Journal of Convergence Korean Medicine (대한융합한의학회지)

Academy of Convergence Korean Medicine (대한융합한의학회)
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Effect of TJGB on the liver of high-fat diet-fed mice and the viability of HepG2 cells

고지방식이를 급여한 마우스의 간과 HepG2 세포에서 TJGB의 효과에 대한 연구

  • Hee-Young Kim (Department of Integrated Drug Development and Natural Products, Graduate School, Kyung Hee University) ;
  • Yea-Jin Park (Department of Rehabilitative Medicine of Korean Medicine and Neuropsychiatry, College of Korean Medicine, Sangji University) ;
  • Hyo-Jin An (Department of Integrated Drug Development and Natural Products, Graduate School, Kyung Hee University)
  • 김희영 (경희대학교 약학대학 천연물융합신약개발학과) ;
  • 박예진 (상지대학교 한의과대학 한방재활의학과) ;
  • 안효진 (경희대학교 약학대학 천연물융합신약개발학과)
  • Received : 2023.06.07
  • Accepted : 2023.06.29
  • Published : 2023.06.30
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Abstract

Objectives: This study was performed to investigate the effect of TJGB on the liver of high-fat diet (HFD)-fed mice and the cell viability of HepG2 cells. Methods: After a week adaptation, 8-week-old C57BL/6N mice were fed with a 45% HFD or normal diet for 3 weeks. For the next 9 weeks, the mice were divided into 6 groups: normal diet group; HFD group; HFD plus orlistat group; HFD plus Ephedra sinica Stapf (ES) group; HFD plus low dose of TJGB group; HFD plus high dose of TJGB group. To estimate the effect of TJGB in the liver of HFD-fed mice, the protein expressions of phospho-acetyl-CoA carboxylase (p-ACC) and liver X Receptor (LXR) were determined by Western blot assay. The cell viability of ES and TJG was also evaluated in HepG2 cells. Results: The administration of TJGB had little effect on the protein expressions of p-ACC and LXR in the liver of HFD-fed mice. And the cytotoxicity was showed above 7.8 ㎍/mL in HepG2 cells. Conclusion: Further research is needed to evaluate the mechanism of TJGB on hepatic steatosis and cytotoxicity in HepG2 cells.

Keywords

References

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