Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Antioxidant effect of Cistanches Herba against hydrogen peroxide-induced oxidative stress in Leydig cells

육총용(肉蓯蓉)이 hydrogen peroxide에 의해 유도된 Leydig cell에 미치는 항산화 효과 연구

  • Mun Seog Chang (Department of Prescriptionology, College of Korean Medicine, Kyung Hee University) ;
  • Kwan Suk Bang (Department of Korean Medicine, College of Korean Medicine, Graduate School, Kyung Hee University) ;
  • Jeong Jin Lee (Department of Korean Medicine, College of Korean Medicine, Graduate School, Kyung Hee University) ;
  • Jun Young Hur (Department of Korean Medicine, College of Korean Medicine, Graduate School, Kyung Hee University) ;
  • Jung Min Kim (Department. of Biological Sciences in Korean Medicine, College of Korean Medicine, Graduate School, Kyung Hee University) ;
  • Seong Kyu Park (Department of Prescriptionology, College of Korean Medicine, Kyung Hee University)
  • 장문석 (경희대학교 한의과대학 처방제형학교실) ;
  • 방관석 (경희대학교 대학원 한의학과 처방제형학교실) ;
  • 이정진 (경희대학교 대학원 한의학과 처방제형학교실) ;
  • 허준영 (경희대학교 대학원 한의학과 처방제형학교실) ;
  • 김정민 (경희대학교 대학원 한의생명과학과 처방제형학교실) ;
  • 박성규 (경희대학교 한의과대학 처방제형학교실)
  • Received : 2024.11.15
  • Accepted : 2024.11.21
  • Published : 2024.11.30
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Abstract

Objectives : The purpose of this study was to investigate the antioxidant activity of water extract of Cistanches Herba (CH) in Leydig cells. Methods : We carried out the cytoprotective effect of CH in cultured mouse Leydig cells by MTT assay. Leydig cells treated with CH were incubated in the presence or absence of 100 μM hydrogen peroxide at 37℃ for 24 h. The protective effects of CH against hydrogen peroxide-induced oxidative stress, dihydrorhodamine 123 (DHR 123) assay, superoxide dismutase (SOD), catalase activity assays and lipid peroxide (LPO) were performed in Leydig TM3 cells. Results : Leydig cells treated with 5-250 ㎍/ml of CH showed no significant cytotoxicity. Leydig cells treated with 10 ㎍/ml of CH showed the most significant protective effect (49.0%, p < 0.05), decrease of intracellular levels of H2O2 (106.2%, p < 0.001) and decrease of LPO (1.87nmoles/mg protein, p < 0.05). Leydig cells treated with 10 ㎍/ml of CH showed increase of SOD activity and catalase activity but not significantly. Conclusions : These results suggest that CH has potent antioxidant activity on Leydig cells from H2O2-induced oxidative stress.

Keywords

References

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