Animal Bioscience

  • 제37권2호
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  • Pages.284-294
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  • 2024
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  • 2765-0189(pISSN)
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  • 2765-0235(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Apoptosis and autophagy of muscle cell during pork postmortem aging

  • Chunmei Li (College of Food Science and Engineering, Yangzhou University) ;
  • Xialian Yin (College of Food Science and Engineering, Yangzhou University) ;
  • Panpan Xue (Jiangsu Food & Pharmaceutical Science College) ;
  • Feng Wang (College of Food Science and Engineering, Yangzhou University) ;
  • Ruilong Song (Institute of Comparative Medicine, College of Veterinary Medicine, Yangzhou University) ;
  • Qi Song (College of Food Science and Engineering, Yangzhou University) ;
  • Jiamin Su (College of Food Science and Engineering, Yangzhou University) ;
  • Haifeng Zhang (College of Food Science and Engineering, Yangzhou University)
  • 투고 : 2023.04.19
  • 심사 : 2023.09.06
  • 발행 : 2024.02.01
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초록

Objective: Pork is an important source of animal protein in many countries. Subtle physiochemical changes occur during pork postmortem aging. The changes of apoptosis and autophagy in pork at 6 h to 72 h after slaughter were studied to provide evidence for pork quality. Methods: In this article, morphological changes of postmortem pork was observed through Hematoxylin-eosin staining, apoptotic nuclei were observed by TdT-mediated dUTP nick end labeling assay, protein related to apoptosis and autophagy expressions were tested by western blot and LC3 level were expressed according to immunofluorescence assay. Results: In this study, we found the occurrence of apoptosis in postmortem pork, and the process was characterized by nucleus condensation and fragmentation, formation of apoptotic bodies, increase in apoptosis-related Bax/Bcl-2 levels, and activation of caspases. Autophagy reached its peak between 24 and 48 h after slaughter, accompanied by the formation of autophagosomes on the cell membrane and expression of autophagy-related proteins beclin-1, P62, LC3-I, LC3-II, and ATG5. Conclusion: Obvious apoptosis was observed at 12 h and autophagy reached its peak at 48 h. The present work provides the evidence for the occurrence of apoptosis and autophagy during postmortem aging of pork. In conclusion, the apoptosis and autophagy of muscle cells discovered in this study have important implications for pork in the meat industry.

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과제정보

This research project was supported by grants from the National Natural Science Foundation of China (31800284) to Chunmei Li and the Joint Innovative, Scientific and Technological Research Program for Yangzhou City and Yangzhou University (YZ2020267) to Haifeng Zhang.

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