한국응용곤충학회지 (Korean journal of applied entomology)

  • 제63권1호
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  • Pages.33-42
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  • 2024
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  • 1225-0171(pISSN)
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  • 2287-545X(eISSN)
한국응용곤충학회 (Korean Society of Applied Entomology)
권호 표지
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복숭아혹진딧물 야외개체군의 λ-cyhalothrin, imidacloprid, 그리고 flupyradifurone에 대한 저항성 모니터링과 점 돌연변이 분석

Resistance Monitoring and Analysis of Point Mutations to λ-cyhalothrin, Imidacloprid, and Flupyradifurone in Field-collected Populations of Myzus persicae (Hemiptera: Aphididae)

  • 문하현 (충북대학교 식물의학과) ;
  • 이유노 (충북대학교 식물의학과) ;
  • 강동현 (충북대학교 식물의학과) ;
  • 김세은 (충북대학교 식물의학과) ;
  • 김현경 (충북대학교 식물의학과) ;
  • 구현나 (충북대학교 식물의학과) ;
  • 김길하 (충북대학교 식물의학과)
  • Ha Hyeon Moon (Department of Plant Medicine, Chungbuk National University) ;
  • Yuno Lee (Department of Plant Medicine, Chungbuk National University) ;
  • Dong-Hyun Kang (Department of Plant Medicine, Chungbuk National University) ;
  • Se Eun Kim (Department of Plant Medicine, Chungbuk National University) ;
  • Hyun Kyung Kim (Department of Plant Medicine, Chungbuk National University) ;
  • Hyun-Na Koo (Department of Plant Medicine, Chungbuk National University) ;
  • Gil-Hah Kim (Department of Plant Medicine, Chungbuk National University)
  • 투고 : 2024.01.09
  • 심사 : 2024.02.15
  • 발행 : 2024.03.01
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초록

복숭아혹진딧물(Myzus persicae)은 다식성으로 담배, 감자, 고추, 배추, 복숭아 등에 심각한 피해를 입히는 대표적인 농업해충이다. 본 연구에서는 국내 복숭아혹진딧물 야외개체군의 λ-cyhalothrin, imidacloprid 및 flupyradifurone에 대한 약제 저항성 발달 수준과 점 돌연변이(R81T, L1014F, M918L)의 발생 여부를 확인하였다. 또한, qRT-PCR을 통해 사이토크롬 P450 유전자인 CYP6CY3 발현량을 확인하였다. 그 결과, λ-cyhalothrin은 저항성비(Resistance Ratio, RR)가 12개 모든 지역이 > 200으로 높은 저항성을 보였다. Imidacloprid와 flupyradifurone은 YS, UR, HY, 그리고 WJ 개체군에서 > 200의 저항성비로 높은 저항성을 나타냈다. R81T는 12개 집단 중 약 50%, L1014F는 약 33.3%, M918L은 100%에서 발현하였다. 또한 qRT-PCR을 통해 imidacloprid 저항성 개체에서 subunit CYP6CY3의 발현량이 높게 나타난 것을 확인하였다. 이러한 결과는 M918L 점 돌연변이는 λ-cyhalothrin 저항성 진단마커로, R81T와 CYP6CY3의 높은 발현은 imidacloprid 저항성 진단마커로 활용이 가능하다는 것을 시사한다.

The green peach aphid, Myzus persicae is a representative agricultural insect pest that is polyphagous and causes serious damage to tobacco, potatoes, peppers, cabbage, and peaches. In this study, we analyzed the level of development of insecticide resistance to λ-cyhalothrin, imidacloprid, and flupyradifurone and the point mutations (R81T, L1014F, M918L) in 12 field populations of M. persicae. In addition, the expression level of CYP6CY3, a cytochrome P450 gene, was analyzed through qRT-PCR. As a result, λ-cyhalothrin showed high resistance ratio (RR) of > 200 in all 12 populations. Imidacloprid and flupyradifurone showed high RR of >200 in YS, UR, HY, and WJ populations. The R81T was detected in approximately 50%, L1014F in approximately 33.3%, and M918L in 100% of the 12 populations. Additionally, the expression level of subunit CYP6CY3 was highest in imidacloprid-resistant population (YS). These results suggest that M918L point mutation can be used as λ-cyhalothrin-resistance molecular diagnostic and R81T point mutation and the high expression of CYP6CY3 can be used as imidacloprid-resistance molecular diagnostic markers.

키워드

과제정보

이 성과는 농촌진흥청 지원을 받아 수행된 연구임(RS-2022-RD-010420).

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