Animal Bioscience

  • 제36권12호
  • /
  • Pages.1821-1830
  • /
  • 2023
  • /
  • 2765-0189(pISSN)
  • /
  • 2765-0235(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
권호 표지
DOI QR코드

DOI QR Code

Seminal plasma modulates post-thaw longevity and motility of frozen sperm in dromedary camel

  • 투고 : 2023.04.12
  • 심사 : 2023.07.19
  • 발행 : 2023.12.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Objective: This study investigated the effect of adding seminal plasma to frozen-thawed semen on the quality of sperm and pregnancy following insemination in dromedary camels. Methods: In experiment 1, the frozen-thawed semen from 9 collections (3 bulls) was further diluted with either the base extender or homologous seminal plasma (HSP). In the second experiment, a pooled sample of frozen-thawed semen was diluted with either seminal plasma from another three bulls. Live percentage, total and progressive motility, functional and acrosome integrity, and sperm kinematics were evaluated at 15, 60, and 120 minutes post-thawing and compared to the non-treated control. In experiment 3, frozen semen was used to inseminate camels in the following experimental groups: 1-Single insemination with double dose undiluted frozen semen (n = 9); 2-Re-insemination in 6 hours with undiluted semen (n = 13); 3-Single insemination with HSP treated sperm (n = 14). Results: Frozen-thawed sperm diluted in HSP or the non-homologous seminal plasma from Bull C indicated an improvement in all parameters after 1 hour post-thawing incubation (p<0.05). The proportion of total and progressively motile sperm did not drop significantly at 60 minutes post-thawing when diluted with the seminal plasma of Bull C (p>0.05). Double insemination with nontreated sperm and single insemination with HSP-treated sperm resulted in similar pregnancy rates (15.3% vs 21.4%, p>0.05). None of the camels conceived with double-dose single insemination of nontreated sperm. Conclusion: Seminal plasma improves sperm longevity and motility after thawing in dromedary camel with a significant between-bull variation in effect. Low post-thaw sperm longevity might be the cause behind the low pregnancy rates in frozen semen insemination of dromedary camels.

키워드

과제정보

The authors are grateful for the support of Zabeel Office for H.H. Sheikh Mohammad Bin Rashid, Prime Minister of UAE and the Ruler of Dubai. This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

참고문헌

  1. Rodriguez-Martinez H, Tienthai P, Suzuki K, Funahashi H, Ekwall H, Johannisson A. Involvement of oviduct in sperm capacitation and oocyte development in pigs. Reprod Suppl 2001;58:129-45. 
  2. Bjorndahl L, Kvist U. Human sperm chromatin stabilization: a proposed model including zinc bridges. Mol Hum Reprod 2010;16:23-9. https://doi.org/10.1093/molehr/gap099 
  3. Rodriguez-Martinez H, Saravia F, Wallgren M, et al. Boar spermatozoa in the oviduct. Theriogenology 2005;63:514-35. https://doi.org/10.1016/j.theriogenology.2004.09.028 
  4. du Fosse NA, Lashley EELO, Anholts JDH, et al. Impaired immunomodulatory effects of seminal plasma may play a role in unexplained recurrent pregnancy loss: Results of an in vitro study. J Reprod Immunol 2022;151:103500. https://doi.org/10.1016/j.jri.2022.103500 
  5. Bromfield JJ. A role for seminal plasma in modulating pregnancy outcomes in domestic species. Reproduction 2016;152:R223-R32. https://doi.org/10.1530/rep-16-0313 
  6. Bromfield JJ, Schjenken JE, Chin PY, Care AS, Jasper MJ, Robertson SA. Maternal tract factors contribute to paternal seminal fluid impact on metabolic phenotype in offspring. Proc Natl Acad Sci USA 2014;111:2200-5. https://doi.org/10.1073/pnas.1305609111 
  7. Crean AJ, Dwyer JM, Marshall DJ. Fertilization is not a new beginning: the relationship between sperm longevity and offspring performance. PloS one 2012;7:e49167. https://doi.org/10.1371/journal.pone.0049167 
  8. Juyena NS, Stelletta C. Seminal plasma: an essential attribute to spermatozoa. J Androl 2012;33:536-51. https://doi.org/10.2164/jandrol.110.012583 
  9. Rodriguez-Martinez H, Kvist U, Ernerudh J, Sanz L, Calvete JJ. Seminal plasma proteins: what role do they play? Am J Reprod Immunol 2011;66:11-22. https://doi.org/10.1111/j.1600-0897.2011.01033.x 
  10. Duncan MW, Thompson HS. Proteomics of semen and its constituents. Proteomics Clin Appl 2007;1:861-75. https://doi.org/10.1002/prca.200700228 
  11. Kershaw-Young CM, Maxwell WMC. Seminal plasma components in camelids and comparisons with other species. Reprod Domest Anim 2012;47:369-75. https://doi.org/10.1111/j.1439-0531.2012.02100.x 
  12. Pini T, Leahy T, de Graaf SP. Sublethal sperm freezing damage: manifestations and solutions. Theriogenology 2018;118:172-81. https://doi.org/10.1016/j.theriogenology.2018.06.006 
  13. Loomis PR, Graham JK. Commercial semen freezing: Individual male variation in cryosurvival and the response of stallion sperm to customized freezing protocols. Anim Reprod Sci 2008;105:119-28. https://doi.org/10.1016/j.anireprosci.2007.11.010 
  14. Mendeluk G, Gonzalez Flecha FL, Castello PR, Bregni C. Factors involved in the biochemical etiology of human seminal plasma hyperviscosity. J Androl 2000;21:262-7. https://doi.org/10.1002/j.1939-4640.2000.tb02104.x 
  15. Deen A, Vyas S, Sahani MS. Semen collection, cryopreservation and artificial insemination in the dromedary camel. Anim Reprod Sci 2003;77:223-33. https://doi.org/10.1016/s0378-4320(03)00040-x 
  16. Skidmore JA, Malo CM, Crichton EG, Morrell JM, Pukazhenthi BS. An update on semen collection, preservation and artificial insemination in the dromedary camel (Camelus dromedarius). Anim Reprod Sci 2018;194:11-8. https://doi.org/10.1016/j.anireprosci.2018.03.013 
  17. Kareskoski AM, Reilas T, Andersson M, Katila T. Motility and plasma membrane integrity of spermatozoa in fractionated stallion ejaculates after storage. Reprod Domest Anim 2006;41:33-8. https://doi.org/10.1111/j.1439-0531.2006.00647.x 
  18. Kareskoski M, Sankari S, Johannisson A, Kindahl H, Andersson M, Katila T. The Association of the presence of seminal plasma and its components with sperm longevity in fractionated stallion ejaculates. Reprod Domest Anim 2011;46:1073-81. https://doi.org/10.1111/j.1439-0531.2011.01789.x 
  19. Garcia JC, Dominguez JC, Pena FJ, et al. Thawing boar semen in the presence of seminal plasma: Effects on sperm quality and fertility. Anim Reprod Sci. 2010;119:160-5. https://doi.org/10.1016/j.anireprosci.2009.11.001 
  20. Maxwell WMC, Evans G, Mortimer ST, Gillan L, Gellatly ES, McPhie CA. Normal fertility in ewes after cervical insemination with frozen-thawed spermatozoa supplemented with seminal plasma. Reprod Fertil Dev 1999;11:123-6. https://doi.org/10.1071/rd99046 
  21. Okazaki T, Abe S, Yoshida S, Shimada M. Seminal plasma damages sperm during cryopreservation, but its presence during thawing improves semen quality and conception rates in boars with poor post-thaw semen quality. Theriogenology 2009;71:491-8. https://doi.org/10.1016/j.theriogenology.2008.08.014 
  22. Diana Fanelli DVM, Tesi M, Monaco D, et al. Deep-horn artificial insemination with frozen thawed semen after re-extension in autologous seminal plasma may improve pregnancy rates in jennies. J Equine Vet Sci 2022;112:103932. https://doi.org/10.1016/j.jevs.2022.103932 
  23. Bravo PW, Alarcon V, Baca L, et al. Semen preservation and artificial insemination in domesticated South American camelids. Anim Reprod Sci 2013;136:157-63. https://doi.org/10.1016/j.anireprosci.2012.10.005 
  24. Malo C, Grundin J, Morrell JM, Skidmore JA. Individual male dependent improvement in post-thaw dromedary camel sperm quality after addition of catalase. Anim Reprod Sci 2019;209:106168. https://doi.org/10.1016/j.anireprosci.2019.106168 
  25. Pillet E, Duchamp G, Batellier F, et al. Egg yolk plasma can replace egg yolk in stallion freezing extenders. Theriogenology 2011;75:105-14. https://doi.org/10.1016/j.theriogenology.2010.07.015 
  26. Aalseth EP, Saacke RG. Vital staining and acrosomal evaluation of bovine sperm. Gamete Res 1986;15:73-81. https://doi.org/10.1002/mrd.1120150108 
  27. Prochowska S, Nizanski W, Fontbonne A. Hypo-osmotic swelling test (HOST) for feline spermatozoa: the simplified procedure and the aspect of sperm morphology. Animals 2022;12:903. https://doi.org/10.3390/ani12070903 
  28. Seyedasgari F, Asadi B, Sebastyen S, Guillen R. Identification of sperm motility subpopulations in Gyr falcon (Falco rusticolus) ejaculate: a tool for investigating between subject variation. J Anim Reprod Biotechnol 2022;37:193-201. https://doi.org/10.12750/JARB.37.3.193 
  29. Asadi B, Seyedasgari F, Ashrafi Tamai I, et al. Isolated bacteria from the uteri of camels with different reproductive backgrounds: a study on sampling methodology, prevalence, and clinical significance. Vet Sci 2023;10:39. https://doi.org/10.3390/vetsci10010039 
  30. de Andrade A, Zaffalon F, Celeghini E, et al. Addition of seminal plasma to post-thawing equine semen: what is the effect on sperm cell viability? Reprod Domest Anim 2011;46:682-6. https://doi.org/10.1111/j.1439-0531.2010.01729.x 
  31. Rodriguez-Martinez H, Martinez EA, Calvete JJ, Pena Vega FJ, Roca J. Seminal plasma: relevant for fertility? Int J Mol Sci 2021;22:4368. https://doi.org/10.3390/ijms22094368 
  32. Bubenickova F, Postlerova P, Simonik O, Sirohi J, Sichtar J. Effect of seminal plasma protein fractions on stallion sperm cryopreservation. Int J Mol Sci 2020;21:6415. https://doi.org/10.3390/ijms21176415 
  33. Centurion F, Vazquez JM, Calvete JJ, et al. Influence of porcine spermadhesins on the susceptibility of boar spermatozoa to high dilution. Biol Reprod 2003;69:640-6. https://doi.org/10.1095/biolreprod.103.016527 
  34. Morrell JM, Georgakas A, Lundeheim N, Nash D, Davies Morel MCG, Johannisson A. Effect of heterologous and homologous seminal plasma on stallion sperm quality. Theriogenology 2014;82:176-83. https://doi.org/10.1016/j.theriogenology.2014.03.020 
  35. Al-Essawe E, Tsikis G, Labas V, et al. Seminal plasma components differ between "good freezer" and "poor freezer" stallions. Anim Reprod Sci 2016;169:113. https://doi.org/10.1016/j.anireprosci.2016.03.047 
  36. Aller JF, Rebuffi GE, Cancino AK, Alberio RH. Influence of cryopreservation on the motility, viability and fertility of llama (Lama Glama) spermatozoa. Arch Zootec 2003;52:15-23. 
  37. Miller CD. Optimizing the use of frozen-thawed equine semen. Theriogenology 2008;70:463-8. https://doi.org/10.1016/j.theriogenology.2008.04.037 
  38. Ahmadi H, Csabai T, Gorgey E, Rashidiani S, Parhizkar F, Aghebati-Maleki L. Composition and effects of seminal plasma in the female reproductive tracts on implantation of human embryos. Biomed Pharmacother 2022;151:113065. https://doi.org/10.1016/j.biopha.2022.113065 
  39. Ortiz WG, Rizo JA, Carvalheira LR, et al. Effects of intrauterine infusion of seminal plasma at artificial insemination on fertility of lactating Holstein cows. J Dairy Sci 2019;102:6587-94. https://doi.org/10.3168/jds.2019-16251 
  40. Portus BJ, Reilas T, Katila T. Effect of seminal plasma on uterine inflammation, contractility and pregnancy rates in mares. Equine Vet J 2005;37:515-9. https://doi.org/10.2746/042516405775314844