Pediatric Gastroenterology, Hepatology & Nutrition

The Korean Society of Pediatric Gastroenterology, Hepatology and Nutrition (대한소아소화기영양학회)
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Identification of Autoantigens in Pediatric Gastric Juices

  • Hee-Shang Youn (Department of Pediatrics, Gyeongsang National University School of Medicine, Gyeongsang National University Hospital, Gyeongsang Institute of Health Science) ;
  • Jin-Su Jun (Department of Pediatrics, Gyeongsang National University School of Medicine, Gyeongsang National University Hospital, Gyeongsang Institute of Health Science) ;
  • Jung Sook Yeom (Department of Pediatrics, Gyeongsang National University School of Medicine, Gyeongsang National University Hospital, Gyeongsang Institute of Health Science) ;
  • Ji Sook Park (Department of Pediatrics, Gyeongsang National University School of Medicine, Gyeongsang National University Hospital, Gyeongsang Institute of Health Science) ;
  • Jae-Young Lim (Department of Pediatrics, Gyeongsang National University School of Medicine, Gyeongsang National University Hospital, Gyeongsang Institute of Health Science) ;
  • Hyang-Ok Woo (Department of Pediatrics, Gyeongsang National University School of Medicine, Gyeongsang National University Hospital, Gyeongsang Institute of Health Science) ;
  • Jung-Wook Yang (Department of Pathology, Gyeongsang National University School of Medicine, Gyeongsang Institute of Health Science) ;
  • Seung-Chul Baik (Department of Microbiology, Gyeongsang National University School of Medicine, Gyeongsang Institute of Health Science) ;
  • Woo-Kon Lee (Department of Microbiology, Gyeongsang National University School of Medicine, Gyeongsang Institute of Health Science) ;
  • Ji-Hyun Seo (Department of Pediatrics, Gyeongsang National University School of Medicine, Gyeongsang National University Hospital, Gyeongsang Institute of Health Science)
  • Received : 2022.07.21
  • Accepted : 2023.10.27
  • Published : 2024.01.15
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Abstract

Purpose: This study aimed to investigate the presence of autoantigens in the gastric juices of children. Methods: Gastric juice and serum samples were obtained from 53 children <15 years of age who underwent gastric endoscopy. Among these, 8, 22, and 23 participants were in the age groups 0-5, 6-10, and 11-15 years, respectively. These samples were analyzed using two-dimensional electrophoresis (2-DE), immunoblot analysis, and matrix-assisted laser desorption ionization-time of-flight mass spectrometry. Furthermore, we reviewed the histopathological findings and urease test results and compared them with the results of 2-DE and immunoblot analysis. Results: There were no statistically significant differences in urease test positivity, grades of chronic gastritis, active gastritis, or Helicobacter pylori infiltration of the antrum and body among the three age groups. Three distinct patterns of gastric juice were observed on 2-DE. Pattern I was the most common, and pattern III was not observed below the age of 5 years. Histopathological findings were significantly different among active gastritis (p=0.037) and H. pylori infiltration (p=0.060) in the gastric body. The immunoblots showed large spots at an approximate pH of 3-4 and molecular weights of 31-45 kDa. These distinct, large positive spots were identified as gastric lipase and pepsin A and C. Conclusion: Three enzymes, which are normally secreted under acidic conditions were identified as autoantigens. Further investigation of the pathophysiology and function of autoantigens in the stomach is required.

Keywords

Acknowledgement

The biospecimens and data used in this study were provided by the Biobank of Gyeongsang National University Hospital, a member of the Korea Biobank Network. The authors thank Kyung-Rang Park for the experimental procedures and data visualization.

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