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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Mitochondrial genome editing: strategies, challenges, and applications

  • Kayeong Lim (Brain Science Institute, Korea Institute of Science and Technology (KIST))
  • Received : 2023.11.10
  • Accepted : 2023.12.21
  • Published : 2024.01.31
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Abstract

Mitochondrial DNA (mtDNA), a multicopy genome found in mitochondria, is crucial for oxidative phosphorylation. Mutations in mtDNA can lead to severe mitochondrial dysfunction in tissues and organs with high energy demand. MtDNA mutations are closely associated with mitochondrial and age-related disease. To better understand the functional role of mtDNA and work toward developing therapeutics, it is essential to advance technology that is capable of manipulating the mitochondrial genome. This review discusses ongoing efforts in mitochondrial genome editing with mtDNA nucleases and base editors, including the tools, delivery strategies, and applications. Future advances in mitochondrial genome editing to address challenges regarding their efficiency and specificity can achieve the promise of therapeutic genome editing.

Keywords

Acknowledgement

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (RS-2023-00210965) and the Korea Institute of Science and Technology (KIST) Institutional Program (2E32161). Figures were created with BioRender.com.

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