Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
권호 표지
DOI QR코드

DOI QR Code

A case of hepatic anisakidosis caused by Anisakis pegreffii mimicking liver cancer

  • Minoru Yamada (Department of Infectious Diseases, Kyoto Prefectural University of Medicine) ;
  • Fumi Murakoshi (Department of Infectious Diseases, Kyoto Prefectural University of Medicine) ;
  • Hisashi Ikoma (Department of Gastroenterological Surgery, Kyoto Prefectural University of Medicine) ;
  • Osamu Inamori (Department of Surgical Pathology, Kyoto Prefectural University of Medicine) ;
  • Akio Yanagisawa (Department of Surgical Pathology, Kyoto Prefectural University of Medicine) ;
  • Eiichi Konishi (Department of Surgical Pathology, Kyoto Prefectural University of Medicine)
  • Received : 2023.05.09
  • Accepted : 2023.06.25
  • Published : 2023.08.31
Download PDF
⟨ Previous Next ⟩

Abstract

Extra-gastrointestinal anisakidosis is rare. We herein report an Anisakis pegreffii infection in a patient with hepatic anisakidosis diagnosed based on its molecular identification. A 71-year-old male patient had a hepatic tumor presenting as a low-density area of 20 mm in diameter in segment 6 of the liver on abdominal ultrasonography, computed tomography, and magnetic resonance imaging. The surgically resected pathological specimen revealed a necrotizing eosinophilic granuloma containing nematode larvae, possibly an Anisakis larva. Molecular and phylogenetic analysis demonstrated Anisakis larvae belonging to A. pegreffii. The present results will help identify and characterize unknown Anisakis species in histological sections.

Keywords

Acknowledgement

This study was performed in accordance with the ethical standards of the Institutional Review Board of the Kyoto Prefectural University of Medicine and with the Declaration of Helsinki as revised in 2013 and an informed consent for publication was obtained from the patient.

References

  1. Chai JY, Murrell KD, Lambery AJ. Fish-borne parasitic zoonoses: status and issues. Int J Parasitol 2005;35(11-12):1233-1254. https://doi.org/10.1016/j.ijpara.2005.07.013 
  2. Sohn WM, Chai JY. Anisakiosis (anisakidosis). In Palmer SR, Soulsby L, Torgerson PR, Brown WG, eds. Oxford Textbook of Zoonoses. 2nd ed. Oxford University Press. Oxford, UK. 2011, pp 774-786. 
  3. Ishikura H. 5. Anisakiasis. (2) Clinical pathology and epidemiology. In Otsuru M, Kamegai S, Hayashi S. eds, Progress of Medical Parasitology in Japan. vol.8. Meguro Parasitological Museum, Tokyo, Japan. 2003, pp 451-473. 
  4. Takahashi S, Ishikura H, Kikuchi K. Anisakidosis: global point of view. In Ishikura H ed, Host Response to International Parasitic Zoonoses. Springer-Verlag, New York, USA. 1998, pp 109-120. 
  5. Takabe K, Ohki S, Kunihiro O, Sakashita T, Endo I, et al. Anisakidosis: a cause of intestinal obstruction from eating sushi. Am J Gastroenterol 1998;93(7):1172-1173. https://doi.org/10.1111/j.1572-0241.1998.00356.x 
  6. Arizono N, Miura T, Yamada M, Tegoshi T, Onishi K. Human infection with Pseudoterranova azarasi roundworm. Emeg Infect Dis 2011;17(3):555-556. https://doi.org/10.3201/eid1703.101350 
  7. Mitsuboshi A, Yamaguchi H, Ito Y, Mizuno T, Tokoro M, et al. Extra-gastrointestinal anisakidosis caused by Pseudoterranova azarasi manifesting as strangulated inguinal hernia. Parasitol Int 2017;66(6):810-812. https://doi.org/10.1016/j.parint.2017.09.008 
  8. Mattiucci S, Nascetti G. Advances and trends in the molecular systematics of anisakid nematodes, with implications for their evolutionary ecology and host-parasite co-evolutionary processes. Adv Parasitol 2008;66:47-148. https://doi.org/10.1016/S0065-308X(08)00202-9 
  9. Umehara A, Kawakami Y, Araki J, Uchida A. Molecular identification of the etiological agent of the human anisakiasis in Japan. Parasitol Int 2007;56(3):211-215. https://doi.org/10.1016/j.parint.2007.02.005 
  10. Valentini A, Matticci S, Bondanelli P, Webb SC, Mignucci-Giannone AA, et al. Genetic relationships among Anisakis species (Nematoda: Anisakidae) inferred from mitochondrial cox2 sequences and comparison with allozyme data. J Parasitol 2006;92(1):156-166. https://doi.org/10.1645/GE-3504.1 
  11. Larkin MA, Blackshields G, Brown NP, Chenna R, McGettingen PA, et al. Clustal W and Clustal X version 2.0. Bioinformatics 2007;23(21):2947-2948. https://doi.org/10.1093/bioinformatics/btm404 
  12. Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 1999;41:95-98. 
  13. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis Version 6.0. Mol Biol Evol 2013;30(12):2725-2729. https://doi.org/10.1093/molbev/mst197 
  14. Arizono N, Yamada M, Tegoshi T, Yoshikawa M. Anisakis simplex sense stricto and Anisakis pegreffii: biological characteristics and pathogenic potential in human anisakiasis. Foodborne Pathog Dis 2012;9(6):517-521. https://doi.org/10.1089/fpd.2011.1076 
  15. Jeon CH, Kim JH. Pathogenic potential of the two sibling species, Anisakis simplex (s.s.) and Anisakis pegreffii (Nematoda: Anisakidae): in vitro and in vivo studies. Biomed Res Int 2015;2015:983656. https://doi.org/10.1155/2015/983656. 
  16. Mattiucci S, Paoletti M, Borrini F, Palumbo M, Palmieri RM, et al. First molecular identification of the zoonotic parasite Anisakis pegreffi (Nematoda: Anisakidae) in a paraffin-embedded granuloma taken from a case of human intestinal anisakiasis in Italy. BMC Infect Dis 2011;11:82. https://doi.org/10.1186/1471-2334-11-82 
  17. Mladineo I, Popovic M, Drmic-Hofman I, Poljak V. A case report of Anisakis pegreffii (Nematoda, Anisakidae) identified from archival paraffin sections of a Croatian patient. BMC Infect Dis 2016;16:42. https://doi.org/10.1186/s12879-016-1401-x 
  18. Kagei N, Orikasa H, Hori E, Sannomiya A, Yasumura Y. A case of hepatic anisakiasis with a literal survey for extra-gastrointestinal anisakiasis. Jpn J Parasitol 1995;44:346-351. 
  19. Murata Y, Ando K, Usui M, Sugiyama H, Hayashi A, et al. A case of hepatic anisakiasis caused by Pseudoterranova decipiens mimicking metastatic liver cancer. BMC Infect Dis 2018;18(1):619. https://doi.org/10.1186/s12879-018-3540-8