The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Antioxidant Activity and Anti-Obesity Effects of Mixture of Brassica juncea and Black Rice Bran

갓과 흑미강 혼합물의 항산화 활성 및 지방세포 억제 효과

  • Ji-Heon Im (Dept. of Food Biotechnology and Environmental Science, Kangwon National University) ;
  • June seok Lim (Dept. of Food Science and Biotechnology, Kangwon National University) ;
  • Xionggao Han (Dept. of Food Biotechnology and Environmental Science, Kangwon National University) ;
  • Xiao Men (Dept. of Food Biotechnology and Environmental Science, Kangwon National University) ;
  • Geon Oh (Dept. of Food Biotechnology and Environmental Science, Kangwon National University) ;
  • Geun hee Cho (Dept. of Food Science and Biotechnology, Kangwon National University) ;
  • Sun-Il Choi (Agricultural and Life Sciences Research Institute, Kangwon National University) ;
  • Woonsang Hwang (STR Biotech Co., Ltd.) ;
  • Ok-Hwan Lee (Dept. of Food Biotechnology and Environmental Science, Kangwon National University)
  • 임지현 (강원대학교 식품환경융합학과 ) ;
  • 임준석 (강원대학교 바이오산업공학부 식품생명공학전공 ) ;
  • 한웅호 (강원대학교 식품환경융합학과 ) ;
  • 문효 (강원대학교 식품환경융합학과 ) ;
  • 오건 (강원대학교 식품환경융합학과 ) ;
  • 조근희 (강원대학교 바이오산업공학부 식품생명공학전공 ) ;
  • 최선일 (강원대학교 농업생명과학연구원) ;
  • 황운상 ((주)에스티알 바이오텍 ) ;
  • 이옥환 (강원대학교 식품환경융합학과)
  • Received : 2023.07.10
  • Accepted : 2023.07.28
  • Published : 2023.08.31
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Abstract

This study was conducted to provide basic data on the antioxidant activity, inhibition of adipocyte differentiation and reactive oxygen species (ROS) production of a mixture of Brassica juncea extract (BJE) and fermented black rice fraction (BRF). We investigated the total phenol content, total flavonoid content, antioxidant effects (DPPH radical scavenging, ABTS radical scavenging, reducing power, FRAP and ORAC assay) and anti-obesity activity of the mixture in 3T3-L1 cells. Our results showed that the total phenol and flavonoid content increased with increasing BRF mixture ratio. The antioxidant activity increased as the BRF mixture ratio increased. In addition, BJE and BRF mixtures did not show any cytotoxicity during the 3T3-L1 differentiation period. During adipocyte differentiation, BJE and BRF mixtures significantly inhibited lipid accumulation and ROS production compared to the control group. These results warrant further experiments to develop an anti-obesity functional food using a mixture of BJE and BRF.

Keywords

Acknowledgement

본 논문은 과학기술정보통신부와 연구개발특구진흥재단의 "연구개발특구육성(R&D, 2022-IT-RD-0236-01)" 사업의 지원 받아 수행된 연구로 이에 감사드립니다.

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