Mycobiology

The Korean Society of Mycology (한국균학회)
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Didymella acutilobae sp. nov. Causing Leaf Spot and Stem Rot in Angelica acutiloba

  • Gyo-Bin Lee (Global Agro-Consulting Corporation) ;
  • Ki Deok Kim (Laboratory of Plant Disease and Biocontrol, College of Life Sciences and Biotechnology, Korea University) ;
  • Weon-Dae Cho (Global Agro-Consulting Corporation) ;
  • Wan-Gyu Kim (Global Agro-Consulting Corporation)
  • Received : 2023.07.31
  • Accepted : 2023.08.28
  • Published : 2023.10.31
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Abstract

During disease surveys of Angelica acutiloba plants in Korea, leaf spot symptoms were observed in a field in Andong in July 2019, and stem rot symptoms in vinyl greenhouses in Yangpyeong in April 2020. Incidence of leaf spot and stem rot of the plants ranged from 10 to 20% and 5 to 30%, respectively. Morphological and cultural characteristics of fungal isolates from the leaf spot and stem rot symptoms fitted into those of the genus Phoma. Molecular phylogenetic analyses of two single-spore isolates from the symptoms using concatenated sequences of LSU, ITS, TUB2, and RPB2 genes authenticated an independent cluster from other Didymella (anamorph: Phoma) species. Moreover, the isolates showed different morphological and cultural characteristics in comparison to closely related Didymella species. These discoveries confirmed the novelty of the isolates. Pathogenicity of the novel Didymella species isolates was substantiated on leaves and stems of A. acutiloba through artificial inoculation. Thus, this study reveals that Didymella acutilobae sp. nov. causes leaf spot and stem rot in Angelica acutiloba.

Keywords

Acknowledgement

This study was supported by a research grant (PJ014507012020) from the Rural Development Administration, Korea.

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