Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Network pharmacology analysis of Jakyakgamchotang with corydalis tuber for anti-inflammation

작약감초탕 가 현호색의 항염증 기전에 대한 네트워크 약리학적 분석

  • Young-Sik Kim (Department of Herbology, College of Korean Medicine, Woosuk University) ;
  • Hongjun Kim (Department of Fomula Science, College of Korean Medicine, Woosuk University) ;
  • Han-bin Park (Department of Fomula Science, College of Korean Medicine, Woosuk University) ;
  • Seungho Lee (Department of Pathology, College of Korean Medicine, Woosuk University)
  • 김영식 (우석대학교 한의과대학 본초학교실) ;
  • 김홍준 (우석대학교 한의과대학 방제학교실) ;
  • 박한빈 (우석대학교 한의과대학 방제학교실) ;
  • 이승호 (우석대학교 한의과대학 병리학교실)
  • Received : 2023.12.07
  • Accepted : 2024.02.06
  • Published : 2024.02.28
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Abstract

Objectives : The purpose of this study was to investigate the molecular targets and pathways of anti-inflammatory effects of Jakyakgamchotang with corydalis tuber (JC) using network pharmacology. Methods : The compounds in constituent herbal medicines of JC were searched in TCM systems pharmacology (TCMSP). Target gene informations of the components were collected using chemical-target interactions database provided by Pubchem. Afterwards, network analysis between compounds and inflammation-related target genes was performed using cytoscape. Go enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were performed on inflammation-related targets using DAVID database. Results : 70 active compounds related to inflammation were identified, and 295 target genes related to the anti-inflammatory activity of the compound of JC were identified. In the Go biological process DB and KEGG pathway DB, "inflammatory response", "cellular response to lipopolysaccharide", "positive regulation of interleukin-6 production", and "positive regulation of protein kinase B. signaling", "positive regulation of ERK1 and ERK2 cascade", "positive regulation of I-kappaB kinase/NF-kappaB signaling", "negative regulation of apoptotic process", and "PI3K-Akt signaling pathway" were found to be mechanisms related to the anti-inflammatory effects related to the target genes of JC. The main compounds predicted to be involved in the anti-inflammatory effect of JC were quercetin, licochalcone B, (+)-catechin, kaempferol, and emodin. Conclusions : This study provides the molecular targets and potential pathways of JC on inflammation. It can be used as a basic data for using JC for various inflammatory disease in traditional korean medicine clinic.

Keywords

Acknowledgement

이 논문은 우석대학교 교내학술연구비 지원에 의하여 연구됨

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