Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Isolation and Characterization of 𝛽-Glucosidase-Producing Yeast, Rhodotorula sp. GYP-1

𝛽-Glucosidase 생성 효모 Rhodotorula sp. GYP-1의 분리 및 특성

  • Hyun-Soo Roh (Department of Microbial Biotechnology, College of Mokwon University) ;
  • Min-Young Kwon (Department of Microbial Biotechnology, College of Mokwon University) ;
  • Sol-Bi Kim (SOLBIO Co., LTD) ;
  • Jae-Eun Cho (SOLBIO Co., LTD) ;
  • Song-Ih Han (Department of Microbial Biotechnology, College of Mokwon University)
  • 노현수 (목원대학교 미생물생명공학과) ;
  • 권민영 (목원대학교 미생물생명공학과) ;
  • 김솔비 (쏠바이오(주)) ;
  • 조재은 (쏠바이오(주)) ;
  • 한송이 (목원대학교 미생물생명공학과)
  • Received : 2023.08.23
  • Accepted : 2023.10.30
  • Published : 2023.10.31
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Abstract

Nine microbial strains were isolated from the byproduct of ginseng processing and field of ginseng cultivation. Two strains among them were confirmed. Phylogenetic analysis of these 𝛽-Glucosidase strains confirmed that strain GYP-1 belongs to the Rhodotorula and strain GYP-3-3 belong to genus Brachybacterium. Rhodotorula sp. GYP-1 was finally selected due to its high biomass production. The 𝛽-Glucosidase activity of Rhodotorula sp. GPY-1 was assessed at 30 ℃, and Higher than 70% of the enzyme activity was maintained at the temperature range of 20-40℃. Although the optimum pH for the highest enzyme activity was pH 5.0, the enzyme was stable throughout the pH range of 5.0-8.0. In addition, Rhodotorula sp. demonstrated antifungal activity against the ginseng root rot disease caused by Botrytis.

인삼부산물 및 인삼밭 토양에서 분리한 9균주 중 𝛽-Glucosidase 생성 균주 GYP-1과 GYP-3-3 균주를 선발하였다. 선발된 𝛽-Glucosidase 생성 균주에 대하여 16S rRNA 유전자 염기서열과 ITS 염기서열을 기반으로 계통 분석을 실시한 결과, GYP-1 균주는 Rhodotorula 속에 속하며, GYP-3-3은 Brachybacterium 속에 속하는 것으로 확인되었다. 특히, Rhodotorula sp. GYP-1 균주는 호기성 효모종으로 biomass 생산량이 높아 최종 우수 균주로 선발하였다. Rhodotorula sp. GYP-1가 생성하는 𝛽-Glucosidase의 온도 및 pH에 따른 효소 활성 및 안정성을 검정한 결과, 30 ℃에서 6.7 unit/ml로 가장 높은 활성을 나타내었고, 20 ℃ ~ 40 ℃에서 효소 활성의 약 70 % 이상을 유지하는 것으로 확인하였다. pH에 따른 효소의 활성 및 안정의 경우, pH 5에서 6.8 unit/ml으로 가장 높은 활성을 나타내었고 pH 5~pH 8까지 93.3 % 이상의 효소 활성을 유지하는 것으로 확인하였다. Rhodotorula sp. GYP-1가 생성하는 𝛽-Glucosidase는 ginsenoside Rb1 minor 진세노사이드로 분해하는 것으로 확인되었다. 또한, 인삼 뿌리 병원균(Botrytis cinerea)에 대해 항진균능을 갖는 것으로 확인되었다.

Keywords

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