Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Growth of Intestinal Bacteria and Intestinal Inflammation of Sprout Extract from Common Buckwheat and Tartary Buckwheat

일반메밀과 쓴메밀의 새싹 추출물의 장내 유익균 증식 및 염증조절 효능 평가

  • Su Jeong Kim (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration) ;
  • Hwang Bae Sohn (Technology Cooperation Bureau, Rural Development Administration) ;
  • Jong Won Kim (R&D Center, Cell Biotech Co., Ltd.) ;
  • Sanghyun Lim (R&D Center, Cell Biotech Co., Ltd.) ;
  • Jong Nam Lee (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration) ;
  • Su Hyoung Park (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration) ;
  • Jung Hwan Nam (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration) ;
  • Do Yeon Kim (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration) ;
  • Ye Jin Lee (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration) ;
  • Dong Chil Chang (Planning & Coordination Division, National Institute of Crop Science, Rural Development Administration) ;
  • Yul Ho Kim (Crop Cultivation and Environment Research Division, National Institute of Crop Science, Rural Development Administration)
  • 김수정 (농촌진흥청 국립식량과학원 고령지농업연구소) ;
  • 손황배 (농촌진흥청 기술협력국 수출농업지원과) ;
  • 김종원 ((주)쎌바이오텍 세포공학연구소) ;
  • 임상현 ((주)쎌바이오텍 세포공학연구소) ;
  • 이종남 (농촌진흥청 국립식량과학원 고령지농업연구소) ;
  • 박수형 (농촌진흥청 국립식량과학원 고령지농업연구소) ;
  • 남정환 (농촌진흥청 국립식량과학원 고령지농업연구소) ;
  • 김도연 (농촌진흥청 국립식량과학원 고령지농업연구소) ;
  • 이예진 (농촌진흥청 국립식량과학원 고령지농업연구소) ;
  • 장동칠 (농촌진흥청 국립식량과학원 기획조정과) ;
  • 김율호 (농촌진흥청 국립식량과학원 재배환경과)
  • Received : 2023.09.06
  • Accepted : 2023.09.18
  • Published : 2023.10.01
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Abstract

We aimed to assess the potential growth-promoting effects of buckwheat sprout on intestinal bacteria and their anti-inflammation effects in a cellular model of intestinal inflammation. The growth of Bifidobacterium longum ssp. infantis BT1 was enhanced with the addition of the sprout extract of tartary buckwheat. Further, in the inflammatory model cells cultured with Raw 264.7 cells were treated with buckwheat sprout including each 10 probiotics before the addition of lipopolysaccharide (LPS) to induce inflammation in Raw 264.7 cells. Buckwheat sprout in both Bifidobacterium longum ssp. infantis BT1 and Lacticaseibacillus paracasei LPC5 significantly reduced the production of NO and PGE2. The above results indicate that buckwheat sprout extract which contains with various physiologically active substances such as rutin, quercetin, and choline is effective in suppressing NO and PGE2 production, which are inflammation-related indicators. The present study suggests that buckwheat sprout could induce positive effects on the intestinal beneficial bacteria and in anti-inflammation.

본 연구에서는 메밀 새싹 추출물의 비피더스균과 락토바실러스균 증식에 미치는 영향, 항염증 효과 및 관련 생리활성 물질의 분석을 통해 프리바이오틱스 소재로서의 활용 가능성을 평가하였다. 메밀 새싹 추출물을 10종의 프로바이오틱스에 첨가하여 각각 배양하였을 때, 대조군보다 pH 값이 낮아지고, 생균수는 증가하였다. 쓴메밀 새싹 추출물을 Bifidobacterium longum ssp. infantis BT1에 첨가하여 배양한 실험군의 생균수는 8.61×108 CFU/mL로 증식 효과가 가장 뛰어났다. 메밀 새싹 추출물을 10종의 프로바이오틱스에 첨가한 다음 LPS로 유도하였을 때, 세포독성에 대한 보호 효과와 NO, PGE2 발현에서도 유의적인 억제 효과를 보여 우수하였다. 특히, 비피더스균 중 BT1에서 NO 생성량이 29.5 mM로 가장 낮았으며, 락토바실러스균 중에서는 Lacticaseibacillus paracasei LPC5에서 30.6 mM로 NO 생성량이 매우 낮아 항염증 효과가 있었다. 이상의 결과는 루틴, 퀘세틴 및 콜린 등 다양한 생리활성 물질을 가진 메밀 새싹 추출물이 특정 배양액에서 염증 관련 지표인 NO 생성 및 PGE2 생성량 억제에 효과가 있음을 나타내는 것으로 메밀이 장내 유익균 증식과 염증 조절에 긍정적인 영향을 줄 수 있을 것으로 판단된다.

Keywords

Acknowledgement

본 연구는 농촌진흥청 국립식량과학원 작물시험연구사업(연구과제: 고품질 다용도 메밀 품종 개발 과제_2단계, 과제번호 PJ01606801)의 지원에 의해 이루어진 결과로 이에 감사드립니다.

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