Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Effects of grape seed proanthocyanidin extract on side effects of high-dose methylprednisolone administration in male rats

  • Aslihan Sur (Department of Veterinary Medicine, Vocational School of Kepsut, Balikesir University) ;
  • Seda Ifazoglu Mutlu (Department of Animal Nutrition and Nutritional Diseases, Faculty of Veterinary Medicine, Firat University) ;
  • Pinar Tatli Seven (Department of Animal Nutrition and Nutritional Diseases, Faculty of Veterinary Medicine, Firat University) ;
  • Ismail Seven (Department of Plant and Animal Production, Vocational School of Sivrice, Firat University) ;
  • Abdullah Aslan (Department of Biology, Faculty of Science, Firat University) ;
  • Meltem Kizil (Department of Physiology, Faculty of Veterinary Medicine, Firat University) ;
  • Recai Kulaksiz (Department of Reproduction and Artifcial Insemination, Faculty of Veterinary Medicine, Balikesir University) ;
  • Mustafa Hilmi Yaranoglu (Experimental Research Center, Balikesir University) ;
  • Selim Esen (Balikesir Directorate of Provincial Agriculture and Forestry, Republic of Turkey Ministry of Agriculture and Forestry)
  • Received : 2023.01.13
  • Accepted : 2023.05.30
  • Published : 2023.10.15
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Abstract

In this study, we investigated the effects of grape seed proanthocyanidin extract (GSPE) against the side effects of high-dose administration of methylprednisolone (MP) in male rats. A total of 32 adult Wistar male albino rats were divided into four groups: (1) control (CON), received standard food only; (2) MP, received standard food +intraperitoneal injection of 60 mg/kg MP on day 7; (3) GSPE, received standard food+200 mg/kg/day GSPE; and (4) MP+GSPE, received standard food+200 mg/kg/day of GSPE+intraperitoneal injection of 60 mg/kg MP on day 7. All animals in the GSPE and GSPE+MP groups were treated once a day by oral gavage for 14 consecutive days. The feed intake of rats in the MP and MP+GSPE groups decreased significantly by 24.14% and 13.52%, respectively (p<0.05). Administration of MP resulted in significant increases in serum concentrations of blood urea nitrogen (p<0.001), glucose (p<0.01), alkaline phosphatase, and adrenocorticotropic hormone (p<0.05). High-dose MP administration significantly reduced catalase (p<0.001) and glutathione peroxidase (p<0.05) concentrations in the liver and kidney tissues of rats, while glutathione concentrations were only reduced in liver tissue (p<0.05). The expression levels of Bcl-2 and TNF-α in liver, kidney, and testicular tissue were significantly increased, while the expression levels of caspase-3 were reduced (p<0.001). Furthermore, sperm concentration was significantly affected by GSPE in rats induced by high-dose MP, and sperm loss was significantly reduced in MP+GSPE (p<0.05). These findings suggest that GSPE could be useful as a supplement to alleviate MP-induced toxicity in rats.

Keywords

Acknowledgement

This research was funded by the Directorate of Scientific Research Projects of Balikesir University, grant number 2020/005.

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