The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Effect of Astragalus membranaceus-postbiotics Polysaccharide Changed by Lactic Acid Bacteria on Macrophage

유산균에 의해 변화된 황기-포스트바이오틱스 다당류가 대식세포에 미치는 영향

  • Yeon Suk Kim (Major in Food and Nutrition, Korea National University of Transportation) ;
  • Hyun Young Shin (Transdisciplinary Major in Learning Health System, Dept. of Integrated Biomedical & Life Science, Korea University) ;
  • Won Bi Jeong (Major in Food and Nutrition, Korea National University of Transportation) ;
  • Eun Ji Ha (Major in Food and Nutrition, Korea National University of Transportation) ;
  • Ja Pyeong Koo (Major in Food and Nutrition, Korea National University of Transportation) ;
  • Ji-Young Shin (Cosis-Bio Co., Ltd.) ;
  • Kwang-Won Yu (Major in Food and Nutrition, Korea National University of Transportation)
  • 김연숙 (한국교통대학교 식품영양학전공) ;
  • 신현영 (고려대학교 대학원 의생명융합과학과 러닝헬스시스템 융합전공) ;
  • 정원비 (한국교통대학교 식품영양학전공) ;
  • 하은지 (한국교통대학교 식품영양학전공) ;
  • 구자평 (한국교통대학교 식품영양학전공) ;
  • 신지영 ((주)코시스바이오) ;
  • 유광원 (한국교통대학교 식품영양학전공)
  • Received : 2023.12.13
  • Accepted : 2024.01.23
  • Published : 2024.02.28
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Abstract

To increase industrial applicability of Astragalus membranaceus (AM) as immunostimulating materials, hot-water extract (AME) was prepared from AM and fermented with Kimchi-lactic acid bacteria (Lactobacillus sakei & Leuconostoc mesenteroides) to prepare fermented AM-postbiotics (FAME). Although FAME prepared from AM-postbiotics did not show a significant enhancement in macrophage stimulating activity compared to non-fermented AME, crude polysaccharide (FAME-CP) fractionated by EtOH precipitation from FAME showed significantly higher macrophage stimulating activity than AME-CP. Compared to AME-CP, FAME-CP showed dramatic changes in component sugar and molecular weight distribution. FAME-CP was a polysaccharide with a major molecular weight distribution of 113.4 kDa containing Man (44.2%), Glc (19.3%), Gal (10.2%), GalA (10.2%), and Ara (7.4%) as sugar components. FAME-CP with enhanced macrophage stimulatory activity not only increased expression levels of mRNA genes encoding macrophage-activated factors (iNOS, TNF-α, MCP-1, IL-6, and COX-2), but also led the nuclear translocation of activated p65 and c-Jun. In conclusion, crude polysaccharide from AM-postbiotics fermented with lactic acid bacteria could increase industrial applicability as a functional material with enhanced immunostimulating activity than AME-CP.

Keywords

Acknowledgement

본 논문은 2023년도 교육부의 재원으로 한국연구재단의 지원을 받아 수행된 지자체-대학 협력기반 지역혁신 사업의 결과입니다(2021RIS-001; 프로젝트랩).

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