Korean Journal of Clinical Laboratory Science (대한임상검사과학회지)

Korean Society for Clinical Laboratory Science (대한임상검사과학회)
권호 표지
DOI QR코드

DOI QR Code

Distribution of Aerobic Intestinal Microorganisms in the Feces of the Striped Field Mouse (Apodemus agrarius coreae) in Jeju

제주지역 야생 등줄쥐(Apodemus agrarius coreae) 분변의 호기성 장내 미생물 분포

  • Jiro KIM (Department of Clinical Laboratory Science, Cheju Halla University) ;
  • Yun-Hee OH (Department of Nursing, Cheju Halla University) ;
  • Moo-Sang CHONG (Department of Clinical Laboratory Science, Cheju Halla University)
  • 김지로 (제주한라대학교 임상병리과) ;
  • 오윤희 (제주한라대학교 간호학과) ;
  • 정무상 (제주한라대학교 임상병리과)
  • Received : 2024.01.27
  • Accepted : 2024.02.15
  • Published : 2024.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

This study examined the fecal samples of striped field mice (Apodemus agrarius coreae) captured in Jeju Special Self-Governing Province. Fecal samples, including the colon and other intestinal organs, were collected and subjected to aerobic culture to investigate the distribution of intestinal microorganisms. Gram staining of the aerobic cultured bacterial colonies from 36 fecal samples revealed the predominant presence of gram-negative bacilli in all samples. Among the 36 samples, gram-negative bacilli were identified in 36 strains (100%), gram-positive cocci in 21 strains (58.3%), and gram-positive bacilli in 15 strains (41.7%), while no gram-negative cocci were observed. The gram-negative bacilli cultured from the 36 samples were identified using the Vitek 2 system, and all were determined to be Escherichia coli (E. coli) strains. In addition, one sample was concurrently identified with E. coli and Enterobacter cloacae strains. The antimicrobial susceptibility testing for the identified E. coli strains did not include all antibiotics, but one strain exhibited intermediate resistance to cefoxitin. No pathogenic bacteria were present in the fecal samples of the scrub typhus-infected rodents, which are vectors for chigger-borne diseases affecting humans and animals.

제주특별자치도에 서식하는 등줄쥐를 포획하여 대장을 포함한 내부장기에서 분변 검체를 채취한 후, 장내 미생물을 호기적 배양하여 등줄쥐 분변에서 배양된 장내 미생물 분포를 조사하였다. 등줄쥐 분변 36개의 검체를 호기성 배양된 세균 집락을 그람염색 한 결과 모든 검체에서 그람음성막대균이 우세하게 관찰되었다. 36 검체 중 그람음성막대균은 36 균주(100%), 그람양성알균은 21 균주(58.3%), 그람양성막대균은 15 균주(41.7%)로 나타났으며 그람음성알균은 관찰되지 않았다. 36개의 검체에서 배양된 그람음성막대균은 Vitek 2 system을 이용하여 동정한 결과 모두 Escherichia coli (E. coli) 균종으로 동정되었으며, 1개 검체는 E. coli 균종과 Enterobacter cloacae 균종이 동시에 동정되었다. 분리된 E. coli 36 균주 중 항균제에 내성을 보이는 균주는 발견되지 않았으며, 1 균주만 cefoxitin 항균제에 중등도 내성을 보이는 것으로 나타났다. 진드기, 설치류 매개 감염병의 원인이 되는 등줄쥐의 분변에서는 병원성 세균은 존재하지 않았다.

Keywords

References

  1. Korea Disease Control and Prevention Agency (KDCA). Guidelines for the management of tick and rodent-borne infectious diseases in 2023. KDCA: 2023.
  2. Kim SH, Jang JY. Correlations between climate change-related infectious diseases and meteorological factors in Korea. J Prev Med Public Health. 2010;43:436-444. https://doi.org/10.3961/jpmph.2010.43.5.436
  3. Rossati A. Global warming and its health impact. Int J Occup Environ Med. 2017;8:7-20. https://doi.org/10.15171/ijoem.2017.963
  4. Lee HW. Hemorrhagic fever with renal syndrome in Korea. Rev Infect Dis. 1989;11 Suppl 4:S864-S8676.
  5. Kim YK, Lee SC, Kim C, Heo ST, Choi C, Kim JM. Clinical and laboratory predictors of oliguric renal failure in haemorrhagic fever with renal syndrome caused by Hantaan virus. J Infect. 2007;54:381-386. https://doi.org/10.1016/j.jinf.2006.07.006
  6. Kim YS, Ahn C, Han JS, Kim S, Lee JS, Lee PW. Hemorrhagic fever with renal syndrome caused by the Seoul virus. Nephron. 1995; 71:419-427. https://doi.org/10.1159/000188762
  7. Lee HJ, Park C. Distribution variations of chigger mites collected in Jinan, Jeollabuk-do. Korean J Clin Lab Sci. 2021;53:317-325. https://doi.org/10.15324/kjcls.2021.53.4.317
  8. Yoon MH, Phillips CJ, Kim I, Oh HS. Biogeography, genetic structure, and speciation in the striped field mouse, Apodemus agrarius, in Southern Korea. Genes Genom. 2004;26:15-28.
  9. Meerburg BG, Singleton GR, Kijlstra A. Rodent-borne diseases and their risks for public health. Crit Rev Microbiol. 2009; 35:221-270. https://doi.org/10.1080/10408410902989837
  10. Al-Eitan L, Alnemri M, Alkhawaldeh M, Mihyar A. Rodent-borne viruses in the region of Middle East. Rev Med Virol. 2023;33:e2440. https://doi.org/10.1002/rmv.2440
  11. Doran JW, Schepers JS, Swanson NP. Chemical and bacteriological quality of pasture runoff. J Soil Water Conserv. 1981;36:166-171.
  12. Murray KS, Fisher LE, Therrien J, George B, Gillespie J. Assessment and use of indicator bacteria to determine sources of pollution to an urban river. J Great Lakes Res. 2001;27:220-229. https://doi.org/10.1016/S0380-1330(01)70635-1
  13. Kim JG, Lee JH, Kwon HK. The distribution of indicator microorganisms and identification of antibiotic resistant strains in domestic animal feces. J Environ Health Sci. 2011;37:289-297. https://doi.org/10.5668/JEHS.2011.37.4.289
  14. Chong MS. Classification of intestinal microorganisms cultured from the feces of pet dogs fed commercial feed. J Korean Health Fundam Med Sci. 2020;13:122-126.
  15. Song H. Distribution and population density of rodents and chigger mites in Gokseong-gun of Jeollanam-do, Korea. Korean J Clin Lab Sci. 2016;48:242-246. https://doi.org/10.15324/kjcls.2016.48.3.242
  16. Traub R, Wisseman CL Jr. The ecology of chigger-borne rickettsiosis (scrub typhus). J Med Entomol. 1974;11:237-303. https://doi.org/10.1093/jmedent/11.3.237
  17. Jackson EB, Danauskas JX, Smadel JE, Fuller HS, Coale MC, Bozeman FM. Occurrence of Rickettsia tsutsugamushi in Korean rodents and chiggers. Am J Hyg. 1957;66:309-320. https://doi.org/10.1093/oxfordjournals.aje.a119904
  18. Yeon SC, Kim JT, Na KJ, Yun YM, Lee KK, Ko YJ, et al. Establishment of sample collection system to investigate efficiently wildlife disease. Research report. Incheon: National Institute of Environmental Research; 2016 Dec. NIER-SP2016-38.
  19. Yoo SM. Development of standard operating procedure manuals for microbial pathogen resource banks. Research report. Cheongju: Korea Centers for Disease Control and Prevention; 2008 Sep. 2008-E00183-00.
  20. Oh J, Whang K, Jung H, Park J. Comparison of the pattern in semi-quantitative sputum cultures based on different endotracheal suction techniques. Korean J Crit Care Med. 2012;27:70-74. https://doi.org/10.4266/kjccm.2012.27.2.70
  21. Infectious Diseases Homepage. The results of the National Infectious Disease Surveillance 2023. Korea Disease Control and Prevention Agency (KDCA) [Internet]. KDCA [cited 2024 February 15]. Available from: https://dportal.kdca.go.kr/pot/is/summary.do
  22. Lee HJ, Park C. Density of chigger mites as tsutsugamushi vectors collected from Jinan, Jeollabuk-do. Korean J Clin Lab Sci. 2020;52:364-371. https://doi.org/10.15324/kjcls.2020.52.4.364
  23. Chung KA, Song HJ, Lee HJ, Park C, Seo MY. Distribution of tick vectors of severe fever with thrombocytopenia syndrome virus (SFTSV) collected from four environments in Jeju. Korean J Clin Lab Sci. 2020;52:356-363. https://doi.org/10.15324/kjcls.2020.52.4.356
  24. Seo CW. Analysis of factors related to regional occurrence distribution of scrub typhus: 2012-2016. Korean J Clin Lab Sci. 2019;51:420-427. https://doi.org/10.15324/kjcls.2019.51.4.420
  25. Kim DY, Kim DM. The most common mite- and tick-borne infectious diseases in Korea: scrub typhus and severe fever thrombocytopenia syndrome. Korean J Med. 2018;93:416-423. https://doi.org/10.3904/kjm.2018.93.5.416
  26. Lee DK. Ecological characteristics and current status of infectious disease vectors in South Korea. J Korean Med Assoc. 2017;60:458-467. https://doi.org/10.5124/jkma.2017.60.6.458