DOI QR코드

DOI QR Code

Unveiling the Diversity of Hydnum in the Republic of Korea with One New Species, Hydnum paucispinum

  • Ji Seon Kim (School of Biological Sciences and Institute of Microbiology, Seoul National University) ;
  • Wonjun Lee (School of Biological Sciences and Institute of Microbiology, Seoul National University) ;
  • Changmu Kim (Species Diversity Research Division, National Institute of Biological Resources) ;
  • Hanna Park (School of Biological Sciences and Institute of Microbiology, Seoul National University) ;
  • Chang Sun Kim (Forest Biodiversity Division, Korea National Arboretum) ;
  • Young Woon Lim (School of Biological Sciences and Institute of Microbiology, Seoul National University)
  • 투고 : 2023.07.12
  • 심사 : 2023.09.26
  • 발행 : 2023.10.31

초록

Hydnum is a genus of ectomycorrhizal fungi belonging to the Hydnaceae family. It is widely distributed across different regions of the world, including North America, Europe, and Asia; however, some of them showed disjunct distributions. In recent years, with the integration of molecular techniques, the taxonomy and classification of Hydnum have undergone several revisions and advancements. However, these changes have not yet been applied in the Republic of Korea. In this study, we conducted an integrated analysis combining the morphological and molecular analyses of 30 specimens collected over a period of approximately 10 years in the Republic of Korea. For molecular analysis, the sequence data of the internal transcribed spacer (ITS) region, the large subunit of nuclear ribosomal RNA gene (nrLSU), and a portion of translation elongation factor 1-a (TEF1) were employed as molecular markers. Through this study, we identified eight species that had previously not been reported to occur in the Republic of Korea, including one new species, Hydnum paucispinum. A taxonomic key and detailed descriptions of the eight Hydnum species are provided in this study.

키워드

과제정보

We would like to thank Editage (www.editage.co.kr) for English language editing.

참고문헌

  1. Miller LW. The genera of Hydnaceae. Mycologia. 1933;25(4):286-302. doi: 10.1080/00275514.1933.12020669.
  2. Moncalvo JM, Nilsson RH, Koster B, et al. The cantharelloid clade: dealing with incongruent gene trees and phylogenetic reconstruction methods. Mycologia. 2006;98(6):937-948. doi: 10.3852/mycologia.98.6.937.
  3. Petersen RH. The typification of Hydnum L. ex Fries. Taxon. 1973;22(1):99-104. doi: 10.2307/1218039.
  4. Donk M. The generic names proposed for Hymenomycetes. V, "Hydnaceae" (continuation). Taxon. 1956;5(5):95-115. doi: 10.2307/1216240.
  5. Maas Geesteranus R. Hydnaceous fungi of the Eastern old world. Vehr K Ned Akad Wet. 1971;II.60:1-176.
  6. Niskanen T, Liimatainen K, Nuytinck J, et al. Identifying and naming the currently known diversity of the genus Hydnum, with an emphasis on European and North American taxa. Mycologia. 2018;110(5):890-918. doi: 10.1080/00275514.2018.1477004.
  7. Diamandis S, Perlerou C. The mycoflora of the chestnut ecosystems in Greece. For Snow and Landscape Res. 2001;76(3):499-504.
  8. Raidl S, Agerer R. Studien an ektomykorrhizen. XLII: ontogenie der rhizomorphen von Laccaria amethystina, Hydnum rufescens und Sarcodon imbricatus. Nova Hedwigia. 1992;55(3-4):279-307.
  9. Sugawara R, Sotome K, Maekawa N, et al. Mycorrhizal synthesis, morpho-anatomical characterization of mycorrhizae, and evaluation of mycorrhiza-forming ability of Hydnum albidum-like species using monokaryotic and dikaryotic cultures. Mycorrhiza. 2021;31(3):349-359. doi: 10.1007/s00572-021-01024-7.
  10. Harrison KA. New or little known North American stipitate Hydnums. Can J Bot. 1964;42(9):1205-1233. doi: 10.1139/b64-116.
  11. Maas Geesteranus RA. Notes on hydnums. Persoonia-Molecular Phylogeny and Evolution of Fungi. 1960;1(3):341-384.
  12. Murrill WA. Additions to Florida fungi-V. Vol. 67(4), In: Bulletin of the Torrey Botanical Club; New York (NY): Torrey Botanical Society; 1940. p. 275-281. doi: 10.2307/2481174.
  13. Grigoriev IV, Cullen D, Goodwin SB, et al. Fueling the future with fungal genomics. Mycology. 2011;2(3):192-209.
  14. Hibbett DS, Pine EM, Langer E, et al. Evolution of gilled mushrooms and puffballs inferred from ribosomal DNA sequences. Proc Natl Acad Sci USA. 1997;94(22):12002-12006. doi: 10.1073/pnas.94.22.12002.
  15. Baird RE. Type studies of North American and other related taxa-of stipitate Hydnums. In: Bibliotheca Mycologica; 1986. ISBN 978-3-443-59004-8. [AQ]
  16. Larsson KH, Svantesson S, Miscevic D, et al. Reassessment of the generic limits for Hydnellum and Sarcodon (Thelephorales, Basidiomycota). MycoKeys. 2019;54:31-47. doi: 10.3897/mycokeys.54.35386.
  17. Feng B, Wang X-H, Ratkowsky D, et al. Multilocus phylogenetic analyses reveal unexpected abundant diversity and significant disjunct distribution pattern of the hedgehog mushrooms (Hydnum L.). Sci Rep. 2016;6(1):25586. doi: 10.1038/srep25586.
  18. Swenie RA, Baroni TJ, Matheny PB. Six new species and reports of Hydnum (Cantharellales) from Eastern North America. MycoKeys. 2018;42(42):35-72. doi: 10.3897/mycokeys.42.27369.
  19. Cao T, Hu Y-P, Yu J-R, et al. A phylogenetic overview of the Hydnaceae (Cantharellales, Basidiomycota) with new taxa from China. Stud Mycol. 2021;99(1):100121-100121. https://doi.org/10.1016/j.simyco.2021.100121
  20. Huhtinen S, Ruotsalainen J. Variability of Hydnum rufescens in Finland: three taxa hidden under one name-and appearance? Karstenia. 2006;46(1):17-24. doi: 10.29203/ka.2006.412.
  21. Ostrow H, Beenken L. Hydnum ellipsosporum spec. nov. (Basidiomycetes, Cantharellales)-ein doppelganger von Hydnum rufescens Fr. Zeitschrift Fuer Mykologie. 2004;70(2):137-156.
  22. Vizzini A, Picillo B, Ercole E, et al. Detecting the variability of Hydnum ovoideisporum (Agaricomycetes, Cantharellales) on the basis of Italian collections, and H. magnorufescens sp. nov. Mycosphere. 2012;4(1):32-44. doi: 10.5943/mycosphere/4/1/2.
  23. NIBR. National Institute of Biological Resources, 2022; [cited 2022 Mar 23]. Available from: https://kbr.go.kr/
  24. Kaburagi Y. Korea forest experiment station. Korean and Manchurian practical manual of forest. Tokyo: Yokendo; 1940. p. 339-367.
  25. Cho HJ, Park MS, Lee H, et al. A systematic revision of the ectomycorrhizal genus Laccaria from Korea. Mycologia. 2018;110(5):948-961. https://doi.org/10.1080/00275514.2018.1507542
  26. Lee H, Wissitrassameewong K, Park MS, et al. Taxonomic revision of the genus Lactarius (Russulales, Basidiomycota) in Korea. Fungal Divers. 2019;95(1):275-335. doi: 10.1007/s13225-019-00425-6.
  27. Wisitrassameewong K, Looney BP, Le HT, et al. Lactarius subgenus Russularia (Basidiomycota, Russulales): novel Asian species, worldwide phylogeny and evolutionary relationships. Fungal Biol. 2016;120(12):1554-1581. doi: 10.1016/j.funbio.2016.08.004.
  28. Kornerup A, Wanscher J. Methuen handbook of colour. London: Eyre Methuen Ltd; 1978. ISBN 978-0413334008.
  29. Collins TJ. ImageJ for microscopy. Biotechniques. 2007;43(1 Suppl):25-30. doi: 10.2144/000112517.
  30. Snell WH, Dick EA. A glossary of mycology. Cambridge (MA): Harvard University Press; 1971. ISBN 0674-35451-6.
  31. Largent DL, Stuntz DE. How to identify mushrooms to genus. 1973. ISBN: 978-0916422004.
  32. Gardes M, Bruns TD. ITS primers with enhanced specificity for basidiomycetes-application to the identification of mycorrhizae and rusts. Mol Ecol. 1993;2(2):113-118. doi: 10.1111/j.1365-294x.1993.tb00005.x.
  33. White TJ, Bruns TD, Lee SB, et al. Analysis of phylogenetic relationships by amplification and direct sequencing of ribosomal RNA genes. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, editors. PCR protocols: a guide to methods and applications. New York: Academic Press; 1990. p. 315-322.
  34. Sugawara R, Maekawa N, Sotome K, et al. Systematic revision of Hydnum species in Japan. Mycologia. 2022;114(2):413-452. doi: 10.1080/00275514.2021.2024407.
  35. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol. 2016;33(7):1870-1874. doi: 10.1093/molbev/msw054.
  36. Katoh K, Misawa K, Kuma K, et al. MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Res. 2002;30(14):3059-3066. doi: 10.1093/nar/gkf436.
  37. Stamatakis A. RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics. 2006;22(21):2688-2690. doi: 10.1093/bioinformatics/btl446.
  38. Yanaga K, Sotome K, Ushijima S, et al. Hydnum species producing whitish basidiomata in Japan. Mycoscience. 2015;56(4):434-442. doi: 10.1016/j.myc.2015.01.001.
  39. Linnaeus C. Species plantarum, exhibentes plantas rite cognitas ad genera relatas, cum differentiis specificis, nominibus trivialibus, synonymis selectis, locis natalibus, secundum systema sexuale digestas, Impensis GC Nauk; 1797. doi: 10.5962/bhl.title.37656.
  40. van der Linde S, Suz LM, Orme CDL, et al. Environment and host as large-scale controls of ectomycorrhizal fungi. Nature. 2018;558(7709):243-248. doi: 10.1038/s41586-018-0189-9.
  41. Yang H, Zang Y, Yuan Y, et al. Selectivity by host plants affects the distribution of arbuscular mycorrhizal fungi: evidence from ITS rDNA sequence metadata. BMC Evol Biol. 2012;12(1):50. doi: 10.1186/1471-2148-12-50.
  42. Cho HJ, Lee H, Park MS, et al. Two new species of Laccaria (Agaricales, Basidiomycota) from Korea. Mycobiology. 2020;48(4):288-295. https://doi.org/10.1080/12298093.2020.1786961
  43. Wilson AW, May TW, Mueller GM. Biogeography of the ectomycorrhizal mushroom genus Laccaria. In: Tedersoo L, editor. Biogeography of mycorrhizal symbiosis. ecological studies, Vol. 230. Cham: Springer; 2017. doi: 10.1007/978-3-319-56363-3_13.
  44. Wu BW, Gao C, Chen L, et al. Host phylogeny is a major determinant of Fagaceae-associated ectomycorrhizal fungal community assembly at a regional scale. Front Microbiol. 2018;9:2409. doi: 10.3389/fmicb.2018.02409.
  45. Phukhamsakda C, Nilsson RH, Bhunjun CS, et al. The numbers of fungi: contributions from traditional taxonomic studies and challenges of meta-barcoding. Fungal Diver. 2022;114(1):327-386. doi: 10.1007/s13225-022-00502-3.