The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Paeonia anomala L. water extract attenuates differentiation and fat accumulation in 3T3-L1 preadipocytes

Paeonia anomala L.의 물 추출물이 3T3-L1 지방 전구세포의 분화 및 지방 축적에 미치는 영향

  • Moon-Yeol Choi (Department of Herbal Pharmacology, College of Korean Medicine, Daegu Haany University) ;
  • Mi Hyung Kim (Department of Herbal Pharmacology, College of Korean Medicine, Daegu Haany University) ;
  • Mi Ryeo Kim (Department of Herbal Pharmacology, College of Korean Medicine, Daegu Haany University)
  • 최문열 (대구한의대 한의과대학 본초약리학교실) ;
  • 김미형 (대구한의대 한의과대학 본초약리학교실) ;
  • 김미려 (대구한의대 한의과대학 본초약리학교실)
  • Received : 2024.01.27
  • Accepted : 2024.03.25
  • Published : 2024.03.30
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Abstract

Objective : Obesity is a disease caused by energy imbalance and increases the risk of complications such as cardiovascular disease and cancer. Additionally, a recent study reported that excessive production of ROS stimulates the expression of transcription factors related to adipogenesis, such as PPAR-𝛾, C/EBP-𝛼 and 𝛽, in 3T3-L1 cells. In this study, Paeonia anomala L. (PAL) was selected as a candidate for improving and preventing obesity from Mongolian medical literature, and its effect on fat metabolism was observed in vitro. Methods : The activity of PAL extract against lipid accumulation was measured in 3T3-L1 cells through Oil red O staining. Additionally, the expression of lipid metabolism-related proteins was measured by western blot. Results : Intracellular lipid accumulation was significantly lower in the treatment group than in the control group. In addition, pAMPK/AMPK protein expression in the treatment group tended to increase in a concentration-dependent manner, and PPAR-𝛾 protein expression in the treatment group showed a significant decrease in a concentration-dependent manner compared to the control group. Conclusion : These results suggest that PAL extract may have an anti-obesity effect, and it is believed that it can be used to develop functional materials for the treatment of obesity.

Keywords

Acknowledgement

이 논문은 농촌진흥청 (PJ015272032021)과 한국연구재단(2021R1A2C201471711)의 지원을 받아 수행된 연구입니다.

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