The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Effects of Tetrapanax papyrifer stem and Akebiae quinata stem on a rat model of monosodium iodoacetate-induced osteoarthritis

통초(通草)와 목통(木通) 추출물이 monosodium iodoacetate(MIA)로 유발된 골관절염 동물 모델에 미치는 효과

  • Sang Nam Lee (Dept. of Qigong, College of Korean Medicine, Daegu Haany University) ;
  • Bu-Il Seo (Dept. of Herbology, College of Korean Medicine, Daegu Haany University)
  • 이상남 (대구한의대학교 한의과대학 기공학교실) ;
  • 서부일 (대구한의대학교 한의과대학 본초학교실)
  • Received : 2023.10.12
  • Accepted : 2023.11.25
  • Published : 2023.11.30
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Abstract

Objectives : This study was planned to evaluate the therapeutic effectiveness and possible underlying mechanism of TPE (Tetrapanax papyrifer stem(inner part of the stem Extract) and AQE (Akebiae quinata stem Extract) on osteoarthritis. Methods : Osteoarthritis models were induced through intra-articular injection of MIA (monosodium iodoacetate) 50 μL with 80 mg/ml in rats. Excluding the normal group, Osteoarthritis-induced rats were divided into 4 groups (Control, INDO, TPE, AQE). The drug concentrations were indomethacin 5 mg/kg, TPE 200 mg/kg, and AQE 200 mg/kg, and were orally administered once a day for a couple of weeks. After drug supplementation, the effects of TPE and AQE were measured with serum diagnosis, western blotting, and histopathological staining. Results : It was found that the DPPH and ABTS free radical erasure ability of AQE was better than that of TPE. AQE administration improved rear limb overload and it led to relieving pain. Both PTE and AQE significantly reduced the expression of inflammatory mediators COX-2, iNOS, and inflammatory cytokine IL-1β and IL-6 by inhibiting the phosphorylation of IκBα and deactivating the pathway of NF-κBp65. On the other hand, TNF-α was significantly reduced only by administration of AQE. In addition, histopathological analysis showed that the administration of AQE compared to PTE suppressed cartilage degeneration and effectively suppressed damage to proteoglycan, a component of ECM. Conclusion : Reviewing these experimental results, TPE and AQE possessed the effect of delaying the progress of osteoarthritis and protecting cartilage. In addition, the results of this study show that AQE has a better cartilage protection effect than TPE.

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References

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