DOI QR코드

DOI QR Code

Hematological characteristics and endocrine profiles of cloned dromedary camels (Camelus dromedaries)

  • 투고 : 2023.11.15
  • 심사 : 2024.01.19
  • 발행 : 2024.03.31

초록

Background: Somatic cell nuclear transfer (SCNT) is a prominent technology that can preserve superior genetic traits of animals and expand the population in a short time. Hematological characters and endocrine profiles are important elements that demonstrate the stability of the physiological state of cloned animals. To date, several studies regarding cloned camels with superior genes have been conducted. However, detailed hemato-physiological assessments to prove that cloned camels are physiologically normal are limited. In this study, We evaluated the hemato-physiological characteristics of cloned male and female dromedary camels (Camelus dromedaries). Methods: Therefore, we analyzed variations in hematological characteristics and endocrine profiles between cloned and non-cloned age-matched male and female dromedary camels (Camelus dromedaries). Two groups each of male and female cloned and non-cloned camels were monitored to investigate the differences in hemato-physiological characteristics. Results: All the animals were evaluated by performing complete blood count (CBC), serum chemistry, and endocrinological tests. We found no significant difference between the cloned and non-cloned camels. Furthermore, the blood chemistry and endocrine profiles in male and female camels before maturity were similar. Conclusions: These results suggest that cloned and non-cloned camels have similar hematological characteristics and endocrine parameters.

키워드

과제정보

This project was supported by the Patronage of H.H. Sheikh Mansour bin Zayed Al Nahyan, Deputy Prime Minister of the UAE and the Minister of Presidential Affairs. We acknowledge his support and inspiration in the initiation and mentoring of this project, without which this project would not have been possible.

참고문헌

  1. Campbell KH, McWhir J, Ritchie WA, Wilmut I. 1996. Sheep cloned by nuclear transfer from a cultured cell line. Nature 380:64-66. https://doi.org/10.1038/380064a0
  2. Cibelli JB, Campbell KH, Seidel GE, West MD, Lanza RP. 2002. The health profile of cloned animals. Nat. Biotechnol. 20:13-14. https://doi.org/10.1038/nbt0102-13
  3. Elitok B and Cirak AC. 2018. Clinical, hematological and blood biochemical features of camels. MOJ Immunol. 6:288-295.
  4. Fairburn HR, Young LE, Hendrich BD. 2002. Epigenetic reprogramming: how now, cloned cow? Curr. Biol. 12:R68-R70. https://doi.org/10.1016/S0960-9822(01)00677-7
  5. Galli C, Lagutina I, Perota A, Colleoni S, Duchi R, Lucchini F, Lazzari G. 2012. Somatic cell nuclear transfer and transgenesis in large animals: current and future insights. Reprod. Domest. Anim. 47 Suppl 3:2-11.
  6. Gu T, Shi J, Luo L, Li Z, Yang J, Cai G, Zheng E, Hong L, Wu Z. 2019. Study on hematological and biochemical characters of cloned Duroc pigs and their progeny. Animals (Basel) 9:912.
  7. Harper EJ, Hackett RM, Wilkinson J, Heaton PR. 2003. Age-related variations in hematologic and plasma biochemical test results in Beagles and Labrador Retrievers. J. Am. Vet. Med. Assoc. 223:1436-1442. https://doi.org/10.2460/javma.2003.223.1436
  8. Hashizume T, Ohtsuki K, Matsumoto N. 2000. Plasma insulinlike growth factor-I concentrations increase during the estrous phase in goats. Domest. Anim. Endocrinol. 18:253-263. https://doi.org/10.1016/S0739-7240(99)00082-X
  9. Hirose M, Hada M, Kamimura S, Matoba S, Honda A, Motomura K, Ogonuki N, Shawki HH, Inoue K, Takahashi S, Ogura A. 2018. Aberrant imprinting in mouse trophoblast stem cells established from somatic cell nuclear transfer-derived embryos. Epigenetics 13:693-703. https://doi.org/10.1080/15592294.2018.1507199
  10. Jin JX, Lee S, Taweechaipaisankul A, Kim GA, Lee BC. 2017. The HDAC inhibitor LAQ824 enhances epigenetic reprogramming and in vitro development of porcine SCNT embryos. Cell. Physiol. Biochem. 41:1255-1266. https://doi.org/10.1159/000464389
  11. Kashim MIAM, Hasim NA, Zin DMM, Amin L, Mokhtar MH, Shahimi S, Mutalib SA. 2021. Animal cloning and consumption of its by-products: a scientific and Islamic perspectives. Saudi J. Biol. Sci. 28:2995-3000. https://doi.org/10.1016/j.sjbs.2021.02.040
  12. Kim MJ, Oh HJ, Hwang SY, Hur TY, Lee BC. 2018. Health and temperaments of cloned working dogs. J. Vet. Sci. 19:585-591. https://doi.org/10.4142/jvs.2018.19.5.585
  13. Landry AM, Landry DJ, Gentry LR, Green HL, Reggio B, Koonce KL, Echelard Y, Godke RA. 2005. Endocrine profiles and growth patterns of cloned goats. Cloning Stem Cells 7:214-225. https://doi.org/10.1089/clo.2005.7.214
  14. Lanza RP, Cibelli JB, Faber D, Sweeney RW, Henderson B, Nevala W, West MD, Wettstein PJ. 2001. Cloned cattle can be healthy and normal. Science 294:1893-1894. https://doi.org/10.1126/science.1063440
  15. Lee BC, Kim MK, Jang G, Oh HJ, Yuda F, Kim HJ, Hossein MS, Kim JJ, Kang SK, Schatten G, Hwang WS. 2005. Dogs cloned from adult somatic cells. Nature 436:1102.
  16. Lee SL. 2014. Endocrine profiles and blood chemistry patterns of cloned miniature pigs in the post-puberty period. J. Emb. Trans. 29:119-125.
  17. Lin L, Li Q, Zhang L, Zhao D, Dai Y, Li N. 2008. Aberrant epigenetic changes and gene expression in cloned cattle dying around birth. BMC Dev. Biol. 8:14.
  18. Mundim AV, Coelho AO, Hortencio SM, Guimaraes EC, Espindola FS. 2007. Influence of age and sex on the serum biochemical profile of Doberman dogs in the growth phase. Comp. Clin. Path. 16:41-46. https://doi.org/10.1007/s00580-006-0653-z
  19. Olsson PO, Tinson AH, Al Shamsi N, Kuhad KS, Singh R, Son YB, Jeong Y, Jeong YW, Cai L, Sakaguchi K, Kim S, Choi EJ, Yu X, Hwang WS. 2021. Blastocyst formation, embryo transfer and breed comparison in the first reported large scale cloning of camels. Sci. Rep. 11:14288.
  20. Ouyang H, Han J, Huang Y. 2021. Pig cloning using somatic cell nuclear transfer. Methods Mol. Biol. 2239:1-18. https://doi.org/10.1007/978-1-0716-1084-8_1
  21. Park JE, Kim MK, Kang JT, Oh HJ, Hong SG, Kim DY, Jang G, Lee BC. 2010. Growth and hematologic characteristics of cloned dogs derived from adult somatic cell nuclear transfer. Cell. Reprogram. 12:141-150. https://doi.org/10.1089/cell.2009.0044
  22. Schmidt M, Winther KD, Secher JO, Callesen H. 2015. Postmortem findings in cloned and transgenic piglets dead before weaning. Theriogenology 84:1014-1023. https://doi.org/10.1016/j.theriogenology.2015.05.037
  23. Selokar NL, Sharma P, Saini M, Sheoran S, Rajendran R, Kumar D, Sharma RK, Motiani RK, Kumar P, Jerome A, Khanna S, Yadav PS. 2019. Successful cloning of a superior buffalo bull. Sci. Rep. 9:11366.
  24. Silveira MM, Salgado Bayao HX, Dos Santos Mendonca A, Borges NA, Vargas LN, Caetano AR, Rumpf R, Franco MM. 2018. DNA methylation profile at a satellite region is associated with aberrant placentation in cloned calves. Placenta 70:25-33. https://doi.org/10.1016/j.placenta.2018.08.007
  25. Singh B, Mal G, Gautam SK, Mukesh M. 2019. Reproduction biotechnology in camelids. In: Sanjeev K. Gautam (Ed(s).), Advances in Animal Biotechnology, Springer, Cham, pp. 145-153.
  26. Son YB, Jeong YI, Jeong YW, Yu X, Olsson PO, Cai L, Choi EJ, Hossein MS, Tinson A, Singh KK, Rajesh S, Noura AS, Hwang WS. 2021. Comparison of pregnancy outcomes following the transfer of early-developmental stage embryos and blastocysts produced by somatic cell nuclear transfer in Camelus dromedarius. Anim. Reprod. Sci. 233:106842.
  27. Swanson KS, Kuzmuk KN, Schook LB, Fahey GC Jr. 2004. Diet affects nutrient digestibility, hematology, and serum chemistry of senior and weanling dogs. J. Anim. Sci. 82:1713-1724. https://doi.org/10.2527/2004.8261713x
  28. Wang M, Sun Z, Yu T, Ding F, Li L, Wang X, Fu M, Wang H, Huang J, Li N, Dai Y. 2017. Large-scale production of recombinant human lactoferrin from high-expression, marker-free transgenic cloned cows. Sci. Rep. 7:10733.
  29. Wani NA, Wernery U, Hassan FA, Wernery R, Skidmore JA. 2010. Production of the first cloned camel by somatic cell nuclear transfer. Biol. Reprod. 82:373-379. https://doi.org/10.1095/biolreprod.109.081083
  30. Whitelaw CB, Sheets TP, Lillico SG, Telugu BP. 2016. Engineering large animal models of human disease. J. Pathol. 238:247-256. https://doi.org/10.1002/path.4648