Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Fermentation characteristics and microbial community composition of wet brewer's grains and corn stover mixed silage prepared with cellulase and lactic acid bacteria supplementation

  • Guoqiang Zhao (Guangdong VTR Bio-Tech Co., Ltd.) ;
  • Hao Wu (State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University) ;
  • Yangyuan Li (Guangdong VTR Bio-Tech Co., Ltd.) ;
  • Li Li (Guangdong VTR Bio-Tech Co., Ltd.) ;
  • Jiajun He (Guangdong VTR Bio-Tech Co., Ltd.) ;
  • Xinjian Yang (Guangdong VTR Bio-Tech Co., Ltd.) ;
  • Xiangxue Xie (Guangdong VTR Bio-Tech Co., Ltd.)
  • Received : 2023.05.11
  • Accepted : 2023.06.26
  • Published : 2024.01.01
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Abstract

Objective: The objective of this study was to investigate how cellulase or/and lactic acid bacteria (LAB) affected the fermentation characteristic and microbial community in wet brewer's grains (WBG) and corn stover (CS) mixed silage. Methods: The WBG was mixed thoroughly with the CS at 7:3 (w/w). Four treatment groups were studied: i) CON, no additives; ii) CEL, added cellulase (120 U/g fresh matter [FM]), iii) LAB, added LAB (2×106 cfu/g FM), and iv) CLA, added cellulase (120 U/g FM) and LAB (2×106 cfu/g FM). Results: All additive-treated groups showed higher fermentation quality over the 30 d ensiling period. As these groups exhibited higher (p<0.05) LAB counts and lactic acid (LA) content, along with lower pH value and ammonia-nitrogen (NH3-N) content than the control. Specifically, cellulase-treated groups (CEL and CLA) showed lower (p<0.05) neutral detergent fiber and acid detergent fiber contents than other groups. All additives increased the abundance of beneficial bacteria (Firmicutes, Lactiplantibacillus, and Limosilactobacillus) while they decreased abundance of Proteobacteria and microbial diversity as well. Conclusion: The combined application of cellulase and LAB could effectively improve the fermentation quality and microbial community of the WBG and CS mixed silage.

Keywords

Acknowledgement

This study was supported by a general program of the National Natural Science Foundation of China (Grant Number. 31972593), and the National Key Research Development Program of China (Grant No. 2022YFC2805105).

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