The Plant Pathology Journal

한국식물병리학회 (The Korean Society of Plant Pathology)
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Screening of Antagonistic Bacillus against Brown Rot in Dendrocalamus latiflorus and Preparation of Applying Bacterial Suspension

  • Fengying Luo (College of Forestry, Sichuan Agricultural University) ;
  • Hang Chen (College of Forestry, Sichuan Agricultural University) ;
  • Wenjian Wei (College of Forestry, Sichuan Agricultural University) ;
  • Han Liu (Ganzi Institute of Forestry Research) ;
  • Youzhong Chen (Sichuan Guoguang Agrochemical Co., Ltd.) ;
  • Shujiang Li (College of Forestry, Sichuan Agricultural University)
  • 투고 : 2023.07.26
  • 심사 : 2023.11.28
  • 발행 : 2024.02.01
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초록

The aim of this study was to isolate biocontrol bacteria that could antagonize brown rot of Dendrocalamus latiflorus, optimize the culture conditions, and develop an effective biocontrol preparation for brown rot of D. latiflorus. This study isolated a bacterium with an antagonistic effect on bamboo brown rot from healthy D. latiflorus rhizosphere soil. Morphology, molecular biology, and physiological biochemistry methods identified it as Bacillus siamensis. The following culturing media and conditions improved the inhibition effect of B. siamensis: the best culturing media were 2% sucrose, 1.5% yeast extract, and 0.7% potassium chloride; the optimal culturing time, temperature, pH, and inoculation amount were 48 h, 30℃, 6, and 20%. The optimum formula of the applying bacterial suspension was 14% sodium dodecyl benzene sulfonate emulsifier, 4% Na2HPO4·2H2O, 0.3% hydroxypropyl methylcellulose thickener, and 20% B. siamensis. The pot experiment results showed the control effect of applying bacterial suspension, diluted 1,000 times is still better than that of 24% fenbuconazole suspension. The applying bacterial suspension enables reliable control of brown rot in D. latiflorus.

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과제정보

This research was supported by the National Natural Science Foundation of China (grant number 32171795) and the Sichuan Natural Science Foundation for Distinguished Young Scholar (grant number 2023NSFSC1936), and the Young Talents of Sichuan Tianfu Qingcheng Program.

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