Journal of Species Research

The National Institute of Biological Resources (국립생물자원관)
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A new record of Ardisia×walkeri, a hybrid of A. japonica and A. pusilla, (Primulaceae) from Jeju Island, Korea

  • Goro Kokubugata (Department of Botany, National Museum of Nature and Science) ;
  • Satoshi Kakishima (Center for Molecular Biodiversity Research, National Museum of Nature and Science) ;
  • Chan-ho Park (National Institute of Biological Resources) ;
  • Takuro Ito (The Center for Academic Resources and Archives/Botanical Gardens, Tohoku University) ;
  • Atsushi Abe (Okinawa Churashima Research Center, Okinawa Churashima Foundation) ;
  • Chikako Ishii (Department of Botany, National Museum of Nature and Science) ;
  • Gwan-Pil Song (Jeju Environment Research Institute)
  • Received : 2023.04.23
  • Accepted : 2023.07.11
  • Published : 2023.08.31
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Abstract

We conducted phylogenetic analyses using multiplexed inter-simple sequence repeat genotyping by sequencing and compared chloroplast DNA sequences among Ardisia japonica, A. pusilla, and morphologically intermediate plants found on Jeju Island, Korea. Our network analysis demonstrated that the intermediate plants were genetically positioned between A. japonica and A. pusilla. Our comparison of the intergenic spacer between the psbA and trnH genes in chloroplast DNA indicated that four nucleotide substitutions separate A. japonica and A. pusilla, whereas the intermediate plants exhibited the A. japonica haplotype. Our results suggest that the intermediate plants on Jeju Island represent a natural hybrid of A. japonica, as the maternal species, and A. pusilla, and that they are attributable to Ardisia×walkeri. This record constitutes the first documented occurrence of the hybrid taxon in Korea.

Keywords

Acknowledgement

We are grateful to R. Matsuda for the assistance with the molecular analyses. This study was conducted under the Memorandum of Understanding between the National Museum of Nature and Science, Japan, and the National Institute of Biological Recourses, Korea, on Cooperation in Scientific Research; and was financially supported by the Mitsubishi Cooperation in part.

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