Biomolecules & Therapeutics

  • 제31권6호
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  • Pages.619-628
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  • 2023
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  • 1976-9148(pISSN)
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  • 2005-4483(eISSN)
한국응용약물학회 (The Korean Society of Applied Pharmacology)
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Effect of Prunetin on Streptozotocin-Induced Diabetic Nephropathy in Rats - a Biochemical and Molecular Approach

  • Jose Vinoth Raja Antony Samy (Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University) ;
  • Nirubama Kumar (Department of Biochemistry, Kongunadu Arts and Science College) ;
  • Sengottuvelu Singaravel (Department of Pharmacology, Nandha College of Pharmacy) ;
  • Rajapandiyan Krishnamoorthy (Department of Food Science and Nutrition, College of Food and Agriculture Sciences, King Saud University) ;
  • Mohammad A Alshuniaber (Department of Food Science and Nutrition, College of Food and Agriculture Sciences, King Saud University) ;
  • Mansour K. Gatasheh (Department of Biochemistry, College of Science, King Saud University) ;
  • Amalan Venkatesan (Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University) ;
  • Vijayakumar Natesan (Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University) ;
  • Sung-Jin Kim (Department of Pharmacology and Toxicology, Metabolic Diseases Research Laboratory, School of Dentistry, Kyung Hee University)
  • 투고 : 2023.03.27
  • 심사 : 2023.07.30
  • 발행 : 2023.11.01
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초록

In the modern era, chronic kidney failure due to diabetes has spread across the globe. Prunetin (PRU), a component of herbal medicines, has a broad variety of pharmacological activities; these may help to slow the onset of diabetic kidney disease. The anti-nephropathic effects of PRU have not yet been reported. The present study explored the potential nephroprotective actions of PRU in diabetic rats. For 28 days, nephropathic rats were given oral doses of PRU (20, 40, and 80 mg/kg). Body weight, blood urea, creatinine, total protein, lipid profile, liver marker enzymes, carbohydrate metabolic enzymes, C-reactive protein, antioxidants, lipid peroxidative indicators, and the expression of insulin receptor substrate 1 (IRS-1) and glucose transporter 2 (GLUT-2) mRNA genes were all examined. Histological examinations of the kidneys, liver, and pancreas were also performed. The oral treatment of PRU drastically lowered the blood glucose, HbA1c, blood urea, creatinine, serum glutamic-oxaloacetic transaminase, serum glutamic pyruvic transaminase, alkaline phosphatase, lipid profile, and hexokinase. Meanwhile, the levels of fructose 1,6-bisphosphatase, glucose-6-phosphatase, and phosphoenol pyruvate carboxykinase were all elevated, but glucose-6-phosphate dehydrogenase dropped significantly. Inflammatory marker antioxidants and lipid peroxidative markers were also less persistent due to this administration. PRU upregulated the IRS-1 and GLUT-2 gene expression in the nephropathic group. The possible renoprotective properties of PRU were validated by histopathology of the liver, kidney, and pancreatic tissues. It is therefore proposed that PRU (80 mg/kg) has considerable renoprotective benefits in diabetic nephropathy in rats.

키워드

과제정보

The authors acknowledge the support from the Researchers supporting project number (RSP2023R393), King Saud University, Riyadh, Saudi Arabia.

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