Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Identification of Compounds in Lithospermum erythrorhizon Alcoholic Extract and Evaluation of Its Antioxidant Effect

자초 주정 추출물의 유효성분 분석 및 항산화 효능 평가

  • Ji Woong Heo (Departments of Veterinary Medicine, Gyeongsang National University) ;
  • Young Zoo You (Departments of Veterinary Medicine, Gyeongsang National University) ;
  • Min Jung Kim (Departments of Veterinary Medicine, Gyeongsang National University) ;
  • Ye Jin Yang (Departments of Veterinary Medicine, Gyeongsang National University) ;
  • Hu-Jang Lee (Departments of Veterinary Medicine, Gyeongsang National University) ;
  • Dong bin Lee (Departments of Veterinary Medicine, Gyeongsang National University) ;
  • Kwang Il Park (Departments of Veterinary Medicine, Gyeongsang National University)
  • 허지웅 (경상국립대학교 수의과대학) ;
  • 유영주 (경상국립대학교 수의과대학) ;
  • 김민정 (경상국립대학교 수의과대학) ;
  • 양예진 (경상국립대학교 수의과대학) ;
  • 이후장 (경상국립대학교 수의과대학) ;
  • 이동빈 (경상국립대학교 수의과대학) ;
  • 박광일 (경상국립대학교 수의과대학)
  • Received : 2024.11.13
  • Accepted : 2024.11.21
  • Published : 2024.11.30
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Abstract

Objectives: The overproduction and accumulation of free radicals and reactive oxygen species (ROS) result in oxidative stress at the cellular level, which play a critical role in the progression of numerous diseases. Plant-derived phenolic compounds are acknowledged for their substantial antioxidant activities, functioning as natural antioxidants within the organism. Therefore, the aim of this study was to identify bioactive compounds in the Lithospermum erythrorhizon alcoholic extract (LEAE) and to evaluate its antioxidant effect. Methods: Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to identify the active compounds in the LEAE that may contribute to its antioxidant effects. The antioxidant effect of LEAE was evaluated using DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) assays. To further assess the antioxidant capacity, the total phenolic and flavonoid contents were also measured. Results: LC-MS/MS analysis identified six predominant compounds in LEAE: Adenosine, Guanosine, Salicylic acid, Lithospermic acid, Salvianolic acid B and Salvianolic acid A. In the DPPH assay, significant free radical scavenging activity was observed starting from an LEAE concentration of 60 ㎍/mL. Similarly, the ABTS assay demonstrated a concentration-dependent reduction in free radical levels, beginning at an LEAE concentration of 20 ㎍/mL. Conclusions: LEAE exhibits a significant antioxidant effect, which is attributed to the presence of adenosine, guanosine, salicylic acid, lithospermic acid, salvianolic acid B, and salvianolic acid A. These findings suggest that LEAE may serve as a significant source of natural antioxidants and possess promise for treating disorders associated with oxidative stress.

Keywords

Acknowledgement

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2022R1I1A3053818).

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