International Journal of Industrial Entomology and Biomaterials

  • 제47권1호
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  • Pages.1-11
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  • 2023
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  • 3022-7542(pISSN)
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  • 2586-4785(eISSN)
한국잠사학회 (Korean Society of Sericultural Science)
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Molecular methods for diagnosis of microbial pathogens in muga silkworm, Antheraea assamensis Helfer (Lepidoptera: Saturniidae)

  • Gangavarapu Subrahmanyam (Seri-biotech Research Laboratory, Central Silk Board, Ministry of Textiles: Govt. of India) ;
  • Kangayam M. Ponnuvel (Seri-biotech Research Laboratory, Central Silk Board, Ministry of Textiles: Govt. of India) ;
  • Kallare P Arunkumar (Silkworm Division, Central Muga Eri Research and Training Institute (CMER&TI), Central Silk Board, Ministry of Textiles: Govt. of India) ;
  • Kamidi Rahul (Central Sericultural Research & Training Institute, Central Silk Board, Ministry of Textiles: Govt. of India) ;
  • S. Manthira Moorthy (National Silkworm Seed Organization (NSSO), Central Silk Board, Ministry of Textiles: Govt. of India) ;
  • Vankadara Sivaprasad (Seri-biotech Research Laboratory, Central Silk Board, Ministry of Textiles: Govt. of India)
  • 투고 : 2023.06.16
  • 심사 : 2023.07.24
  • 발행 : 2023.09.30
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초록

The Indian golden muga silkworm, Antheraea assamensis Helfer is an economically important wild silkworm endemic to Northeastern part of India. In recent years, climate change has posed a threat to muga silk production due to the requirement that larvae be reared outdoors. Since the muga silkworm larvae are exposed to the vagaries of nature, the changing climate has increased the incidence of microbial diseases in the rearing fields. Accurate diagnosis of the disease causing pathogens and its associated epidemiology are prerequisites to manage the diseases in the rearing field. Although conventional microbial culturing methods are widely used to identify pathogenic bacteria, they would not provide meaningful information on a wide variety of silkworm pathogens. The information on use of molecular diagnostic tools in detection of microbial pathogens of wild silk moths is very limited. A wide range of molecular and immunodiagnostic techniques including denaturing gradient gel electrophoresis (DGGE), random amplified polymorphism (RAPD), 16S rRNA/ITSA gene sequencing, multiplex polymerase chain reaction (M-PCR), fluorescence in situ hybridization (FISH), immunofluorescence, and repetitive-element PCR (Rep-PCR), have been used for detecting and characterizing the pathogens of insects with economic significance. Nevertheless, the application of these molecular tools for detecting and typing entomopathogens in surveillance studies of muga silkworm rearing is very limited. Here, we discuss the possible application of these molecular techniques, their advantages and major limitations. These methods show promise in better management of diseases in muga ecosystem.

키워드

과제정보

The authors are thankful to Central Silk Board, Ministry of Textiles, Govt. of India for the financial support in projects ARP5878 and ARP08007MI.

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