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The Korean Society of Phycology (한국조류학회(藻類))
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Isolation and cultivation of freshwater diatom Nitzschia palea HY1 for increasing biomass and fucoxanthin production

  • Hyunji Won (Department of Environmental Science, Hanyang University) ;
  • Eunmi Ro (Department of Life Science, Research Institute for Natural Sciences, Hanyang University) ;
  • Seungbeom Seo (Department of Life Science, Research Institute for Natural Sciences, Hanyang University) ;
  • Baik-Ho Kim (Department of Environmental Science, Hanyang University) ;
  • EonSeon Jin (Department of Environmental Science, Hanyang University)
  • Received : 2023.06.29
  • Accepted : 2023.09.03
  • Published : 2023.09.15
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Abstract

Diatoms, a type of microalgae distributed worldwide, have been identified as potential sources of biomass, lipids, and high-value compounds. While marine diatoms have been extensively studied, the potential of freshwater diatoms still needs to be explored. In this study, a novel strain of freshwater diatom was isolated from the Jungnangcheon stream located in Seoul, Republic of Korea (37°33'08.0" N, 127°02'40.0" E). This newly isolated strain was classified through phylogenetic analysis, and its morphology was investigated using light and electron microscopy; it was named Nitzschia palea HY1. N. palea HY1 grown in freshwater media (FDM) produced higher biomass (0.68 g L-1) and fucoxanthin production (9.19 mg L-1) than in conventional diatom media. Furthermore, increasing the bicarbonate concentration from 2 to 10 mM enhanced the maximum biomass and fucoxanthin production in FDM by 2.7 fold and 1.5 fold, respectively. Remarkably, the introduction of aeration to the modified FDM (MFDM) led to a substantial increase in the maximum biomass and fucoxanthin production of N. palea HY1, exhibiting 3.8-fold and 4.1-fold enhancement, respectively, compared to FDM alone. These findings suggest that optimizing the cultivation of N. palea HY1 using MFDM could provide an alternative to marine sources for fucoxanthin production.

Keywords

Acknowledgement

This research was supported by Korea Environmental Industry & Technology Institute (KEITI) through "The Project to develop eco-friendly new materials and processing technology derived from wildlife," funded by the Korea Ministry of Environment (MOE) (2021003270007).

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