DOI QR코드

DOI QR Code

Electrochemical Sensing of Hydrogen Peroxide Using Prussian Blue@poly(p-phenylenediamine) Coated Multi-walled Carbon Nanotubes

  • Young-Eun Jeon (Department of Chemistry Education, Seoul National University) ;
  • Wonhyeong Jang (Department of Chemistry Education, Seoul National University) ;
  • Gyeong-Geon Lee (Department of Chemistry Education, Seoul National University) ;
  • Hun-Gi Hong (Department of Chemistry Education, Seoul National University)
  • 투고 : 2023.06.12
  • 심사 : 2023.08.10
  • 발행 : 2023.10.20

초록

In this study, a nanocomposite of multi-walled carbon nanotubes@poly(p-phenylenediamine)-Prussian blue (MWCNTs@PpPD-PB) was synthesized and employed for the electrochemical detection of hydrogen peroxide (H2O2). A straightforward approach was utilized to prepare an electrochemical H2O2 sensor using a MWCNTs@PpPD-PB modified glassy carbon electrode, and its electrochemical behavior was investigated through techniques such as electrochemical impedance spectroscopy, cyclic voltammetry, and amperometry. The modified electrode displayed a favorable electrocatalytic response towards the reduction of H2O2 in an acidic solution. The developed sensor exhibited linearity in the concentration range of 0.005 mM to 2.225 mM for H2O2, with high sensitivity (583.6 ㎂ mM-1cm-2) and a low detection limit (0.95 ㎛, S/N = 3) at an applied potential of +0.15 V (vs. Ag/AgCl). Additionally, the sensor demonstrated excellent selectivity, reproducibility, and stability. Moreover, successful detection of H2O2 was achieved in real samples.

키워드

INTRODUCTION

The analysis of hydrogen peroxide (H2O2) holds significant importance in a wide range of fields. H2O2 serves as a commonly employed oxidizing agent, making it an essential mediator in the realms of biology, food, medicine, industry, and the environment.1-2 Methods such as chemiluminescence,3 fluorescence,4 spectrophotometry,5 and electrochemical techniques have been reported for the accurate detection of H2O2 concentration. The accuracy of the detection of H2O2 has been increased with the development of detection techniques for each area, and the electrochemical sensors in particular play more important roles because they have operational simplicity, low cost, high sensitivity, and suitability for real-time detection.6

Here, Prussian blue (PB) having an open, zeolite-like structure is well known as the prototype of polynuclear transition-metal hexacyanometalates.7 Due to the structure of PB, large molecules cannot penetrate into the lattice whereas small molecules such as H2O2 can be.8 PB exhibits electrochemical,9-10 electrochromic,11-12 photophysical,13 and magnetic properties.14-15 In particular, the electrocatalytic property of PB enables the potential development of electrochemical sensors using the PB-modified electrode.16-17 Consequently, the pursuit of a reliable H2O2 detection method using PB complexes as catalysts has gained significant attention.18-20

The first study on the synthesis of PB film was reported by Neff,21 and various methods have been used to fabricate different PB-based hybrids for PB-modified electrodes so far.22-23

To improve the sensitivity and stability of PB-based electrochemical sensors, several methods have been suggested to synthesize PB nanoparticles (PBNPs) over a range of sizes and shapes by chemical processes such as reverse micelles,24 ionic liquids,25-26 mesoporous silicate,27 and polymer beads as mediums or templates.28 Furthermore, the synthesis of PBNPs using functionalized organic polymers has been widely studied. These polymers provide the chemical and spatial environment, allowing for the stable construction of particles. They also help to form a colloid and prevent the solution from aggregation.29-31 Research on conductive polymers has continued owing to their excellent physicochemical properties resulting from their unique π-conjugated system32 which provides a powerful platform for sensing applications and a variety of functions from the polymers.

Poly(p-phenylenediamine) (PpPD) is one of the most studied multifunctional poly-arylamines owing to the characteristics of the enhanced redox activity by the presence of amino groups in its chain of PpPD. Because of the low conductivity of PpPD, the use of a conductive inorganic material such as carbon nanotubes,33 noble metals,34 or nanoparticles35 has been studied to make PpPD available for electrolysis or electrochemical sensors. In particular, multi-walled carbon nanotubes (MWCNTs) which are composed of π-conjugated networks, have high electrical conductivity, excellent thermal stability, and large surface area, and thus the advantage of easy functionalization or the manipulation of their surface properties.36-37

In this study, MWCNTs coated with PpPD conductive polymer based on the PBNPs were proposed for the development of an electrochemical H2O2 sensor. The MWCNTs@PpPD was synthesized by simply coating PpPD on MWCNTs and used to form the PBNPs with PB precursors in an acidic solution. The composite maintained good electrical conductivity even wrapped PpPD on MWCNTs and formed stable PBNPs to the strong electrical catalyst for H2O2. This system displayed suitable linear range, low detection limit, and high sensitivity. In addition, it showed high selectivity to ignore interfering species such as ascorbic acid, uric acid, and L-cysteine.

EXPERIMENTAL

Chemicals and Reagents

Multi-walled carbon nanotubes (MWCNTs, 95% purity, diameter 10–15 nm) were purchased from ILJIN Nanotech Co. (Korea). L-cysteine was purchased from Yakuri Pure Chemical Co. (Japan). Ammonium persulfate ((NH4)2S2O8, APS, ≥98.0%), p-phenylenediamine (C6H8N2, pPD, ≥99.0%), potassium ferricyanide (K3Fe(CN)6, 99+%), potassium ferrocyanide (K4Fe(CN)6, 99%), iron(III) chloride (FeCl3, 97%), potassium chloride (KCl), acetic acid (99.7 wt% in H2O), hydrogen peroxide (H2O2, 30 wt% in H2O), Nafion (~5% in a mixture of lower aliphatic alcohols and water), ascorbic acid, uric acid, and glucose were purchased from Sigma-Aldrich Co. (USA). All the chemicals were used without further purification. All the solutions were prepared with deionized water (DIW) obtained from an ultra-pure water purification system (Human Co., Korea) with a resistivity of not less than 18.2 MΩ cm. All the measurements were carried out at room temperature.

Apparatus and Measurements

The surface morphology of composites was characterized by field emission transmission electron microscopy (FE-TEM, JEOL JEM-F200, Japan). TEM samples were prepared by placing a drop of the dispersion on a carbon-coated copper grid. Fourier transform infrared spectra (FT-IR) analyses were performed using a Perkin-Elmer Spectrum 2000 FT-IR spectrometer in the range from 400 to 4000 cm-1 with a KBr pellet. Powder X-ray diffraction (XRD) analyses were performed using a New D8 advance diffractometer. Electrochemical impedance spectroscopy (EIS) measurements were performed using a CHI 760B electrochemical analyzer (CH Instrument, Inc., USA), and cyclic voltammetry (CV) and amperometry were performed using a CHI 842B electrochemical analyzer (CH Instrument, Inc., USA) with the conventional three-electrode system. A bare or modified glassy carbon electrode (GCE, d = 3.0 mm) was used as the working electrode. A platinum (Pt) electrode served as the auxiliary electrode, and a silver/silver chloride (Ag/AgCl) electrode filled with 3 M KCl served as the reference electrode. All ultrasonic cleaning was performed using a US-2510 Ultrasonic Cleaner (Branson, USA). Before each electrochemical measurement, solutions were thoroughly deoxygenated by bubbling N2 gas through the solution for at least 10 min to remove the dissolved oxygen.

Preparation of MWCNTs@PpPD Nanocomposites

2.0 mg of Pristine MWCNTs were added to 17.6 mL of DIW and well dispersed by a 30 min sonication. To this solution, 2.0 mL of 0.1 M pPD and 0.4 mL of 0.1 M APS were added at room temperature under vigorous magnetic stirring for 24 hours. The dark purple solution was carefully filtered through a membrane filter and washed with DIW. The collected precipitate was dried overnight in an oven at 40 ℃. Finally, the product was dispersed in an acidic solution at pH 3.5.

Preparation of MWCNTs@PpPD-PB Nanocomposites

1 mM of K3Fe(CN)6 was added to 25 mL of 0.1 M KCl solution and adjusted to pH 2.7. To this solution, 0.8 mL of dispersed MWCNTs@PpPD solution was added under magnetic stirring for 5 min. 25 mL of 1 mM FeCl3 was added slowly to this mixture with continuous stirring at room temperature for 1 hour. Subsequently, 2 μL of 30% H2O2 was added to the mixed solution. The color of this solution gradually changed from yellowish green to dark blue. After 3 hours, the obtained dispersion was filtered, rinsed with DIW several times, and finally redispersed in 10 mL of DIW.

Electrode Modification

Before modification, the glassy carbon electrode was polished to acquire a mirror-like surface with fine alumina powders (particle size: 0.3 μm and 0.05 μm) on a polishing pad. The electrode was cleaned and sonicated with DIW for 2 min, in sequence. After drying under a gentle stream of N2 gas, the modified electrode was prepared by dropping 8 μL of the MWCNTs@PpPD-PB dispersion onto the purified GCE surface and dried in an oven at 40 ℃ for 2 hours. Then, 2 μL of Nafion (0.5%) was placed on the surface of the modified electrode, and it was dried again in an oven at 40 ℃ for 1 hour.

RESULTS AND DISCUSSION

Characterization of the MWCNTs@PpPD and MWCNTs@PpPD-PB Nanocomposites

Fig. 1 shows the synthetic route for MWCMT@PpPD-PB. At first, pristine MWCNTs were dispersed in a neutral solution through sonication. pPD monomers were added to the solution, and APS was further added resulting in a dark-violet solution. And then, the solution containing MWCNTs@PpPD nanocomposite was added to an acidic solution containing K3Fe(CN)6, and FeCl3 was dripped very slowly. The snapshots of the products for each step are shown in Fig. S1.

JCGMDC_2023_v67n5_339_f0001.png 이미지

Figure 1. Synthesis of MWCNTs@PpPD-PB nanocomposites.

Fig. 2 shows the XRD patterns and FE-TEM images of MWCNTs, MWCNTs@PpPD, and MWCNT@PpPD which suggests the morphologies and sizes of the as-synthesized nanocomposites. The peaks centered at 2θ values of 26.0° and 43.0° correspond to the (002) and (100) reflection planes of graphite from the MWCNTs, respectively.38 The diffraction pattern of the MWCNTs@PpPD nanofibers shows five big sharp peaks (marked as asterisk marks) and eight minor peaks between 2θ = 8-30°.39

JCGMDC_2023_v67n5_339_f0003.png 이미지

Figure 2. XRD pattern (a) of MWCNTs and TEM images (b-c) of MWCNTs at different magnifications, XRD pattern (d) of MWCNTs@PpPD and TEM images (e-f) of MWCNTs@PpPD at different magnifications, XRD pattern (g) of MWCNTs@PpPD-PB and TEM images (h-i) MWCNTs@PpPD-PB at different magnifications.

Compared to the XRD pattern of the MWCNTs@PpPD, the peaks of MWCNTs@PpPD-PB are observed at 2θ values of 17.5°, 24.8°, 35.3°, 39.6° and 43.7° correspond to the (200), (220), (400), (420), and (422) reflection planes, respectively.40 All the reflections can be indexed as a pure face-centered-cubic phase of Fe4[Fe(CN)6]3, which demonstrates the successful synthesis of PB on the modified MWCNTs. Furthermore, the average diameter of the PBNPs was ~15.4 nm, calculated by the Scherrer formula.41 It is analogous to the diameter of the PBNPs of MWCNTs@PpPD nanocomposite determined from the TEM images, which were measured at 10.2-17.5 nm.

Fig. S2 shows the FT-IR spectra of MWCNTs, MWCNTs@PpPD, and MWCNTs@PpPD-PB which investigate the chemical components of the as-synthesized samples. After the polymerization of pPD on the surface of MWCNTs, the characteristic absorption peaks of PpPD were observed. The broad absorption peaks located at 3444 cm-1 and the shoulder peak located at 3216 cm-1 could be because of the N–H stretching vibration of the –NH group in MWCNTs@PpPD. Furthermore, in another broad adsorption peak assigned approximately from 1650 to 1300 cm-1, the two strong peaks located at 1606 and 1545 cm-1 can be attributed to the C=N and C=C stretching vibration of the phenazine ring structure, respectively.42 The other peak located at 1494 cm-1 corresponds to the stretching of the benzene ring. The two small peaks at 1262 and 1208 cm-1 are assigned to the imine C=N stretching vibration of the benzoid and quinoid imine units (–C=N–)42, proving that PpPD was successfully polymerized on MWCNTs. Compared to the MWCNTs@PpPD, the MWCNTs@PpPD-PB nanocomposite showed a strong absorption peak at 2083 cm-1 which is attributed to the C≡N stretching absorption band in the [Fe2+–CN–Fe3+] structure of PB. An absorption peak at 502 cm-1 was also observed, because of the formation of [Fe2+–CN–Fe3+] structure.

Electrochemical Impedance Spectroscopy (EIS) Characterization

The inset in Fig. 3 is the Randles equivalent circuit model used for fitting the measurement data, where Rs for electrolyte resistance, Cdl for double-layer capacitance, Rct for electron transfer resistance, and Zw for Warburg impedance. Rct represents the electron-transfer-limited process at the electrode/electrolyte interface and is equal to the semicircle diameter in the Nyquist diagrams. It is measured in a background solution, containing 5 mM [Fe(CN)6]3- and 5 mM [Fe(CN)6]4- in 0.1 M KCl at the frequency range from 10 mHz to 100 kHz at 0.19 V (vs. Ag/AgCl).

JCGMDC_2023_v67n5_339_f0004.png 이미지

Figure 3. Nyquist plots of (a) bare GCE, (b) GCE/PB, (c) GCE/MWCNTs, (d) GCE/MWCNTs@PpPD and (e) GCE/MWCNTs@PpPD-PB in 0.1 M KCl solution containing 5 mM [Fe(CN)6]3- and 5 mM [Fe(CN)6]4-.

Fig. 3 shows the impedance spectra represented as the Nyquist plots (Z′ vs Z") for (a) bare GCE, (b) GCE/PB, (c) GCE/MWCNTs, (d) GCE/MWCNTs@PpPD, and (e) GCE/MWCNTs@PpPD-PB. Rct of the bare GCE was 127.14 Ω with an almost straight tail line. When MWCNTs were cast on the GCE, Rct decreased to 42.66 Ω, indicating that the MWCNTs could prompt the charge transfer. Furthermore, when the GCE electrode surface was coated with PBNPs, Rct value increased considerably to 421.79 Ω, indicating that the PBNPs on the GCE obstructed the charge transfer of the electrochemical process at the electrode surface. After the MWCNTs@PpPD was dropped over the GCE, the diameter of the high-frequency semicircle was significantly reduced to 9.06 Ω as a Rct value. Rct value of MWCNTs@PpPD-PB was 42.43 Ω, which is greater than that of the MWCNTs@PpPD, indicating that PB interrupted the charge transfer. Note that GCE/MWCNTs@PpPD-PB showed a steeper Warburg slope at low-frequency limit compared to others. This is due to the increased significance of chargetransfer resistance and double-layer capacitance as the frequency rises.43

Voltammetric Behaviors of H2O2 at GCE/MWCNTs@PpPD-PB

Fig. 4(a) shows the CVs of the bare GCE, GCE/MWCNTs, GCE/MWCNTs@PpPD GCE/MWCNTs-PB, and GCE/MWCNTs@PpPD-PB in the absence of H2O2. The redox peaks were observed in the CVs of MWCNTs-PB and MWCNTs@PpPD-PB modified GCE, whereas no redox pair peaks were observed for the bare GCE, GCE/MWCNTs and GCE/MWCNTs@PpPD. Between the observed redox peaks, the peak current of MWCNTs@PpPD-PB modified GCE was much larger than that of MWCNTs-PB modified GCE, probably because of the high electrical conductivity of the coated pPD polymer on the MWCNTs. After adding 5 mM H2O2, the CVs of the bare GCE, GCE/MWCNTs, and GCE/MWCNTs@PpPD did not change (Fig. 4(b)). Meanwhile, the CVs of the MWCNTs-PB and MWCNTs @PpPD-PB modified GCE show that the reduction peak significantly increased, but the oxidation peak decreased slightly. These results indicate the presence of PBNPs on the modified GCE.

JCGMDC_2023_v67n5_339_f0005.png 이미지

Figure 4. CVs of (A) bare GCE, (B) GCE/MWCNTs, (C) GCE/MWCNTs@PpPD, (D) GCE/MWCNTs-PB, and (E) GCE/MWCNTs@PpPD-PB in the (a) absence of H2O2 and (b) present of H2O2 from -0.3 to 0.6 V in 0.1 M KCl solution (pH 2.7) at scan rate of 50 mV/s.

Fig. 5 shows the CVs of GCE/MWCNTs-PB and GCE/MWCNTs@PpPD-PB in N2-saturated 0.1 M KCl (pH 2.7) at different concentrations of H2O2. The cathodic peak current value increases with the increasing concentration of H2O2 because of the PB. Moreover, Fig. S3 shows that the reduction current of the MWCNTs@PpPD-PB-modified GCE is about twice as high as that of the MWCNTs-PB-modified GCE.

JCGMDC_2023_v67n5_339_f0006.png 이미지

Figure 5. CVs of (a) GCE/MWCNTs-PB and (b) GCE/MWCNTs@PpPD-PB in N2-saturated 0.1M KCl aqueous solution (pH 2.7) in the presence of H2O2 with different concentrations (from (A) to (E) : 1, 2, 3, 4, and 5 mM) at a scan rate of 50 mV/s.

Fig. 6 shows the correlation between the current and the scan rate. The electrochemical effect of the scan rate on the electrocatalytic H2O2 cathodic current was investigated to evaluate the kinetics of the electrochemical performance of the as-synthesized MWCNTs nanocomposites. The cathodic peak currents increased linearly in proportion to the square root of the scan rate with a correlation coefficient of 0.9978 as the scan rate was increased from 10 to 200 mV/s (Fig. S4). This result signifies that the process of electrocatalytic reaction is diffusion-controlled.

JCGMDC_2023_v67n5_339_f0007.png 이미지

Figure 6. CVs of MWCNTs@PpPD-PB nanocomposite modified glassy carbon electrode in N2-saturated 0.1M KCl aqueous solution (pH 2.7) in the presence of 1 mM H2O2 at different scan rates (from (a) to (e) : 10, 20, 50, 100, 200 mV/s).

Amperometric Response of H2O2 at GCE/MWCNTs-PB and GCE/MWCNTs@PpPD-PB

Fig. 7 shows the comparative amperometric response of GCE/MWCNTs-PB and GCE/MWCNTs@PpPD-PB for successive sensing of H2O2 into stirred N2-saturated 0.1 M KCl (pH 2.7) aqueous solution at an applied potential of +0.15 V (vs. Ag/AgCl). The reduction current value showed a rapid response to the subsequent addition of H2O2 and quickly reached the 95% steady-state current, which was less than 2 sec. In the MWCNTs-PB modified GCE and the MWCNTs@PpPD-PB modified GCE, the current for both increased with the addition of H2O2, and cathodic current of the latter was greater than the former.

JCGMDC_2023_v67n5_339_f0008.png 이미지

Figure 7. Amperometric response of the (a) MWCNTs-PB and (b) MWCNTs@PpPD-PB nanocomposite modified glassy carbon electrode to successive injection of H2O2 in 0.1 M KCl aqueous solution (pH 2.7) under stirring. Applied potential: +0.15 V.

The amperometric response was unstable with the addition of H2O2 probably because of the weak interaction between the MWCNTs and the PBNPs. To solve this problem, the MWCNTs were effectively coated by PpPD and strongly attached to the PBNPs. Therefore, the modified electrode composed of the MWCNTs, PpPD, and PB showed a better response to the addition of H2O2.

Fig. S5 shows the calibration curve of the reduction peak currents versus the concentration of H2O2, which presents the linear response region of MWCNTs-PB and MWCNTs@PpPD-PB. MWCNTs@PpPD-PB shows a linear response range from 0.005 mM to 2.225 mM with a correlation coefficient of 0.9992, and the sensitivity of the sensor was 583.6 μAmM-1cm-2, which was much higher than that of a previous study estimated from GCE/MWCNTs-PB and GCE/MWCNTs/Ppy/PB.44-45 Moreover, the limit of detection of GCE/MWCNTs@PpPD-PB was calculated as 0.95 μM with a signal-to-noise ratio of 3.

Table 1 summarizes the electrochemical characteristics of various modified electrodes containing PB for sensing H2O2, indicating that GCE/MWCNTs@PpPD-PB has a high sensitivity and a wide linear range in comparison to those of other reported modified electrodes, proving improved electrical conductivity and good stability.

Table 1. Electrochemical characteristics of various PB contained electrodes toward hydrogen peroxide

JCGMDC_2023_v67n5_339_t0001.png 이미지

Selectivity, Reproducibility, and Stability of the Sensor

An amperometric measurement was performed to assess the selectivity of the as-synthesized sensor. Fig. 8 shows that the current generated in the presence of 0.1 mM of interfering substances, such as citric acid, ascorbic acid, and L-cysteine. These interfering substances could be ignored relative to the current attributed solely to 0.1 mM H2O2 in N2-saturated 0.1 M KCl solution (pH 2.7) at an applied potential of 0.15 V, indicating that this sensor possessed an excellent anti-interference capacity.

JCGMDC_2023_v67n5_339_f0009.png 이미지

Figure 8. Interference-free behavior of the MWCNTs@PpPD-PB nanocomposite modified GCE by the amperometric trace recorded in N2-saturated 0.1 M KCl solution (pH 2.7) under stirring. Injection of 0.1 mM H2O2, citric acid (CA), ascorbic acid (AA), and L-Cysteine (L-Cys). Applied potential: +0.15 V.

The reproducibility was investigated by the CV experiments on five modified electrodes under the same condition, showing excellent performance as evidenced by a relative standard deviation (R.S.D) of 2.5%. Fig. S6 shows the stability examined by monitoring the current response in 1 mM H2O2 solution after effectively cycling the modified electrode. After 50 cycles, the reduction of the electrode signal was only 2.6%, and after 50 additional cycles, the loss of signal reached only 2.9%. To determine the life span of the prepared sensor, the as-synthesized electrodes were stored in a refrigerator at 4 ℃, and the electrode signal decreased to only 97.1% of its original peak current in 1 mM H2O2 after 20 days. In brief, MWCNTs@PpPD-PB on the GCE showed excellent selectivity, reproducibility, and stability. The current response becomes stable in less than 2 sec, which indicates the rapid response of the MWCNTs@PpPD-PB on the GCE towards H2O2.

The performance of MWCNTs@PpPD-PB nanocomposites on the GCE was analyzed by applying it to the determination of H2O2 present in the household disinfectant. Using amperometry measurement to obtain the response of the household disinfectant, the experiment was conducted under the same conditions as the conventional amperometry experimental conditions. The exact H2O2 concentration in the household disinfectant was measured using the standard addition method.

The concentrations obtained by stepwise increasing the concentration and the R.S.D are listed in Table 2. The recoveries of H2O2 were found to be in the range of 95-100%, indicating that the modified electrode is suitable for real sample analyses.

Table 2. Electroanalytical values obtained from the determination of H2O2 in household disinfectant using i-t curve in 0.1 M KCl solution (pH 2.7) by MWCNTs@PpPD-PB composites modified GCE

JCGMDC_2023_v67n5_339_t0002.png 이미지

aR.S.D. (%) calculated from five separate experiments

CONCLUSION

In this study, an approach for simple fabrication of sensitive electrochemical detection of H2O2 was described based on MWCNTs@PpPD-PB nanocomposites. The MWCNTs@PpPD was easily synthesized without structural destruction of MWCNTs by PpPD coating. PpPD not only serves to ensure good dispersion of MWCNTs in the water but also increased the reduction currents of H2O2. This modified electrode showed a good electrocatalytic response for reduction of H2O2 in an acidic solution and showed good linear range, high sensitivity, low detection limit, good reproducibility, and better stability. Finally, MWCNTs@PpPD-PB modified glassy carbon electrode was applied successfully to detect H2O2 in real samples as a sensor.

Acknowledgments

Publication cost of this paper was supported by the Korean Chemical Society.

참고문헌

  1. Baghayeri, M.; Zare, E. N.; Lakouraj, M. M. Biosens. Bioelectron. 2014, 55, 259. 
  2. Xue, Y.; Wang, Y.; Pan, Z.; Sayama, K. Angew. Chem. Int. Ed. 2021, 60, 10469. 
  3. Ye, S.; Hananya, N.; Green, O.; Chen, H.; Zhao, A. Q.; Shen, J.; Shabat, D.; Yang, D. Angew. Chem. Int. Ed. 2020, 59, 14326. 
  4. Deng, W.; Peng, Y.; Yang, H.; Tan, Y.; Ma, M.; Xie, Q.; Chen, S. ACS Appl. Mater. Interfaces 2019, 11, 29072. 
  5. Zou, J.; Cai, H.; Wang, D.; Xiao, J.; Zhou, Z.; Yuan, B. Chemosphere 2019, 224, 646.  https://doi.org/10.1016/j.chemosphere.2019.03.005
  6. Chen, A.; Chatterjee, S. Chem. Soc. Rev. 2013, 42, 5425. 
  7. Yi, H.; Qin, R.; Ding, S.; Wang, Y.; Li, S.; Zhao, Q.; Pan, F. Adv. Funct. Mater. 2020, 31, 2006970.  https://doi.org/10.1002/adfm.202006970
  8. Ricci, F.; Palleschi, G. Biosens. Bioelectron. 2005, 21, 389. 
  9. Nai, J.; Lou, X. W. D. Adv. Mater. 2019, 31, e1706825.  https://doi.org/10.1002/adma.201706825
  10. Komkova, M. A.; Pasquarelli, A.; Andreev, E. A.; Galushin, A. A.; Karyakin, A. A. Electrochim. Acta 2020, 339, 135924.  https://doi.org/10.1016/j.electacta.2020.135924
  11. Aller-Pellitero, M.; Fremeau, J.; Villa, R.; Guirado, G.; Lakard, B.; Hihn, J.; del Campo, F. J. Sens. Actuators B Chem. 2019, 290, 591. 
  12. Chaudhary, A.; Pathak, D. K.; Ghosh, T.; Kandpal, S.; Tanwar, M.; Rani, C.; Kumar, R. ACS Appl. Electron. Mater. 2020, 2, 1768.  https://doi.org/10.1021/acsaelm.0c00342
  13. Weidinger, D.; Brown, D. J.; Owrutsky, J. C. J. Chem. Phys. 2011, 134, 124510. 
  14. Zhou, P.; Xue, D.; Luo, H.; Chen, X. Nano Lett. 2002, 2, 845. 
  15. Huang, Y.; Ren, S. Appl. Mater. Today 2021, 22, 100886. 
  16. Matos-Peralta, Y.; Antuch, M. J. Electrochem. Soc. 2019, 167, 037510. 
  17. Ying, S.; Chen, C.; Wang, J.; Lu, C.; Liu, T.; Kong, Y.; Yi, F. Y. ChemPlusChem 2021, 86, 1608. 
  18. Nossol, E.; Zarbin, A. J. Adv. Func. Mater. 2009, 19, 3980. 
  19. Cao, L.; Liu, Y.; Zhang, B.; Lu, L. ACS Appl. Mater. Interfaces 2010, 2, 2339. 
  20. Sitnikova, N. A.; Komkova, M. A.; Khomyakova, I. V.; Karyakina, E. E.; Karyakin, A. A. Anal. Chem. 2014, 86, 4131. 
  21. Neff, V. D. J. Electrochem. Soc. 1978, 125, 886. 
  22. Zeng, J.; Wei, W.; Liu, X.; Wang, Y.; Luo, G. Microchim. Acta 2007, 160, 261. 
  23. Haghighi, B.; Hamidi, H.; Gorton, L. Sens. Actuators B Chem. 2010, 147, 270. 
  24. Miao, Y.; Liu, J. Sci. Technol. Adv. Mater. 2009, 10, 025001. 
  25. Zhang, L.; Song, Z.; Zhang, Q.; Jia, X.; Zhang, H.; Xin, S. Electroanalysis 2009, 21, 1835.  https://doi.org/10.1002/elan.200904620
  26. Zhu, X.; Niu, X.; Zhao, H.; Lan, M. Sens. Actuators B Chem. 2014, 195, 274. 
  27. Cabrera-Garcia, A.; Vidal-Moya, A.; Bernabeu, A.; Sanchez-Gonzalez, J.; Fernandez, E.; Botella, P. Dalton Trans. 2015, 44, 14034. 
  28. Guari, Y.; Larionova, J.; Molvinger, K.; Folch, B.; Guerin, C. Chem. Commun. 2006, 24, 2613. 
  29. Uemura, T.; Kitagawa, S. J. Am. Chem. Soc. 2003, 125, 7814. 
  30. Uemura, T.; Ohba, M.; Kitagawa, S. Inorg. Chem. 2004, 43, 7339.  https://doi.org/10.1021/ic0488435
  31. McHale, R.; Ghasdian, N.; Liu, Y.; Ward, M. B.; Hondow, N. S.; Wang, H.; Miao, Y.; Brydson, R.; Wang, X. Chem. Commun. 2010, 46, 4574. 
  32. Kim, F. S.; Ren, G.; Jenekhe, S. A. Chem. Mater. 2011, 23, 682. 
  33. Haldorai, Y.; Lyoo, W. S.; Shim, J. J. Colloid Polym. Sci. 2009, 287, 1273. 
  34. Han, X.; Liu, S. J.; Yuan, Y.; Wang, Y.; Hu, L. J. J. Alloys Compd. 2012, 543, 200. 
  35. Haldorai, Y.; Long, P. Q.; Noh, S. K.; Lyoo, W. S.; Shim, J. J. Polym. Adv. Technol. 2011, 22, 781. 
  36. Huang, W.; Zhou, X.; Luan, Y.; Cao, Y.; Wang, N.; Lu, Y.; Liu, T.; Xu, W. J. Sep. Sci. 2020, 43, 954. 
  37. Li, C.; Wang, Y.; Li, H.; Liu, J.; Song, J.; Fusaro, L.; Hu, Z. Y.; Chen, Y.; Li, Y.; Su, B. L. J. Energy Chem. 2021, 59, 396. 
  38. Jiang, Y.; Lu, Y.; Zhang, L.; Liu, L.; Dai, Y.; Wang, W. J. Nanoparticle Res. 2012, 14, 938. 
  39. Yang, S.; Liao, F. Synth. Met. 2012, 162, 1343. 
  40. Wu, X.; Cao, M.; Hu, C.; He, X. Cryst. Growth Des. 2006, 6, 26. 
  41. Patterson, A. Phys. Rev. 1939, 56, 978. 
  42. Li, X. G.; Huang, M. R.; Duan, W.; Yang, Y. L. Chem. Rev. 2002, 102, 2925.  https://doi.org/10.1021/cr010423z
  43. Bard, A. J.; Faulkner, L. R. Electrochemical Methods: Fundamentals and Applications, 2nd Ed.; Wiley: New York, U. S. A., 2000; pp. 384-385. 
  44. Zhai, J.; Zhai, Y.; Wen, D.; Dong, S. Electroanalysis 2009, 21, 2207.  https://doi.org/10.1002/elan.200904680
  45. Jin, E.; Bian, X.; Lu, X.; Wang, C. J. Mater. Sci. 2012, 47, 4326. 
  46. Zou, Y.; Sun, L.; Xu, F. Talanta 2007, 72, 437. 
  47. Zhang, L.; Song, Z.; Zhang, Q.; Jia, X.; Zhang, H.; Xin, S. Electroanalysis 2009, 21, 1835.  https://doi.org/10.1002/elan.200904620
  48. Jin, E.; Lu, X.; Cui, L.; Chao, D.; Wang, C. Electrochim. Acta 2010, 55, 7230. 
  49. Zhang, J.; Li, J.; Yang, F.; Zhang, B.; Yang, X. Sens. Actuators B Chem. 2009, 143, 373. 
  50. Silva, S. C.; Cardoso, R. M.; Richter, E. M.; Munoz, R. A. A.; Nossol, E. Mater. Chem. Phys. 2020, 250, 123011.