Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Effect of enzymolytic soybean meal supplementation on performance, nitrogen excretion, serum biochemical parameters and intestinal morphology in broilers fed low-protein diets

  • Xin Zhu (College of Animal Science and Veterinary Medicine, Shenyang Agricultural University) ;
  • Kai Gao (College of Animal Science and Veterinary Medicine, Shenyang Agricultural University) ;
  • Ziyi Zhang (College of Animal Science and Veterinary Medicine, Shenyang Agricultural University) ;
  • Haiying Liu (College of Animal Science and Veterinary Medicine, Shenyang Agricultural University) ;
  • Guiqin Yang (College of Animal Science and Veterinary Medicine, Shenyang Agricultural University)
  • Received : 2023.03.16
  • Accepted : 2023.06.14
  • Published : 2023.11.01
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Abstract

Objective: The objective of this study was to investigate the effect of supplementation with enzymolytic soybean meal (ESBM) on broilers fed low crude protein (CP) diets. Methods: A total of 360 one-day-old broilers were randomly assigned to six treatments with 6 replicates per treatment and 10 chicks per replicate for a period of 42 days. Chicks were fed a basal standard high-CP diet as a positive control (PC), a low-CP diet (reducing 10 g/kg CP from the PC) as a negative control (NC), or an NC + 0.5%, 1.0%, 1.5%, or 2.0% ESBM diet. Results: Compared to chicks fed the PC, chicks fed the NC had a decreased body weight gain (BWG, p<0.05) from 1 to 42 days, but supplementation with 2.0% ESBM restored BWG (p<0.05) and even linearly improved the feed conversion rate (FCR, p<0.05). Digestibility of CP and ether extract was increased (p<0.05) in chicks fed a 1.0% ESBM diet compared to the PC. With increasing levels of ESBM, nitrogen (N) excretion decreased (p<0.05). The addition of ESBM to the diet did not affect (p>0.05) serum concentrations of total protein, albumin and total cholesterol but led to a descending trend in triglycerides and an ascending trend in calcium and urea N at 42 days (p<0.10). There were no differences (p>0.05) in villus height (VH), crypt depth (CD), and VH/CD (V/C) of the duodenum and jejunum between the PC and NC at both 21 days and 42 days, while increasing dietary ESBM levels linearly (p<0.05) decreased CD and increased V/C of the duodenum and jejunum at both 21 days and 42 days. Conclusion: The findings indicated that ESBM could be used in broiler low-CP diets to improve production performance, decrease N excretion, and enhance intestinal health.

Keywords

Acknowledgement

The authors are grateful for the support by the National Natural Science Foundation of China (No. 31772618) and Liaoning Revitalization Talents Project (XLYC1902024).

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