Journal of Microbiology and Biotechnology

  • 제33권10호
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  • Pages.1361-1369
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  • 2023
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Crystal Structures of 6-Phosphogluconate Dehydrogenase from Corynebacterium glutamicum

  • Hyeonjeong Yu (School of Life Sciences, BK21 FOUR KNU Creative BioResearch Group, Kyungpook National University) ;
  • Jiyeon Hong (KNU Institute for Microorganisms, Kyungpook National University) ;
  • Jihye Seok (School of Life Sciences, BK21 FOUR KNU Creative BioResearch Group, Kyungpook National University) ;
  • Young-Bae Seu (School of Life Sciences, BK21 FOUR KNU Creative BioResearch Group, Kyungpook National University) ;
  • Il-Kwon Kim (KNU Institute for Microorganisms, Kyungpook National University) ;
  • Kyung-Jin Kim (School of Life Sciences, BK21 FOUR KNU Creative BioResearch Group, Kyungpook National University)
  • 투고 : 2023.05.04
  • 심사 : 2023.06.16
  • 발행 : 2023.10.28
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초록

Corynebacterium glutamicum (C. glutamicum) has been considered a very important and meaningful industrial microorganism for the production of amino acids worldwide. To produce amino acids, cells require nicotinamide adenine dinucleotide phosphate (NADPH), which is a biological reducing agent. The pentose phosphate pathway (PPP) can supply NADPH in cells via the 6-phosphogluconate dehydrogenase (6PGD) enzyme, which is an oxidoreductase that converts 6-phosphogluconate (6PG) to ribulose 5-phosphate (Ru5P), to produce NADPH. In this study, we identified the crystal structure of 6PGD_apo and 6PGD_NADP from C. glutamicum ATCC 13032 (Cg6PGD) and reported our biological research based on this structure. We identified the substrate binding site and co-factor binding site of Cg6PGD, which are crucial for understanding this enzyme. Based on the findings of our research, Cg6PGD is expected to be used as a NADPH resource in the food industry and as a drug target in the pharmaceutical industry.

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과제정보

This work was supported by the Cooperative Research Program for Agricultural Science & Technology Development (Project No. PJ01492602), Rural Development Administration, Republic of Korea. And this work is further supported by the Development of next-generation biorefinery platform technologies for leading bio-based chemicals industry project (2022M3J5A1056072) and by Development of platform technologies of microbial cell factories for the next-generation biorefineries project (2022M3J5A1056117) from National Research Foundation supported by the Korean Ministry of Science and ICT.

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