International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Isolation and identification of insect pathogenic fungus from silkworms with suspected white muscardine disease

  • Seul Ki Park (Department of Agricultural Biology, National Institute of Agricultural Sciences, RDA) ;
  • Chan Young Jeong (Department of Agricultural Biology, National Institute of Agricultural Sciences, RDA) ;
  • Hyeok Gyu Kwon (Department of Agricultural Biology, National Institute of Agricultural Sciences, RDA) ;
  • Ji Hae Lee (Department of Agricultural Biology, National Institute of Agricultural Sciences, RDA) ;
  • Sang Kuk Kang (Department of Agricultural Biology, National Institute of Agricultural Sciences, RDA) ;
  • Seong-Wan Kim (Department of Agricultural Biology, National Institute of Agricultural Sciences, RDA) ;
  • Seong-Ryul Kim (Department of Agricultural Biology, National Institute of Agricultural Sciences, RDA) ;
  • Jong Woo Park (Department of Agricultural Biology, National Institute of Agricultural Sciences, RDA)
  • Received : 2023.11.22
  • Accepted : 2024.01.09
  • Published : 2024.03.31
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Abstract

The value of silkworms as functional health food materials has increased, as has the interest in its disease control for stable production, and in the economic value of entomopathogenic microorganisms. In this study, we isolated and identified disease-causing fungi from white muscardine silkworms, and confirmed whether this strain could produce white muscardine silkworms. For the analysis of the cause of white muscardine disease in the infected silkworms, the fungi and prokaryotes causing the disease were identified, isolated, and identified using metagenome analysis. Metagenomic analysis detected a large amount of the fungus Metarhizium rileyi in silkworms, and a large amount of the bacterium Enterococcus mundtii, which was presumed to be the causative agent of the disease. For accurate identification of the fungi, these were purified by culture medium, and sequencing and phylogenetic tree analyses were performed using an internal transcribed spacer. As a result, M. rileyi, Cladosporium cladosporioides, and C. tenuissimum were identified. In general, M. rileyi is known to form green conidia, but in this study, white-yellow conidia were formed, indicating that the exact causative agent of the fungal disease cannot be estimated by diagnosing the symptoms. Thus, a diagnostic method is necessary for the continuously collection of required pathogens, and identifying their morphological and genetic characteristics.

Keywords

Acknowledgement

This study was supported by the 2023 RDA Fellowship Program of the National Institute of Agricultural Science and a grant (No. PJ01001805) of the Rural Development Administration of the Republic of Korea.

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