Journal of Acupuncture Research

  • 제40권2호
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  • Pages.135-142
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  • 2023
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  • 2586-288X(pISSN)
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  • 2586-2898(eISSN)
대한침구의학회 (Korean Acupuncture & Moxibustion Medicine Society)
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Effects of Scutellaria baicalensis Extract on Gout-Induced Rats

  • Eunchang Lee (Department of Acupuncture & Moxibustion Medicine, College of Korean Medicine, Gachon University) ;
  • Ho-Sueb Song (Department of Acupuncture & Moxibustion Medicine, College of Korean Medicine, Gachon University)
  • 투고 : 2023.01.04
  • 심사 : 2023.03.22
  • 발행 : 2023.05.31
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초록

Background: This study aimed to investigate hyperuricemia, renal inflammation, and xanthine oxidase (XO) activity improvement in a rat model treated with Scutellaria baicalensis extract (SBE). Methods: The rats were divided into 4 groups (n = 5 each), including sham, potassium oxonate (PO) injected hyperuricemia (control group), PO + 10 mg/kg allopurinol administrated (allopurinol group), and a PO + 50 mg/kg SBE administrated (SBE group), to investigate the effectiveness and molecular mechanisms of SBE. The effects of SBE on PO-induced hyperuricemia rats, renal inflammation, and XO activity were measured. Body weight and organ index of the kidney and liver were measured in PO-induced hyperuricemia rats, and serum uric acid level was extracted from whole blood and was measured. Renal inflammation was observed under a microscope after sections. XO activity was measured by liver tissue and serum XO levels. Results: Organ indexes of the kidney and liver in rats were significantly decreased in the allopurinol group than in the control group and with no significant difference in the SBE group. A PO injection for 5 days significantly increased serum uric acid levels in the control group compared to the sham group. Meanwhile, the SBE and allopurinol groups have significantly decreased serum uric acid levels compared to the control group. The SBE group revealed effectively improved renal histopathological changes compared to the control group. The XO inhibitor, allopurinol, significantly decreased XO activity. Additionally, SBE significantly lowered XO activity in rats. Conclusion: SBE can be used as an effective treatment for gout in the future.

키워드

과제정보

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No.: 2020R1A2C1013956).

참고문헌

  1. Bursill D, Taylor WJ, Terkeltaub R, Kuwabara M, Merriman TR, Grainger R, et al. Gout, Hyperuricemia, and Crystal-Associated Disease Network consensus statement regarding labels and definitions for disease elements in gout. Arthritis Care Res (Hoboken) 2019;71:427-434. doi: 10.1002/acr.23607
  2. Takiue Y, Hosoyamada M, Kimura M, Saito H. The effect of female hormones upon urate transport systems in the mouse kidney. Nucleosides Nucleotides Nucleic Acids 2011;30:113-119. doi: 10.1080/15257770.2010.551645
  3. Hak AE, Choi HK. Menopause, postmenopausal hormone use and serum uric acid levels in US women--the Third National Health and Nutrition Examination Survey. Arthritis Res Ther 2008;10:R116. doi: 10.1186/ar2519
  4. Wallace KL, Riedel AA, Joseph-Ridge N, Wortmann R. Increasing prevalence of gout and hyperuricemia over 10 years among older adults in a managed care population. J Rheumatol 2004;31:1582-1587.
  5. Dalbeth N, Phipps-Green A, Frampton C, Neogi T, Taylor WJ, Merriman TR. Relationship between serum urate concentration and clinically evident incident gout: an individual participant data analysis. Ann Rheum Dis 2018;77:1048-1052. doi: 10.1136/annrheumdis-2017-212288
  6. Ichida K, Matsuo H, Takada T, Nakayama A, Murakami K, Shimizu T, et al. Decreased extra-renal urate excretion is a common cause of hyperuricemia. Nat Commun 2012;3:764. doi: 10.1038/ncomms1756
  7. Chhana A, Lee G, Dalbeth N. Factors influencing the crystallization of monosodium urate: a systematic literature review. BMC Musculoskelet Disord 2015;16:296. doi: 10.1186/s12891-015-0762-4
  8. Terkeltaub R. What makes gouty inflammation so variable? BMC Med 2017;15:158. doi: 10.1186/s12916-017-0922-5
  9. Robinson PC. Gout - an update of aetiology, genetics, co-morbidities and management. Maturitas 2018;118:67-73. doi: 10.1016/j.maturitas.2018.10.012
  10. Terkeltaub R. Update on gout: new therapeutic strategies and options. Nat Rev Rheumatol 2010;6:30-38. doi: 10.1038/nr-rheum.2009.236
  11. Hwang JH, Lee KH, Nam DW, Yook TH, Song HS. Current status of clinical practice for gout in Korean medicine - on-line (web-based) survey. Korean J Acupunct 2021;38:16-31. doi: 10.14406/acu.2021.003
  12. Zhao T, Tang H, Xie L, Zheng Y, Ma Z, Sun Q, et al. Scutellaria baicalensis Georgi. (Lamiaceae): a review of its traditional uses, botany, phytochemistry, pharmacology and toxicology. J Pharm Pharmacol 2019;71:1353-1369. doi: 10.1111/jphp.13129
  13. Cheng CS, Chen J, Tan HY, Wang N, Chen Z, Feng Y. Scutellaria baicalensis and cancer treatment: recent progress and perspectives in biomedical and clinical studies. Am J Chin Med 2018;46:25-54. doi: 10.1142/S0192415X18500027
  14. Zhao Q, Chen XY, Martin C. Scutellaria baicalensis, the golden herb from the garden of Chinese medicinal plants. Sci Bull (Beijing) 2016;61:1391-1398. doi: 10.1007/s11434-016-1136-5
  15. Kim HJ, Bak SR, Ha HJ, Kim YS, Lee BK, An WG. Systemic analysis of antibacterial and pharmacological functions of Scutellariae Radix. J Physiol Pathol Korean Med 2020;34:184-190. doi: 10.15188/kjopp.2020.08.34.4.184
  16. Ahn SH, Kim KB. Effect of anti-inflammation through creation of skin fat barrier on Scutellaria baicalensis extract. J Pediatr Korean Med 2021;35:40-47. doi: 10.7778/jpkm.2021.35.1.40
  17. Kim BH. The effects of Scutellaria Radix extract on the alcohol-induced fatty acid synthesis of liver in rats. J Korean Med Obes Res 2018;18:19-26. doi: 10.15429/jkomor.2018.18.1.19
  18. Choi HK, Atkinson K, Karlson EW, Willett W, Curhan G. Alcohol intake and risk of incident gout in men: a prospective study. Lancet 2004;363:1277-1281. doi: 10.1016/S0140-6736(04)16000-5
  19. Choi HK, Atkinson K, Karlson EW, Willett W, Curhan G. Purine-rich foods, dairy and protein intake, and the risk of gout in men. N Engl J Med 2004;350:1093-1103. doi: 10.1056/NEJMoa035700
  20. Choi HK, Liu S, Curhan G. Intake of purine-rich foods, protein, and dairy products and relationship to serum levels of uric acid: the Third National Health and Nutrition Examination Survey. Arthritis Rheum 2005;52:283-289. doi: 10.1002/art.20761
  21. Qaseem A, Harris RP, Forciea MA; Clinical Guidelines Committee of the American College of Physicians; Denberg TD, Barry MJ, et al. Management of acute and recurrent gout: a clinical practice guideline from the American College of Physicians. Ann Intern Med 2017;166:58-68. doi: 10.7326/M16-0570
  22. Hung SI, Chung WH, Liou LB, Chu CC, Lin M, Huang HP, et al. HLA-B*5801HLA-B*5801 allele as a genetic marker for severe cutaneous adverse reactions caused by allopurinol. Proc Natl Acad Sci U S A 2005;102:4134-4139. doi: 10.1073/pnas.0409500102 Erratum in: Proc Natl Acad Sci U S A 2005;102:6237.
  23. FitzGerald JD, Dalbeth N, Mikuls T, Brignardello-Petersen R, Guyatt G, et al. 2020 American College of Rheumatology guideline for the management of gout. Arthritis Care Res (Hoboken) 2020;72:744-760. doi: 10.1002/acr.24180 Erratum in: Arthritis Care Res (Hoboken) 2020;72:1187. Erratum in: Arthritis Care Res (Hoboken) 2021;73:458.
  24. Zhang SJ, Liu JP, He KQ. Treatment of acute gouty arthritis by blood-letting cupping plus herbal medicine. J Tradit Chin Med 2010;30:18-20. doi: 10.1016/s0254-6272(10)60005-2
  25. Zhang KH, Wang MQ, Wei LL, Feng CJ, Zhang YS, Teng JB. Investigation of the effects and mechanisms of Dendrobium loddigesii Rolfe extract on the treatment of gout. Evid Based Complement Alternat Med 2020;2020:4367347. doi: 10.1155/2020/4367347
  26. Wu XH, Wang CZ, Wang SQ, Mi C, He Y, Zhang J, et al. Anti-hyperuricemia effects of allopurinol are improved by Smilax riparia, a traditional Chinese herbal medicine. J Ethnopharmacol 2015;162:362-368. doi: 10.1016/j.jep.2015.01.012
  27. Wang CC, Li YL, Chiu PY, Chen C, Chen HC, Chen FA. Protective effects of corni fructus extract in mice with potassium oxonate-induced hyperuricemia. J Vet Med Sci 2022;84:1134-1141. doi: 10.1292/jvms.21-0671
  28. Xiao YZ, Ye ZZ, Liang YT, Chen XP, Wang YH, Xu Q, et al. Association between Chinese herbal medicine therapy and the risk of chronic kidney disease in gout patients. Front Pharmacol 2021;12:661282. doi: 10.3389/fphar.2021.661282
  29. Wang J, Chen S, Zhang J, Wu J. Scutellaria baicalensis georgi is a promising candidate for the treatment of autoimmune diseases. Front Pharmacol 2022;13:946030. doi: 10.3389/fphar.2022.946030
  30. Yang X, Yang J, Zou H. Baicalin inhibits IL-17-mediated joint inflammation in murine adjuvant-induced arthritis. Clin Dev Immunol 2013;2013:268065. doi: 10.1155/2013/268065
  31. Bai L, Bai Y, Yang Y, Zhang W, Huang L, Ma R, et al. Baicalin alleviates collagen-induced arthritis and suppresses TLR2/MYD88/NF-κB p65 signaling in rats and HFLS-RAs. Mol Med Rep 2020;22:2833-2841. doi: 10.3892/mmr.2020.11369
  32. Jung SM, Schumacher HR, Kim H, Kim M, Lee SH, Pessler F. Reduction of urate crystal-induced inflammation by root extracts from traditional oriental medicinal plants: elevation of prostaglandin D2 levels. Arthritis Res Ther 2007;9:R64. doi: 10.1186/ar2222