Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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The Anti-Diabetic Pinitol Improves Damaged Fibroblasts

  • Ji-Yong Jung (Amorepacific Corporation R&D Center) ;
  • Joong Hyun Shim (Department of Biohealth-Convergence, Seoul Women's University) ;
  • Su Hae Cho (Research Institute for Biomedical and Health Science, Konkuk University) ;
  • Il-Hong Bae (Amorepacific Corporation R&D Center) ;
  • Seung Ha Yang (Amorepacific Corporation R&D Center) ;
  • Jinsick Kim (Research Institute for Biomedical and Health Science, Konkuk University) ;
  • Hye Won Lim (Research Institute for Biomedical and Health Science, Konkuk University) ;
  • Dong Wook Shin (Research Institute for Biomedical and Health Science, Konkuk University)
  • Received : 2023.12.15
  • Accepted : 2023.12.22
  • Published : 2024.03.01
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Abstract

Pinitol (3-O-Methyl-D-chiro-inositol) has been reported to possess insulin-like effects and is known as one of the anti-diabetic agents to improve muscle, liver, and endothelial cells. However, the beneficial effects of pinitol on the skin are not well known. Here, we investigated whether pinitol had effects on human dermal fibroblasts (HDFs), and human dermal equivalents (HDEs) irradiated with ultraviolet A (UVA), which causes various damages including photodamage in the skin. We observed that pinitol enhanced wound healing in UVA-damaged HDFs. We also found that pinitol significantly antagonized the UVA-induced up-regulation of matrix metalloproteinase 1 (MMP1), and the UVA-induced down-regulation of collagen type I and tissue inhibitor of metalloproteinases 1 (TIMP1) in HDEs. Electron microscopy analysis also revealed that pinitol remarkably increased the number of collagen fibrils with regular banding patterns in the dermis of UVA-irradiated human skin equivalents. Pinitol significantly reversed the UVA-induced phosphorylation levels of ERK and JNK but not p38, suggesting that this regulation may be the mechanism underlying the pinitol-mediated effects on UVA-irradiated HDEs. We also observed that pinitol specifically increased Smad3 phosphorylation, which is representative of the TGF-β signaling pathway for collagen synthesis. These data suggest that pinitol exerts several beneficial effects on UVA-induced damaged skin and can be used as a therapeutic agent to improve skin-related diseases.

Keywords

Acknowledgement

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (2022R1A2C1093305).

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