Korean Chemical Engineering Research

  • 제61권4호
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  • Pages.576-583
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  • 2023
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  • 0304-128X(pISSN)
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  • 2233-9558(eISSN)
한국화학공학회 (The Korean Institute of Chemical Engineers)
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탄소나노튜브로 개질된 탄소종이 기반 젖산산화효소 - 카탈레이즈 전극 제작 및 특성 분석

Fabrication and Characterization of Carbon Nanotube-modified Carbon Paper-based Lactate Oxidase-catalase Electrode

  • 시키 (경상국립대학교 화학공학과 및 그린에너지 연구소) ;
  • 셀바라잔 바르시니 (경상국립대학교 화학공학과 및 그린에너지 연구소) ;
  • 양영일 (경상국립대학교 반도체공학과) ;
  • 김혁한 (단국대학교 과학기술대학 화학과) ;
  • 김창준 (경상국립대학교 화학공학과 및 그린에너지 연구소)
  • Ke Shi (Department of Chemical Engineering and RIGET) ;
  • Varshini Selvarajan (Department of Chemical Engineering and RIGET) ;
  • Yeong-Yil Yang (Department of Semiconductor Engineering, Gyeongsang National University) ;
  • Hyug-Han Kim (Department of Chemistry, Dankook University) ;
  • Chang-Joon Kim (Department of Chemical Engineering and RIGET)
  • 투고 : 2023.08.10
  • 심사 : 2023.09.25
  • 발행 : 2023.11.01
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초록

본 연구에서는 전극의 전기 전도도 증대와 젖산 산화반응의 부산물인 과산화수소 생성 완화가 젖산 산화효소 전극 성능에 미치는 영향을 조사하였다. 유연성 있는 탄소종이 표면을 단일벽 탄소나노튜브로 개질하여 전극의 전기 전도도를 향상시켰다. 카탈라아제를 도입하여 젖산 산화반응에서 발생하는 과산화수소를 제거하였다. 젖산 산화효소와 카탈라아제가 동시에 고정화된 탄소종이 전극은 젖산 산화효소만 고정화된 탄소종이 전극보다 1.7배 많은 전류를 생성하였다. 단일벽 탄소나노튜브로 개질된 탄소종이 표면에 젖산 산화효소와 카탈라아제가 동시에 고정화된 전극은 171 µA의 전류를 생산하였는데, 이는 탄소종이 표면에 젖산 산화효소만 고정화된 전극이 생산하는 전류보다 2배 높은 값이다. 최적화된 전극은 젖산 농도가 20 mM까지 선형반응을 보여 센서용 전극으로 사용 가능함을 확인하였다.

This study aimed to investigate the impact of enhancing the electrode conductivity and mitigating the production of hydrogen peroxide - a by-product arising from lactate oxidation - on the performance of lactate electrodes. The electrical conductivity of the electrode was improved by modifying the surface of carbon paper with single-walled carbon nanotubes. Catalase was introduced to effectively eliminate the hydrogen peroxide produced during the lactate oxidation reaction. The carbon paper electrode, with simultaneous immobilization of both lactate oxidase and catalase, yielded a current 1.7 times greater than the electrode where only lactate oxidase was immobilized. The electrode in which lactate oxidase and catalase were co-immobilized on the surface of carbon paper modified with single-walled carbon nanotubes, produced a current of 171 µA, which was more than twice as much current as the carbon paper with only lactate oxidase immobilized. The optimized electrode showed a linear response up to lactate concentration of 20 mM, confirming that it can be used as a sensor electrode.

키워드

과제정보

이 성과는 정부의 재원으로 한국연구재단의 지원을 받아 수행된 연구임(NRF-2020R1F1A1054433, 2021RIS-003).

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