DOI QR코드

DOI QR Code

Dextran Sodium Sulfate 유발 마우스 대장염에서 Lactiplantibacillus plantarum JSA22 쌀 발효물의 효과

Effect of Lactiplantibacillus plantarum JSA22-fermented Rice Drinks against Dextran Sodium Sulfate-induced Colitis in Mice

  • 유지송 (원광대학교 의과대학 생리학교실 ) ;
  • 선다현 (원광대학교 의과대학 생리학교실 ) ;
  • 최현석 (원광대학교 의과대학 생리학교실 ) ;
  • 김민섭 (원광대학교 의과대학 생리학교실 ) ;
  • 유명환 (원광대학교 의과대학 생리학교실 ) ;
  • 진종식 (전북대학교 한약자원학과) ;
  • 김동근 (전북대학교 한약자원학과 ) ;
  • 최혜선 (농촌진흥청 ) ;
  • 박정호 (강북삼성병원 소화기내과) ;
  • 김용성 (주식회사 것앤드푸드헬스케어 ) ;
  • 이문영 (원광대학교 의과대학 생리학교실)
  • Jisong You (Dept. of Physiology, Digestive Disease Research Institute, Wonkwang University School of Medicine) ;
  • Dahyun Seon (Dept. of Physiology, Digestive Disease Research Institute, Wonkwang University School of Medicine) ;
  • Hyun Seok Choi (Dept. of Physiology, Digestive Disease Research Institute, Wonkwang University School of Medicine) ;
  • Min Seob Kim (Dept. of Physiology, Digestive Disease Research Institute, Wonkwang University School of Medicine) ;
  • Myeong Hwan Yu (Dept. of Physiology, Digestive Disease Research Institute, Wonkwang University School of Medicine) ;
  • Jong Sik Jin (Dept. of Oriental Medicine Resources, Jeonbuk National University) ;
  • Dong Keun Kim (Dept. of Oriental Medicine Resources, Jeonbuk National University) ;
  • Hye Sun Choi (National Institute of Crop Science, Rural Development Administration) ;
  • Jung Ho Park (Dept. of Gastroenterology, Kangbuk Samsung Hospital) ;
  • Yong Sung Kim (Gut & Food Healthcare Co., Ltd.) ;
  • Moon Young Lee (Dept. of Physiology, Digestive Disease Research Institute, Wonkwang University School of Medicine)
  • 투고 : 2023.11.15
  • 심사 : 2024.01.15
  • 발행 : 2024.02.28

초록

This study investigated the effect of Lactiplantibacillus plantarum JSA22-fermented rice drinks on dextran sodium sulfate (DSS)-induced colitis in mice. Twenty-four mice were randomly assigned; No colitis (Con), colitis with tap water (DSS-only), colitis with unfermented rice (DSS-UFR), and colitis with fermented rice (DSS-FR). After inducing colitis with 2% DSS for 5 days, they were given Tap water, UFR drink, or FR drink for an additional 6 days. The DSS-FR group had significantly lower Disease Activity Index (DAI) scores compared to the DSS-only group, but no significant difference with the DSS-UFR group. Colon length was reduced in the DSS-only group. The DSS-only group had significantly higher IL-6 mRNA levels compared to the Con group, while the DSS-FR groups showed significantly lower IL-6 mRNA levels compared to the DSS-only group. These results suggest that rice drinks fermented with Lactiplantibacillus Plantarum JSA22 ameliorate the severity of DSS-colitis, by potentially reducing proinflammatory cytokines.

키워드

과제정보

본 논문은 농촌진흥청 연구개발사업(PJ016707)으로 수행된 결과입니다.

참고문헌

  1. Ahn YJ, Choi HS. 2014. Potential probiotic properties of exopolysaccharide producing lactic acid bacteria isolated from fermented soybean product. J Korean Soc Food Sci Nutr 43:749-755 https://doi.org/10.3746/jkfn.2014.43.5.749
  2. Alex P, Zachos NC, Nguyen T, Gonzales L, Chen TE, Conklin LS, Centola M, Li X. 2009. Distinct cytokine patterns identified from multiplex profiles of murine DSS and TNBS-induced colitis. Inflamm Bowel Dis 15:341-352 https://doi.org/10.1002/ibd.20753
  3. Belizario JE, Faintuch J, Garay-Malpartida M. 2018. Gut microbiome dysbiosis and immunometabolism: New frontiers for treatment of metabolic diseases. Mediators Inflammation 2018:2037838
  4. Chassaing B, Aitken JD, Malleshappa M, Vijay-Kumar M. 2014. Dextran sulfate sodium (DSS)-induced colitis in mice. Curr Protoc Immunol 104:15.25.1-15.25.14 https://doi.org/10.1002/0471142735.im1525s104
  5. Choi HS, Seong H, Kim SA, Song Y, Sim EY, Kang H, Han NS. 2023. Lysine-fortified rice germ yogurt fermented with Lactiplantibacillus plantarum JSA 22 and its beneficial health effects. J Funct Foods 109:105787
  6. Couto MR, Goncalves P, Magro F, Martel F. 2020. Microbiota-derived butyrate regulates intestinal inflammation: Focus on inflammatory bowel disease. Pharmacol Res 159:104947
  7. Eichele DD, Kharbanda KK. 2017. Dextran sodium sulfate colitis murine model: An indispensable tool for advancing our understanding of inflammatory bowel diseases pathogenesis. World J Gastroenterol 23:6016-6029 https://doi.org/10.3748/wjg.v23.i33.6016
  8. Hill C, Guarner F, Reid G, Gibson GR, Merenstein DJ, Pot B, Morelli L, Canani RB, Flint HJ, Salminen S, Calder PC, Sanders ME. 2014. Expert consensus document: The International Scientific Association for Probiotics and Prebiotics consensus statement on the scope and appropriate use of the term probiotic. Nat Rev Gastroenterol Hepatol 11:506-514 https://doi.org/10.1038/nrgastro.2014.66
  9. Kim KY, Son JD, Hwang SJ, Lee JK, Park JY, Park KI, Oh TW. 2023. Fermented glutinous rice extract mitigates DSS-induced ulcerative colitis by alleviating intestinal barrier function and improving gut microbiota and inflammation. Antioxidants 12:336
  10. Lee MG, Chun BH, Dhungana SK, Park JJ, Kim ID. 2020. Quality characteristics and antioxidant potential of rice-germ rice processed with different heat treatments. Int J Sci 9:16-21 https://doi.org/10.18483/ijSci.2280
  11. Lee MY, Choi SC, Kim YS. 2019. The role of gut microbiota and use of probiotics in the treatment of upper gastrointestinal diseases. Korean J Helicobacter Up Gastrointest Res 19:99-105 https://doi.org/10.7704/kjhugr.2019.19.2.99
  12. Liu YW, Su YW, Ong WK, Cheng TH, Tsai YC. 2011. Oral administration of Lactobacillus plantarum K68 ameliorates DSS-induced ulcerative colitis in BALB/c mice via the anti-inflammatory and immunomodulatory activities. Int Immunopharmacol 11:2159-2166 https://doi.org/10.1016/j.intimp.2011.09.013
  13. Lynch SV, Pedersen O. 2016. The human intestinal microbiome in health and disease. N Engl J Med 375:2369-2379 https://doi.org/10.1056/NEJMra1600266
  14. Nagaoka S. 2019. Yogurt production. In Kanauchi M (Ed.), Lactic Acid Bacteria: Methods and Protocols. pp.45-54. Humana
  15. Oh WS, Jung JC, Choi YM, Mun JY, Ku SK, Song CH. 2020. Protective effects of fermented rice extract on ulcerative colitis induced by dextran sodium sulfate in mice. Food Sci Nutr 8:1718-1728 https://doi.org/10.1002/fsn3.1460
  16. Ortega-Cava CF, Ishihara S, Rumi MAK, Kawashima K, Ishimura N, Kazumori H, Udagawa J, Kadowaki Y, Kinoshita Y. 2003. Strategic compartmentalization of toll-like receptor 4 in the mouse gut. J Immunol 170:3977-3985 https://doi.org/10.4049/jimmunol.170.8.3977
  17. Pan Y, Ning Y, Hu J, Wang Z, Chen X, Zhao X. 2021. The preventive effect of Lactobacillus plantarumZS62 on DSS-induced IBD by regulating oxidative stress and the immune response. Oxid Med Cell Longev 2021:9416794
  18. Park AM, Tsunoda I. 2018. Forensic luminol reaction for detecting fecal occult blood in experimental mice. Biotechniques 65:227-230 https://doi.org/10.2144/btn-2018-0017
  19. Saez-Lara MJ, Gomez-Llorente C, Plaza-Diaz J, Gil A. 2015. The role of probiotic lactic acid bacteria and bifidobacteria in the prevention and treatment of inflammatory bowel disease and other related diseases: A systematic review of randomized human clinical trials. BioMed Res Int 2015:505878
  20. Scheppach W, Sommer H, Kirchner T, Paganelli GM, Bartram P, Christl S, Richter F, Dusel G, Kasper H. 1992. Effect of butyrate enemas on the colonic mucosa in distal ulcerative colitis. Gastroenterology 103:51-56 https://doi.org/10.1016/0016-5085(92)91094-K
  21. Sultan N, Varney JE, Halmos EP, Biesiekierski JR, Yao CK, Muir JG, Gibson PR, Tuck CJ. 2022. How to implement the 3-phase FODMAP diet into gastroenterological practice. J Neurogastroenterol Motil 28:343-356 https://doi.org/10.5056/jnm22035
  22. Toumi R, Soufli I, Rafa H, Belkhelfa M, Biad A, Touil-Boukoffa C. 2014. Probiotic bacteria Lactobacillus and Bifidobacterium attenuate inflammation in dextran sulfate sodium-induced experimental colitis in mice. Int J Immunopathol Pharmacol 27:615-627 https://doi.org/10.1177/039463201402700418
  23. Wang G, Liu Y, Lu Z, Yang Y, Xia Y, Lai PFH, Ai L. 2019. The ameliorative effect of a Lactobacillus strain with good adhesion ability against dextran sulfate sodium-induced murine colitis. Food Funct 10:397-409 https://doi.org/10.1039/C8FO01453A
  24. Xia Y, Chen Y, Wang G, Yang Y, Song X, Xiong Z, Zhang H, Lai P, Wang S, Ai L. 2020. Lactobacillus plantarum AR113 alleviates DSS-induced colitis by regulating the TLR4/MyD88/NF-κB pathway and gut microbiota composition. J Funct Foods 67:103854
  25. Zhang Q, Wu Y, Wang J, Wu G, Long W, Xue Z, Wang L, Zhang X, Pang X, Zhao Y, Zhao L, Zhang C. 2016. Accelerated dysbiosis of gut microbiota during aggravation of DSS-induced colitis by a butyrate-producing bacterium. Sci Rep 6:27572