, MDA, GSH) 및 간 손상 지표(AST 및 ALT)를 측정하였다. 알코올에 대한 간 보호 효과에서는 알코올만 처리한 군의 세포생존율이 53.8%로 감소하였으나 AJL을 함께 처리 군에서 세포생존율이 85.1%의 유의적인 증가로 간 보호효과를 확인하였다. 다음으로 AJL에서 세포 내 ROS 생성 억제능은 에탄올 처리군에서 정상군에 비해 유의적으로 ROS 생성이 증가하였으나 AJL 농도별 처리시 정상군 수준으로 ROS 생성을 현저하게 감소시켰다. MDA 함량은 에탄올 처리군에서 무처리군에 비해 유의적으로 증가하였으나 AJL 처리시 농도 의존적으로 에탄올 처리군에 비해 유의적인 차이를 보이며 MDA 함량을 감소시켰다. GSH 함량은 에탄올 처리군에서 정상군보다 유의적으로 함량이 감소하였으나 AJL 농도별 처리군 모두 에탄올 처리군에 비해 GSH 함량을 증가시켰다. 또한 간손상 지표로 활용되는 AST 및 ALT 활성 변화 확인 시, 에탄올 처리에 따른 간세포 손상군에 비하여 AJL 처리군에서는 농도 의존적으로 현저하게 감소시켰으며, 대조군silymarin과 비교하여 유사한 감소된 결과를 관찰하였다. 이상의 결과로부터 종합해 볼때AJL은 간손상에 대한 보호 및 간손상 억제 가능성을 기대할 수 있으며, 간기능 개선 효과에 대한 건강기능식품개발의 기초자료로서 활용될 수 있을 것이라 사료된다.

Keywords

Acknowledgement

본 결과물은 환경부의 재원으로 국립호남권생물자원관(HNIBR202302115) 및 한국환경산업기술원의 다부처 국가생명연구자원 선진화 사업(RS-2023-00230402)을 지원받아 수행된 연구이며, 이에 감사드립니다.

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