The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Stem Rot of Pearl Millet Prevalence, Symptomatology, Disease Cycle, Disease Rating Scale and Pathogen Characterization in Pearl Millet-Klebsiella Pathosystem

  • Vinod Kumar Malik (Department of Plant Pathology, Chaudhary Charan Singh Haryana Agricultural University) ;
  • Pooja Sangwan (Department of Plant Pathology, Chaudhary Charan Singh Haryana Agricultural University) ;
  • Manjeet Singh (Department of Plant Pathology, Chaudhary Charan Singh Haryana Agricultural University) ;
  • Pavitra Kumari (Department of Plant Pathology, Chaudhary Charan Singh Haryana Agricultural University) ;
  • Niharika Shoeran (Department of Plant Pathology, Chaudhary Charan Singh Haryana Agricultural University) ;
  • Navjeet Ahalawat (Department of Bioinformatics and Computational Biology, Chaudhary Charan Singh Haryana Agricultural University) ;
  • Mukesh Kumar (Department of Genetics and Plant Breeding, Chaudhary Charan Singh Haryana Agricultural University) ;
  • Harsh Deep (Department of Genetics and Plant Breeding, Chaudhary Charan Singh Haryana Agricultural University) ;
  • Kamla Malik (Department of Microbiology, Chaudhary Charan Singh Haryana Agricultural University) ;
  • Preety Verma (Department of Plant Pathology, Chaudhary Charan Singh Haryana Agricultural University) ;
  • Pankaj Yadav (Department of Plant Pathology, Chaudhary Charan Singh Haryana Agricultural University) ;
  • Sheetal Kumari (Department of Plant Pathology, Chaudhary Charan Singh Haryana Agricultural University) ;
  • Aakash (Department of Plant Pathology, Chaudhary Charan Singh Haryana Agricultural University) ;
  • Sambandh Dhal (Department of Electrical and Computer Engineering, Texas A&M University)
  • Received : 2023.09.18
  • Accepted : 2023.12.29
  • Published : 2024.02.01
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Abstract

The oldest and most extensively cultivated form of millet, known as pearl millet (Pennisetum glaucum (L.) R. Br. Syn. Pennisetum americanum (L.) Leeke), is raised over 312.00 lakh hectares in Asian and African countries. India is regarded as the significant hotspot for pearl millet diversity. In the Indian state of Haryana, where pearl millet is grown, a new and catastrophic bacterial disease known as stem rot of pearl millet spurred by the bacterium Klebsiella aerogenes (formerly Enterobacter) was first observed during fall 2018. The disease appears in form of small to long streaks on leaves, lesions on stem, and slimy rot appearance of stem. The associated bacterium showed close resemblance to Klebsiella aerogenes that was confirmed by a molecular evaluation based on 16S rDNA and gyrA gene nucleotide sequences. The isolates were also identified to be Klebsiella aerogenes based on biochemical assays, where Klebsiella isolates differed in D-trehalose and succinate alkalisation tests. During fall 2021-2023, the disease has spread all the pearl millet-growing districts of the state, extending up to 70% disease incidence in the affected fields. The disease is causing considering grain as well as fodder losses. The proposed scale, consisting of six levels (0-5), is developed where scores 0, 1, 2, 3, 4, and 5 have been categorized as highly resistant, resistant, moderately resistant, moderately susceptible, susceptible, and highly susceptible disease reaction, respectively. The disease cycle, survival of pathogen, and possible losses have also been studied to understand other features of the disease.

Keywords

Acknowledgement

The authors are highly grateful to the Department of Plant Pathology, CCS Haryana Agricultural University, Hisar, Haryana, for providing experimental facilities to carry out this work.

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