Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Association of immunotoxicological indices with lung cancer biomarkers in poultry, grape, and rose farming workers

  • Anju Maharjan (Department of Occupational Health, College of Bio and Medical Sciences, Daegu Catholic University) ;
  • Ravi Gautam (Department of Occupational Health, College of Bio and Medical Sciences, Daegu Catholic University) ;
  • Manju Acharya (Department of Occupational Health, College of Bio and Medical Sciences, Daegu Catholic University) ;
  • JiHun Jo (Department of Occupational Health, College of Bio and Medical Sciences, Daegu Catholic University) ;
  • DaEun Lee (Department of Occupational Health, College of Bio and Medical Sciences, Daegu Catholic University) ;
  • Pramod Bahadur K C (Graduate School Department of Toxicology, Daegu Catholic University) ;
  • Young‑A Lee (Department of Food Science and Nutrition, College of Bio and Medical Sciences, Daegu Catholic University) ;
  • Jung‑Taek Kwon (Environmental Health Research Department, National Institute of Environmental Research) ;
  • HyoCher Kim (Rural Development Administration, National Institute of Agricultural Sciences) ;
  • KyungRan Kim (Rural Development Administration, National Institute of Agricultural Sciences) ;
  • ChangYul Kim (Graduate School Department of Toxicology, Daegu Catholic University) ;
  • HyoungAh Kim (Department of Preventive Medicine, College of Medicine, The Catholic University of Korea) ;
  • Yong Heo (Department of Occupational Health, College of Bio and Medical Sciences, Daegu Catholic University)
  • Received : 2023.03.23
  • Accepted : 2023.06.18
  • Published : 2023.10.15
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Abstract

Exposure to occupational hazards like dust, pesticides, diesel emission particles, or physical hazards in the agricultural sector is known to cause adverse health effects on farm workers. Our study aimed at addressing the association of immunomodulatory status with plasma levels of lung cancer biomarkers in farming population, attempting to recognition of vulnerable farming group. Blood samples from apparently healthy 51 chicken husbandry, 19 grape orchard, and 21 rose greenhouse workers were subjected to evaluate plasma levels of two representative lung cancer biomarkers, pro-gastrin releasing peptide (Pro-GRP) and cytokeratin fragment 19 (CYFRA 21-1). Peripheral blood mononuclear cells obtained from farmers were used for natural killer (NK) cell phenotyping and cytokines (interferon-gamma, IFN-γ and interleukin-13, IL-13) profiling in the culture supernatant. Compared to the rose greenhouse farmers, the grape orchard and chicken husbandry workers revealed a significantly upregulated plasma Pro-GRP and CYFRA 21-1 level. A low proportion of NK cells was observed among the female grape orchard workers and a lowered IFN-γ:IL-13 ratio was seen in the grape and chicken husbandry workers than the rose workers. Our findings imply that grape orchard and chicken husbandry workers have more disturbed immune homeostasis implicated with augmentation in the levels of lung cancer biomarkers than the rose greenhouse workers.

Keywords

Acknowledgement

The authors disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was supported by the research grant from Daegu Catholic University (Grant no. 20201212).

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