The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Rapid and Sensitive Detection of the Causal Agents of Postharvest Kiwifruit Rot, Botryosphaeria dothidea and Diaporthe eres, Using a Recombinase Polymerase Amplification Assay

  • Gi-Gyeong Park (Department of Applied Biology, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Wonyong Kim (Korean Lichen Research Institute, Sunchon National University) ;
  • Kwang-Yeol Yang (Department of Applied Biology, College of Agriculture and Life Sciences, Chonnam National University)
  • Received : 2023.07.06
  • Accepted : 2023.08.10
  • Published : 2023.10.01
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Abstract

The occurrence of postharvest kiwifruit rot has caused great economic losses in major kiwifruit-producing countries. Several pathogens are involved in kiwifruit rot, notably Botryosphaeria dothidea, and Diaporthe species. In this study, a recombinase polymerase amplification (RPA) assay was developed for the rapid and sensitive detection of the pathogens responsible for posing significant threats to the kiwifruit industries. The RPA primer pairs tested in this study were highly specific for detection of B. dothidea and D. eres. The detection limits of our RPA assays were approximately two picograms of fungal genomic DNA. The optimal conditions for the RPA assays were determined to be at a temperature of 39℃ maintained for a minimum duration of 5 min. We were able to detect the pathogens from kiwifruit samples inoculated with a very small number of conidia. The RPA assays enabled specific, sensitive, and rapid detection of B. dothidea and D. eres, the primary pathogens responsible for kiwifruit rots in South Korea.

Keywords

Acknowledgement

This work was supported by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET), funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) (Nos. 618001-05-5-SB120 and 321001-03-3-SB070).

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