대한화장품학회지 (Journal of the Society of Cosmetic Scientists of Korea)

  • 제49권3호
  • /
  • Pages.285-290
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  • 2023
  • /
  • 1226-2587(pISSN)
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  • 2288-9507(eISSN)
대한화장품학회 (Society of Cosmetic Scientists of Korea)
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NF-κB 활성 조절을 통한 Lactobacillus johnsonii 파쇄액의 항염 효과

Anti-inflammatory Effects of Lactobacillus johnsonii Lysate via Regulation of NF-κB Activity

  • 차화준 (오산대학교, 뷰티코스메틱계열)
  • Hwa Jun Cha (Osan university, Department of Beauty & Cosmetics, Osan University)
  • 투고 : 2023.08.29
  • 심사 : 2023.09.27
  • 발행 : 2023.09.30
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Abstract

본 연구에서는 김치유래의 Lactobacillus johnsonii의 anti-inflammation 효능을 확인하였다. Rat 유래 대식세포인 Raw 264.7세포에 Lactobacillus johnsonii 파쇄물을 처리하여 염증유발 marker인 TNFα와 IL1β의 발현량을 확인한 결과 250 ㎍/mL 추출물을 처리시 1 ㎍/mL LPS를 처리한 대조군 대비 각각 40.55%와 34.66%의 TNFα와 IL1β의 발현량 감소를 확인하였다. 또한 LPS에 의한 cytokine 발현의 핵심 전사인자인 NF-κB의 전사활성을 확인한결과 1 ㎍/mL LPS를 처리한 대조군 대비 NF-κB의 전사활성은 40.76% 억제됨을 확인하였다. 때문에 본 연구결과를 통해 Lactobacillus johnsonii 파쇄물은 LPS에 의한 cytokine의 발현을 방지 및 NF-κB전사활성 조절을 통해 항염 또는 피부진정 기능성 소재로써 가능성을 확인하였다.

In this study, the anti-inflammation efficacy of Lactobacillus johnsonii derived from Kimchi was investigated. Raw 264.7 cells, which are rat-derived macrophages, were treated with Lactobacillus johnsonii lysate to confirm the expression level of TNFα and IL1β, which are inflammatory markers, and when treating 250 ㎍/mL extract, the expression level of TNFα and IL1β decreased by 40.55% and 34.66% compared to the control group treated with 1 ㎍/mL LP, respectively. In addition, as a result of confirming the transcriptional activity of NF-κB, a key transcription factor in cytokine expression by LPS, it was confirmed that the transcriptional activity of NF-κB was 40.76% inhibited compared to the control group treated with 1 ㎍/mL LPS. Therefore, the results of this study confirmed that Lactobacillus johnsonii lysate is likely to be an anti-inflammatory or skin-soothing functional material by preventing the expression of cytokine by LPS and controlling NF-κB transcriptional activity.

Keywords

Acknowledgement

본 과제(결과물)는 교육부와 한국연구재단의 재원으로 지원을 받아 수행된 3단계 산학연협력 선도전문대학 육성사업(LINC 3.0)의 연구결과입니다.

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