Biomedical Science Letters (대한의생명과학회지)

The Korean Society for Biomedical Laboratory Sciences (대한의생명과학회)
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Protective Effects of BCC Against Oxidative Stress in Cardiomyocyte Cells

  • Received : 2023.12.07
  • Accepted : 2024.03.08
  • Published : 2024.03.31
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Abstract

Oxidative stress caused by elevated reactive oxygen species (ROS) in the heart causes various heart diseases. Oxidative stress is known as a factor that causes diseases in various organs as well as the heart. Diseases such as heart failure, myocardial infarction, and cardiomyopathy caused by oxidative stress in the heart can be treated with medication or surgery. Recently, blood cells concentrate (BCC) is used in various treatment areas such as orthopedics, gynecology, and urology. BCC therapy is applied to treatment by concentrating platelets and white blood cells necessary for regeneration through simple centrifugation using autologous blood. As the platelets are activated, many growth factors are released from alpha granules of the platelets. Growth factors such as TGF-β1, PDGF, VEGF, and EGF derived from platelets are involved in various cell signaling pathway. Due to these growth factors, BCC can contribute to tissue regeneration and can treat various diseases. CD34+ cells contained in BCC may also play an important role in tissue regeneration. In this study, we investigated whether BCC has a regenerative effect on heart disease, and if so, what mechanism causes the effect. To observe this, cardiomyocyte cells were treated with H2O2 to induce oxidative stress. And the effect was confirmed in the presence or absence of BCC. As a result, in the presence of BCC, the oxidative stress of cardiomyocyte cells was reduced and cell damage was also reduced. These results suggest that BCC therapy can be a new treatment alternative for heart disease.

Keywords

Acknowledgement

The authors thank Hyun-Seung Kim of MODELINE CLINIC for his assistance with blood and BCC preparation.

References

  1. Adeoye O, Olawumi J, Opeyemi A, Christiania O. Review on the role of glutathione on oxidative stress and infertility. JBRA Assist Reprod. 2018. 22: 61-66. 
  2. Apel K, Hirt H. Reactive oxygen species: Metabolism, oxidative stress, and signal transduction. Annu Rev Plant Biol. 2004. 55: 373-399.  https://doi.org/10.1146/annurev.arplant.55.031903.141701
  3. Bader R, Ibrahim JN, Moussa M, Mourad A, Azoury J, Azoury J, Alaaeddine N. In vitro effect of autologous platelet-rich plasma on h(2) o(2)-induced oxidative stress in human spermatozoa. Andrology. 2020. 8: 191-200.  https://doi.org/10.1111/andr.12648
  4. Burchard R, Huflage H, Soost C, Richter O, Bouillon B, Graw JA. Efficiency of platelet-rich plasma therapy in knee osteoarthritis does not depend on level of cartilage damage. J Orthop Surg Res. 2019. 14: 153. 
  5. Chistiakov DA, Shkurat TP, Melnichenko AA, Grechko AV, Orekhov AN. The role of mitochondrial dysfunction in cardiovascular disease: A brief review. Ann Med. 2018. 50: 121-127.  https://doi.org/10.1080/07853890.2017.1417631
  6. D'Oria R, Schipani R, Leonardini A, Natalicchio A, Perrini S, Cignarelli A, Laviola L, Giorgino F. The role of oxidative stress in cardiac disease: From physiological response to injury factor. Oxid Med Cell Longev. 2020. 2020: 5732956. 
  7. Deshmukh NS, Belgaumkar VA. Platelet-rich plasma augments subcision in atrophic acne scars: A split-face comparative study. Dermatol Surg. 2019. 45: 90-98.  https://doi.org/10.1097/DSS.0000000000001614
  8. Dhurat R, Sukesh M. Principles and methods of preparation of platelet-rich plasma: A review and author's perspective. J Cutan Aesthet Surg. 2014. 7: 189-197.  https://doi.org/10.4103/0974-2077.150734
  9. Etulain J, Mena HA, Meiss RP, Frechtel G, Gutt S, Negrotto S, Schattner M. An optimised protocol for platelet-rich plasma preparation to improve its angiogenic and regenerative properties. Sci Rep. 2018. 8: 1513. 
  10. Gkini MA, Kouskoukis AE, Tripsianis G, Rigopoulos D, Kouskoukis K. Study of platelet-rich plasma injections in the treatment of androgenetic alopecia through an one-year period. J Cutan Aesthet Surg. 2014. 7: 213-219. 
  11. Gutierrez CM, Lopez C, Giraldo CE, Carmona JU. Study of a two-step centrifugation protocol for concentrating cells and growth factors in bovine platelet-rich plasma. Vet Med Int. 2017. 2017: 1950401. 
  12. Hargrave B, Li F. Nanosecond pulse electric field activation of platelet-rich plasma reduces myocardial infarct size and improves left ventricular mechanical function in the rabbit heart. J Extra Corpor Technol. 2012. 44: 198-204.  https://doi.org/10.1051/ject/201244198
  13. Kreuz PC, Kruger JP, Metzlaff S, Freymann U, Endres M, Pruss A, Petersen W, Kaps C. Platelet-rich plasma preparation types show impact on chondrogenic differentiation, migration, and proliferation of human subchondral mesenchymal progenitor cells. Arthroscopy. 2015. 31: 1951-1961.  https://doi.org/10.1016/j.arthro.2015.03.033
  14. Marck RE, Gardien KLM, Vlig M, Breederveld RS, Middelkoop E. Growth factor quantification of platelet-rich plasma in burn patients compared to matched healthy volunteers. Int J Mol Sci. 2019. 20. 
  15. Muller P, Lemcke H, David R. Stem cell therapy in heart diseases - cell types, mechanisms and improvement strategies. Cell Physiol Biochem. 2018. 48: 2607-2655. 
  16. Nam SM, Kim YB. The effects of platelet-rich plasma on hypertrophic scars fibroblasts. Int Wound J. 2018. 15: 547-554.  https://doi.org/10.1111/iwj.12896
  17. Poulios E, Mykoniatis I, Pyrgidis N, Zilotis F, Kapoteli P, Kotsiris D, Kalyvianakis D, Hatzichristou D. Platelet-rich plasma (prp) improves erectile function: A double-blind, randomized, placebo-controlled clinical trial. J Sex Med. 2021. 18: 926-935.  https://doi.org/10.1016/j.jsxm.2021.03.008
  18. Sfakianoudis K, Simopoulou M, Nitsos N, Rapani A, Pappas A, Pantou A, Chronopoulou M, Deligeoroglou E, Koutsilieris M, Pantos K. Autologous platelet-rich plasma treatment enables pregnancy for a woman in premature menopause. J Clin Med. 2018. 8. 
  19. Singal PK, Khaper N, Palace V, Kumar D. The role of oxidative stress in the genesis of heart disease. Cardiovasc Res. 1998. 40: 426-432.  https://doi.org/10.1016/S0008-6363(98)00244-2
  20. Singh A, Kukreti R, Saso L, Kukreti S. Oxidative stress: A key modulator in neurodegenerative diseases. Molecules. 2019. 24.
  21. Steinhorn B, Sorrentino A, Badole S, Bogdanova Y, Belousov V, Michel T. Chemogenetic generation of hydrogen peroxide in the heart induces severe cardiac dysfunction. Nat Commun. 2018. 9: 4044. 
  22. Zhang Q, Liu J, Duan H, Li R, Peng W, Wu C. Activation of nrf2/ho-1 signaling: An important molecular mechanism of herbal medicine in the treatment of atherosclerosis via the protection of vascular endothelial cells from oxidative stress. J Adv Res. 2021. 34: 43-63.  https://doi.org/10.1016/j.jare.2021.06.023